本文關(guān)鍵詞： miRNA-a-p 骨肉瘤 增殖 周期 自噬　出處：《腫瘤防治研究》2017年03期 　論文類型：期刊論文

【摘要】：目的探討mi RNA-199a-5p對人骨肉瘤細胞株U2OS增殖及自噬的影響及其可能的作用機制。方法實時定量PCR法檢測32例骨肉瘤患者的病理組織標本及配對癌旁正常組織中mi RNA-199a-5p的表達水平。將mi RNA-199a-5p mimics轉(zhuǎn)染入U2OS細胞,使其過表達mi RNA-199a-5p,采用CCK-8法和流式細胞儀檢測細胞增殖及周期的變化;Western blot檢測周期相關(guān)蛋白(cyclin D1、P27、p Rb)及自噬相關(guān)蛋白(Beclin 1、LC3Ⅱ/Ⅰ)表達水平。結(jié)果 mi RNA-199a-5p在骨肉瘤組織及癌細胞U2OS中低表達,且表達水平與腫塊大小、遠距轉(zhuǎn)移、Enneking臨床分期及病理分級密切相關(guān)。過表達mi RNA-199a-5p后,U2OS細胞的增殖能力顯著降低(P0.05),同時cyclin D1、p Rb表達水平顯著下降,而P27、LC3Ⅱ/Ⅰ、Beclin 1的表達水平顯著增加(P0.05)。結(jié)論 mi RNA-199a-5p能夠抑制人骨肉瘤細胞株U2OS的增殖并激活細胞自噬;其作用機制可能與下調(diào)cyclin D1、上調(diào)P27、抑制p Rb蛋白的磷酸化、造成細胞周期G1/S阻滯有關(guān)。提示mi RNA-199a-5p可以作為骨肉瘤臨床治療的潛在作用位點。
[Abstract]:Objective to investigate the effect of mi RNA-199a-5p on proliferation and autophagy of human osteosarcoma cell line U2OS and its possible mechanism. Methods Real-time quantitative PCR assay was used to detect the expression of mi RNA-199a-5p in pathological tissues of 32 patients with osteosarcoma and matched adjacent normal tissues. Transfection of mi RNA-199a-5p mimics into U2OS cells, CCK-8 assay and flow cytometry were used to detect cell proliferation and cell cycle changes. Western blot was used to detect the expression levels of cyclin D1P27pRband autophagy associated protein (Beclin 1) LC3 鈪，

本文編號：1539877