本文關(guān)鍵詞： 辣椒堿 乳腺癌 遷移 侵襲 MDA-MB-細(xì)胞　出處：《生理學(xué)報(bào)》2017年02期 　論文類型：期刊論文

【摘要】：本研究旨在觀察辣椒堿對(duì)人乳腺癌MDA-MB-231細(xì)胞遷移和侵襲的影響,并探討其分子機(jī)制。乳腺癌MDA-MB-231細(xì)胞經(jīng)不同濃度辣椒堿作用24 h后進(jìn)行實(shí)驗(yàn),采用CCK-8法檢測(cè)細(xì)胞活性,采用細(xì)胞劃痕實(shí)驗(yàn)測(cè)量細(xì)胞遷移率,采用Transwell侵襲實(shí)驗(yàn)檢測(cè)細(xì)胞侵襲率,采用Western blot檢測(cè)乳腺癌MDA-MB-231細(xì)胞中c-Src、p-c-Src(Tyr416)、黏著斑激酶(focal adhesion kinase,FAK)、p-FAK(Tyr576/577)、樁蛋白(Paxillin)、p-Paxillin(Tyr118)、基質(zhì)金屬蛋白酶2(matrix metalloproteinase 2,MMP2)和MMP9蛋白表達(dá)水平,采用RT-PCR法檢測(cè)乳腺癌MDA-MB-231細(xì)胞中MMP2和MMP9的mRNA水平。結(jié)果顯示,10~50μmol/L辣椒堿對(duì)MDA-MB-231細(xì)胞存活率無顯著影響,100~500μmol/L辣椒堿顯著降低MDA-MB-231細(xì)胞存活率(P0.05);與正常對(duì)照組比較,25和50μmol/L辣椒堿組MDA-MB-231細(xì)胞體外遷移率和侵襲率均顯著降低(P0.05),c-Src、FAK和Paxillin蛋白的磷酸化水平均顯著下降(P0.05),MMP2和MMP9的mRNA和蛋白表達(dá)水平均下降(P0.05);并且辣椒堿對(duì)MDA-MB-231細(xì)胞的上述作用具有劑量依賴性。以上結(jié)果提示,低濃度辣椒堿可劑量依賴性地抑制人乳腺癌MDA-MB-231細(xì)胞遷移和侵襲,其機(jī)制可能涉及辣椒堿對(duì)c-Src/FAK/Paxillin信號(hào)通路以及MMP2和MMP9表達(dá)的抑制。
[Abstract]:The purpose of this study was to observe the effect of capsaicin on the migration and invasion of human breast cancer MDA-MB-231 cells, and to explore its molecular mechanism. After 24 h of different concentrations of capsaicin, the cell activity was detected by CCK-8 assay. Cell migration was measured by cell scratch assay, cell invasion rate was detected by Transwell invasion assay, expression levels of MMP9 protein and c-Srcnp-c-Srctr Tyr416 in breast cancer MDA-MB-231 cells, focal adhesion kinase- pFAK tyr57657 / 7, Paxillinp-Paxillin Tyr118, matrix metalloproteinase 2 metalloproteinase 2MMP2) and MMP9 protein expression were detected by Western blot. The mRNA levels of MMP2 and MMP9 in breast cancer MDA-MB-231 cells were detected by RT-PCR assay. The results showed that capsaicin 1050 渭 mol/L had no significant effect on the survival rate of MDA-MB-231 cells. Capsaicin 100 渭 mol/L significantly decreased the survival rate of MDA-MB-231 cells P0.05, and capsaicin 25 and 50 渭 mol/L capsaicin compared with the normal control group. The in vitro migration and invasion of MDA-MB-231 cells in vitro decreased the phosphorylation level of P0.05, c-Srcfak and Paxillin proteins, and the mRNA and protein expression levels of P0.05MMP2 and MMP9 decreased in a dose-dependent manner, and capsaicin had a dose-dependent effect on MDA-MB-231 cells. The above results suggest that, Low concentration capsaicin can inhibit the migration and invasion of human breast cancer MDA-MB-231 cells in a dose-dependent manner. The mechanism may be related to the inhibition of capsaicin on the c-Src / FAK / paxillin signaling pathway and the expression of MMP2 and MMP9.
【作者單位】： 漯河醫(yī)學(xué)高等�？茖W(xué)校分子醫(yī)學(xué)實(shí)驗(yàn)室;
【基金】：supported by the Science and Technology Development Project of the Department of Science and Technology of Henan Province,China(No.142102310466) the Natural Science Research Project of Luohe Medical College,Henan Province,China(No.2014-S-LMC09)
【分類號(hào)】：R737.9

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 朱劍梅;金科;;辣椒堿對(duì)結(jié)腸癌細(xì)胞自噬抑制作用的研究[J];腫瘤學(xué)雜志;2014年06期

相關(guān)重要報(bào)紙文章 前1條

1 記者 邰舉;韓揭示辣椒堿促發(fā)癌癥機(jī)理[N];科技日?qǐng)?bào);2010年

，

本文編號(hào)：1528214