本文關(guān)鍵詞： 肝癌 干擾素調(diào)節(jié)因子7 增殖 周期 侵襲　出處：《南昌大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：研究目的:分析IRF7基因在肝癌與癌旁組織中的表達差異,并結(jié)合患者臨床資料,探討其表達與肝癌發(fā)生發(fā)展的關(guān)系;通過降低IRF7的表達,觀察其對肝癌細胞生物學(xué)行為的影響,進一步了解IRF7基因影響肝癌進展的機制,從而為肝癌病變機制提供一定實驗和理論依據(jù)。第一部分:IRF7在肝癌組織中的表達及與臨床預(yù)后的關(guān)系實驗?zāi)康?分析IRF7基因在肝癌及相應(yīng)癌旁組織中的表達差異,并探討其表達與肝癌臨床預(yù)后的關(guān)系。實驗方法:1、應(yīng)用Western Blot及免疫組織化學(xué)方法檢測IRF7在肝癌及相應(yīng)癌旁組織中表達。2、結(jié)合患者臨床資料,分析IRF7基因在肝癌組織中的表達與肝癌患者性別、年齡、血清AFP、肝硬化、腫瘤大小、腫瘤分期、腫瘤轉(zhuǎn)移等臨床相關(guān)特征之間的關(guān)系。實驗結(jié)果:1、Western Blot檢測結(jié)果:在所檢測的40對肝癌組織及相應(yīng)的癌旁肝組織中,29例(72.5%)肝癌組織中IRF7蛋白表達水平比相應(yīng)的癌旁組織明顯升高,11例(27.5%)癌旁組織中IRF7蛋白表達高于癌組織。2、免疫組織化學(xué)檢測結(jié)果:在60例肝癌組織中IRF7高表達為65.0%(39/60),癌旁組織中高表達率為38.3%(23/60),IRF7在肝癌組織中高表達率明顯高于癌旁組織高表達率,并具有統(tǒng)計學(xué)意義(P0.05)。3、分析IRF7的表達與各臨床參數(shù)的關(guān)系,發(fā)現(xiàn)IRF7的表達與腫瘤的大小、轉(zhuǎn)移呈正相關(guān),與分化程度呈負(fù)相關(guān)。結(jié)論:IRF7基因在肝癌組織中高表達,且其表達與肝癌的侵襲和惡性程度相關(guān),在肝癌的發(fā)生發(fā)展中起到重要的作用。第二部分:IRF7表達對肝癌細胞生物學(xué)功能的影響實驗?zāi)康?通過細胞實驗進一步研究IRF7基因的表達對肝癌細胞增殖、周期、侵襲的影響。實驗方法:1、q RT-PCR和Western Blot方法檢測正常肝臟細胞株HL-7702,及不同的肝癌細胞系(Hep G2,Huh-7,Hep3B、SMMC7721,MHCCLM3)中目的基因IRF7的表達。2、RNA干擾的方法特異性抑制了肝癌細胞中IRF7基因的表達,分別應(yīng)用CCK8、流式細胞儀技術(shù)、Transwell法檢測降低IRF7基因表達后對肝癌細胞增殖、細胞周期、侵襲轉(zhuǎn)移能力的影響。實驗結(jié)果:1、q RT-PCR與Western Blot結(jié)果提示,IRF7 mRNA和蛋白表達在各肝癌細胞系中的表達均比正常肝細胞高;且在肝癌細胞株MHCCLM3中IRF7表達較其他肝癌細胞系更高。2、RNA干擾MHCCLM3肝癌細胞株中IRF7的表達,qRT-PCR和Western Blot檢測IRF7在mRNA和蛋白表達水平的干擾效果,并篩選出最佳的RNA干擾系列。3、CCK8檢測、流式細胞儀技術(shù)、Transwell法檢測發(fā)現(xiàn),降低IRF7基因的表達,肝癌細胞增殖,周期及侵襲明顯受到抑制。結(jié)論:IRF7基因在肝癌細胞株高表達。IRF7的表達能夠促進肝癌細胞增殖、周期及侵襲�？偨Y(jié):本課題通過檢測肝癌組織中IRF7的表達,并分析其與肝癌患者的臨床資料,發(fā)現(xiàn)IRF7的表達與肝癌的發(fā)生發(fā)展密切相關(guān)。同時,通過細胞生物學(xué)功能實驗,證實了IRF7的表達能夠促進肝癌細胞增殖、周期、轉(zhuǎn)移;進一步的揭示了IRF7基因的表達在肝癌的進展中發(fā)揮了重要的作用。
[Abstract]:Objective: to analyze the difference of IRF7 gene expression between HCC and paracancerous tissues, and to explore the relationship between the expression of IRF7 gene and the occurrence and development of HCC, and to observe the effect of IRF7 on the biological behavior of HCC cells. To further understand the mechanism of IRF7 gene affecting the progression of liver cancer, The first part is the expression of IRF7 gene in HCC tissues and its relationship with clinical prognosis. Objective: to analyze the difference of expression of IRF7 gene in HCC and its adjacent tissues. To investigate the relationship between the expression of IRF7 and the clinical prognosis of HCC, we used Western Blot and immunohistochemical method to detect the expression of IRF7 in HCC and its adjacent tissues, and combined with the clinical data of the patients. To analyze the expression of IRF7 gene in hepatocellular carcinoma (HCC) and its relationship with sex, age, serum AFP, liver cirrhosis, tumor size and tumor staging. The relationship between tumor metastasis and other clinicopathological characteristics. Results: the expression of IRF7 protein in 40 pairs of HCC tissues and corresponding adjacent liver tissues was higher than that in corresponding paracancerous tissues. The expression of IRF7 protein in adjacent tissues was significantly higher than that in cancer tissues. The results of immunohistochemistry: in 60 cases of liver cancer, the high expression rate of IRF7 was 65.039 / 60%, and the high expression rate of 38.3% / 2360% IRF7 was higher in HCC tissues. The rate of high expression was significantly higher than that of paracancerous tissues. It was found that the expression of IRF7 was positively correlated with tumor size and metastasis, and negatively correlated with the degree of differentiation. Conclusion the expression of IRF7 gene is highly expressed in hepatocellular carcinoma. And its expression is related to the invasion and malignancy of liver cancer. The second part is the effect of the expression of IRF7 7 on the biological function of hepatoma cells. Objective: to further study the effect of IRF7 gene expression on the proliferation and cycle of hepatoma cells through cell experiments. The expression of IRF7 gene in HL-7702 cells and different hepatoma cell lines Hep G2H Huh-7h-7, Hep3BSS-SMMC7721 MHCCLM3 was detected by using the methods of 1: 1Q RT-PCR and Western Blot, which specifically inhibited the expression of IRF7 gene in hepatoma cells. CCK8 and flow cytometry were used to detect the proliferation and cell cycle of hepatoma cells after IRF7 gene expression was reduced. The results showed that the expression of IRF7 mRNA and protein in HCC cells was higher than that in normal hepatocytes. Moreover, the expression of IRF7 in HCC cell line MHCCLM3 was higher than that in other HCC cell lines. The expression of IRF7 in MHCCLM3 cell line was interfered with qRT-PCR and Western Blot were used to detect the interference effect of IRF7 on mRNA and protein expression, and the best RNA interference series. Flow cytometry analysis showed that the expression of IRF7 gene decreased, the proliferation, cycle and invasion of HCC cells were inhibited obviously. Conclusion the high expression of% IRF7 gene in hepatoma cells can promote the proliferation of HCC cells. Period and invasion. Summary: by detecting the expression of IRF7 in HCC and analyzing the clinical data of HCC patients, we found that the expression of IRF7 is closely related to the occurrence and development of HCC. At the same time, through the experiment of cell biological function, we found that the expression of IRF7 is closely related to the occurrence and development of HCC. It is confirmed that the expression of IRF7 can promote the proliferation, cycle and metastasis of HCC cells, and further reveal that the expression of IRF7 gene plays an important role in the progression of HCC.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R735.7

【參考文獻】

相關(guān)期刊論文 前3條

1 齊月;金清龍;溫曉玉;�？∑�;;肝細胞中固有干擾素調(diào)節(jié)因子7的表達及其抗病毒作用[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2012年02期

2 繆倩;唐元家;黃新芳;錢曉霞;黃秀琴;沈南;;IRF7表達與系統(tǒng)性紅斑狼瘡的相關(guān)性[J];華東師范大學(xué)學(xué)報(自然科學(xué)版);2010年04期

3 張婷;王香玲;紀(jì)玉強;;外周血單個核細胞IRF-7 mRNA水平與乙肝病毒慢性感染關(guān)系的研究[J];現(xiàn)代檢驗醫(yī)學(xué)雜志;2008年01期

，

本文編號：1522874