本文關(guān)鍵詞： 血根堿 舌癌 增殖 凋亡 遷移　出處：《中藥藥理與臨床》2016年05期 　論文類型：期刊論文

【摘要】：目的:研究博落回提取物中血根堿(Sanguinarine,SAN)對舌癌體外增殖、凋亡和遷移作用的影響及其機(jī)制。方法:采用不同濃度的血根堿處理Tscca和Tca8113細(xì)胞24、48、72 h,MTT法檢測血根堿對兩種舌癌細(xì)胞增殖抑制的影響;用藥48 h后,流式細(xì)胞儀Annexin V-FITC/PI染色法檢測不同濃度血根堿對細(xì)胞凋亡的影響;Western blotting法檢測不同濃度血根堿對細(xì)胞中NF-κB表達(dá)的影響;細(xì)胞劃痕實(shí)驗(yàn)檢測不同濃度血根堿對舌癌細(xì)胞遷移能力的影響。結(jié)果:經(jīng)血根堿處理后的舌癌細(xì)胞系Tscca和Tca8113有明顯的抑制增殖作用,并呈現(xiàn)一定的濃度和時(shí)間依賴關(guān)系。血根堿作用于舌鱗癌Tca8113細(xì)胞24 h、48 h、72h的半數(shù)抑制濃度(IC50)分別為2.53μmol/L、2.38μmol/L、1.96μmol/L。舌鱗癌Tscca細(xì)胞24 h、48 h、72h的半數(shù)抑制濃度(IC_(50))分別為1.48μmol/L、1.17μmol/L、0.89μmol/L。血根堿可以抑制NF-κB的表達(dá),且有一定的劑量相關(guān)性。一定濃度的血根堿還能抑制Tca8113細(xì)胞株的遷移。結(jié)論:血根堿能顯著抑制舌癌細(xì)胞Tscca和Tca8113的增殖并且誘導(dǎo)其凋亡,其機(jī)制可能與對NF-κB表達(dá)的影響有關(guān),同時(shí)血根堿可影響Tca8113細(xì)胞株的遷移。
[Abstract]:Objective: to study the effect of Sanguinarine (SAN) on the proliferation of tongue cancer in vitro. Methods: Tscca and Tca8113 cells were treated with different concentrations of hematopoietic alkaloids for 24 872 h, and the effects of root alkaloids on the proliferation of two kinds of tongue cancer cells were detected by MTT assay. The effect of different concentrations of root alkaloids on apoptosis was detected by flow cytometry (Annexin V-FITC / Pi staining). Western blotting was used to detect the effect of different concentrations of root alkaloids on the expression of NF- 魏 B in cells. The effect of different concentrations of root alkaloids on the migration ability of tongue cancer cells was detected by cell scratch assay. Results: the proliferation of tongue cancer cell lines Tscca and Tca8113 treated by root alkaloids was significantly inhibited. IC50 was 2.53 渭 mol / L / L 2.38 渭 mol / L 1.96 渭 mol / L for 24 h / 48 h / 72 h, respectively. The IC50 of tongue squamous cell carcinoma Tscca cell line was 1.48 渭 mol / L 1.17 渭 mol / L 0.89 渭 mol 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) ~ 0.89 渭 mol 路L ~ (-1) 路L ~ (-1) ~ 0.89 渭 mol 路L ~ (-1) ~ 0.89 渭 mol 路L ~ (-1) 路L ~ (-1) ~ (-1) 渭 mol 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) ~ 0.89 渭 mol 路L ~ (-1)? And there was a dose-dependent relationship between them. The migration of Tca8113 cell line was inhibited by a certain concentration of root alkaloid. Conclusion: root alkaloid can significantly inhibit the proliferation and induce apoptosis of Tscca and Tca8113 in tongue cancer cells, and its mechanism may be related to the effect on the expression of NF- 魏 B. At the same time, root alkaloids could affect the migration of Tca8113 cell lines.
【作者單位】： 錦州醫(yī)科大學(xué);錦州醫(yī)科大學(xué)附屬一院變態(tài)及過敏反應(yīng)實(shí)驗(yàn)室;錦州醫(yī)科大學(xué)附屬二院正畸科;
【基金】：國家自然科學(xué)基金(81471592) 遼寧省教育廳科學(xué)研究項(xiàng)目(05L141)
【分類號】：R285

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