發(fā)布時(shí)間：2018-02-15 15:07

  本文關(guān)鍵詞： 神經(jīng)膠質(zhì)瘤 microRNA- B細(xì)胞淋巴瘤/白血病-基因 增殖 凋亡　出處：《中國現(xiàn)代醫(yī)學(xué)雜志》2017年22期 　論文類型：期刊論文

【摘要】：目的探討micro RNA-25(mi R-25)表達(dá)下調(diào)對神經(jīng)膠質(zhì)瘤U87細(xì)胞增殖與凋亡的影響,以及mi R-25與B細(xì)胞淋巴瘤/白血病-2基因(Bcl-2)在膠質(zhì)瘤細(xì)胞增殖與凋亡過程中的調(diào)節(jié)作用。方法通過慢病毒介導(dǎo)反義mi R-25寡聚核苷酸轉(zhuǎn)染U87細(xì)胞;分為3組,實(shí)驗(yàn)組(轉(zhuǎn)染anti-mi R-25)、對照組(轉(zhuǎn)染negative control)和空白組(空白PBS);實(shí)時(shí)熒光定量聚合酶鏈反應(yīng)(q RT-PCR)檢測mi R-25和Bcl-2的m RNA表達(dá)水平;CCK-8法檢測細(xì)胞增殖情況;流式細(xì)胞術(shù)檢測細(xì)胞周期和細(xì)胞凋亡。結(jié)果實(shí)驗(yàn)組mi R-25和Bcl-2的m RNA表達(dá)量與空白組和對照組比較,差異具有統(tǒng)計(jì)學(xué)意義(P0.05),實(shí)驗(yàn)組降低;實(shí)驗(yàn)組相對于空白組和對照組,細(xì)胞增殖活性降低,細(xì)胞周期延緩,細(xì)胞凋亡率升高。結(jié)論在膠質(zhì)瘤U87細(xì)胞,mi R-25表達(dá)下調(diào)通過作用于Bcl-2靶基因,延緩細(xì)胞周期,抑制細(xì)胞增殖,促進(jìn)細(xì)胞凋亡。
[Abstract]:Objective to investigate the effect of down-regulation of micro RNA-25(mi R-25 on proliferation and apoptosis of glioma U87 cells. And the role of mi R-25 and B cell lymphoma / leukemia 2 gene Bcl-2 in the proliferation and apoptosis of glioma cells. Methods U87 cells were transfected with antisense miR-25 oligonucleotides mediated by lentivirus and divided into three groups. In the experimental group (transfected with anti-mi R-25), the control group (transfected with negative control) and the blank group (control group), the expression level of m RNA of miR-25 and Bcl-2 was detected by real-time quantitative polymerase chain reaction (RT-PCR) and the cell proliferation was detected by CCK-8 method. Results the expression of m RNA of miR-25 and Bcl-2 in the experimental group was significantly lower than that in the blank group and the control group (P 0.05), while the expression of m RNA in the experimental group was lower than that in the blank group and the control group. Conclusion in U87 glioma cells, the down-regulated expression of miR-25 inhibits cell cycle, inhibits cell proliferation and promotes apoptosis by acting on Bcl-2 target gene.
【作者單位】： 華北理工大學(xué)附屬醫(yī)院神經(jīng)外科;河北醫(yī)科大學(xué)附屬邢臺市人民醫(yī)院神經(jīng)外科;
【分類號】：R739.41

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