本文關(guān)鍵詞： 胃癌 腫瘤干細胞 CD 球形體　出處：《中華腫瘤防治雜志》2017年03期 　論文類型：期刊論文

【摘要】：目的腫瘤干細胞(cancer stem cells,CSCs)在惡性腫瘤的發(fā)生發(fā)展中起著重要作用,且是腫瘤耐藥及復發(fā)的根源。本研究探討CSCs表面標記蛋白CD44在分離及鑒定胃癌干細胞中的重要作用。方法收集2015-11-06-2015-12-01華中科技大學同濟醫(yī)學院附屬同濟醫(yī)院3例胃癌患者手術(shù)標本。首先采用膠原酶消化法獲取胃癌細胞,部分細胞分別貼壁及懸浮培養(yǎng),并采用免疫熒光及流式分析的方法檢測胃癌球形體中CD44的表達情況。部分細胞采用流式細胞儀分選得到CD44~+及CD44~-胃癌細胞,并通過無血清懸浮培養(yǎng)法比較兩群細胞的球形體形成能力。結(jié)果免疫熒光顯示,CD44蛋白在球形體中高表達;流式分析發(fā)現(xiàn),貼壁的原代胃癌細胞中CD44~+細胞的比例為(30.73±7.6)%,而球形體中CD44~+細胞的比例高達(56.4±5.58)%,差異有統(tǒng)計學意義,P=0.009。球形體形成實驗結(jié)果表明,與CD44~-細胞相比,CD44~+胃癌細胞具有更強的球形體形成能力,1 000個CD44~+及CD44~-胃癌細胞分別形成(14.33±2.52)和(1.67±0.58)個球形體,差異有統(tǒng)計學意義,P=0.001。通過比較發(fā)現(xiàn),球形體培養(yǎng)對胃癌干細胞的富集效率為0.3%,而CD44對胃癌干細胞的富集效率為1.4%。結(jié)論 CD44是胃癌干細胞的表面標記蛋白,其可能成為胃癌靶向治療的可靠靶標。
[Abstract]:Objective Cancer stem cell stem cells play an important role in the occurrence and development of malignant tumor. In this study, we investigated the important role of CSCs surface marker protein CD44 in the isolation and identification of gastric cancer stem cells. Methods 2015-11-06-2015-. 12-01 gastric cancer cells were obtained by collagenase digestion from 3 patients with gastric cancer in Tongji Hospital affiliated to Tongji Medical College of Huazhong University of Science and Technology. Some of the cells were adhered to the wall and cultured in suspension. The expression of CD44 in the spherical body of gastric cancer was detected by immunofluorescence and flow cytometry. CD44 ~ and CD44 ~-gastric cancer cells were isolated by flow cytometry. The ability of spheroid formation of the two groups of cells was compared by serum-free suspension culture. Results Immunofluorescence showed that CD44 protein was highly expressed in the spheroidium. The percentage of CD44 ~ cells in primary gastric cancer cells was 30.73 鹵7.6%. The percentage of CD44~ cells in the globular body was 56.4 鹵5.58. The difference was statistically significant. Compared with CD44- cells, CD44- cells have stronger spheroidogenesis ability. 1 000 CD44 ~ and CD44 ~-gastric cancer cells formed 14. 33 鹵2. 52) and 1. 67 鹵0. 58) spheroids, respectively. The difference was statistically significant. P0. 001. by comparison, it was found that the enrichment rate of gastric cancer stem cells in spheroid culture was 0.3%. Conclusion CD44 is the surface marker protein of gastric cancer stem cells, and it may be a reliable target for gastric cancer targeted therapy.
【作者單位】： 華中科技大學同濟醫(yī)學院附屬同濟醫(yī)院腫瘤中心;
【基金】：國家自然科學基金(81201820)
【分類號】：R735.2
【正文快照】： 中華腫瘤防治雜志,2017,24(3):173-177Chin J Cancer Prev Treat,2017,24(3):173-177自從20世紀末腫瘤干細胞(cancer stem cells,CSCs)理論被提出后,其在很大程度上改變了惡性腫瘤的臨床診斷思維和治療方法。該理論認為CSCs是腫瘤組織中存在的一小群具有干細胞的自我更新和分

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