發(fā)布時(shí)間：2018-01-26 05:27

  本文關(guān)鍵詞： MUC1 β-catenin 食管鱗癌　出處：《川北醫(yī)學(xué)院》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的:通過(guò)檢測(cè)β-連環(huán)蛋白(β-catenin,β-cat)、粘蛋白1(mucin1,MUC1)在正常食管粘膜、食管粘膜上皮內(nèi)瘤變及不同臨床分期食管鱗癌中的表達(dá),探討β-catenin、MUC1與食管癌發(fā)生發(fā)展的相關(guān)性,從而為食管癌早期診斷、推測(cè)預(yù)后提供參考;并為探索食管癌治療的新靶點(diǎn)提供初步的理論依據(jù)。方法:采用免疫組織化學(xué)染色檢測(cè)正常食管粘膜、食管粘膜上皮內(nèi)瘤變及不同臨床分期食管鱗癌中β-catenin、MUC1的表達(dá)水平。用SPSS13.0統(tǒng)計(jì)學(xué)軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果:1.β-catenin在正常食管粘膜、食管粘膜上皮內(nèi)瘤變、食管癌中陽(yáng)性表達(dá)率逐漸升高,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);低級(jí)別上皮內(nèi)瘤變組陽(yáng)性表達(dá)率顯著高于正常食管粘膜組(P0.05);低級(jí)別上皮內(nèi)瘤變組與高級(jí)別上皮內(nèi)瘤變組間、食管粘膜瘤樣變組與食管癌組間差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。在食管癌組中,腫瘤≥3cm組陽(yáng)性表達(dá)率顯著高于腫瘤3cm組,累及肌層和外膜層組陽(yáng)性表達(dá)率顯著高于局限粘膜及粘膜下層組,有淋巴結(jié)轉(zhuǎn)移組陽(yáng)性表達(dá)率顯著高于無(wú)淋巴結(jié)轉(zhuǎn)移組,腫瘤分期III+IV組陽(yáng)性表達(dá)率顯著高于腫瘤I+II組,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。2.MUC1在正常食管粘膜、食管粘膜上皮內(nèi)瘤變、食管癌中陽(yáng)性表達(dá)率逐漸升高,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);高級(jí)別上皮內(nèi)瘤變組陽(yáng)性表達(dá)率顯著高于正常食管粘膜組(P0.05);低級(jí)別上皮內(nèi)瘤變組與正常食管粘膜組間、低級(jí)別上皮內(nèi)瘤變組與高級(jí)別上皮內(nèi)瘤變組間、食管粘膜瘤樣變組與食管癌組間差異無(wú)統(tǒng)計(jì)學(xué)意義(p0.05)。在食管癌組中,腫瘤≥3cm組陽(yáng)性表達(dá)率顯著高于腫瘤3cm組,累及肌層和外膜層組陽(yáng)性表達(dá)率顯著高于局限粘膜及粘膜下層組,有淋巴結(jié)轉(zhuǎn)移組陽(yáng)性表達(dá)率顯著高于無(wú)淋巴結(jié)轉(zhuǎn)移組,腫瘤分期iii+iv組陽(yáng)性表達(dá)率顯著高于腫瘤i+ii組,差異均有統(tǒng)計(jì)學(xué)意義(p0.05)。3.在正常食管粘膜組、食管粘膜上皮內(nèi)瘤變組、食管癌組中,β-catenin、muc1共同陽(yáng)性表達(dá)率逐漸升高,差異具有統(tǒng)計(jì)學(xué)意義(p0.05),低級(jí)別上皮內(nèi)瘤變組共同陽(yáng)性表達(dá)率顯著高于正常食管粘膜組(p0.05),低級(jí)別上皮內(nèi)瘤變組與高級(jí)別上皮內(nèi)瘤變組間、食管粘膜瘤樣變組與食管癌組間差異無(wú)統(tǒng)計(jì)學(xué)意義(p0.05)。β-catenin、muc1在正常食管粘膜組、食管粘膜上皮內(nèi)瘤變、食管癌組中呈正相關(guān)(p0.01)。在食管癌組,β-catenin、muc1共同陽(yáng)性表達(dá)率在累及肌層和外膜層組陽(yáng)性表達(dá)率顯著高于局限粘膜及粘膜下層組,有淋巴結(jié)轉(zhuǎn)移組陽(yáng)性表達(dá)率顯著高于無(wú)淋巴結(jié)轉(zhuǎn)移組,腫瘤分期iii+iv組陽(yáng)性表達(dá)率顯著高于腫瘤i+ii組,差異均有統(tǒng)計(jì)學(xué)意義(p0.05)。結(jié)論:1.β-catenin、muc1的異常表達(dá)與食管粘膜上皮內(nèi)瘤變及食管癌的發(fā)生密切相關(guān),可能是食管癌發(fā)生的重要分子機(jī)制之一。2.在食管粘膜上皮內(nèi)瘤變中,β-catenin、muc1異常表達(dá)顯著高于正常食管粘膜,二者作為分子標(biāo)記,對(duì)食管粘膜癌前病變的早期篩查可能有潛在的臨床應(yīng)用價(jià)值。3.β-catenin、muc1的陽(yáng)性表達(dá)與腫瘤大小、腫瘤分期、浸潤(rùn)程度、淋巴結(jié)轉(zhuǎn)移關(guān)系密切。提示β-catenin、muc1與腫瘤的轉(zhuǎn)移、侵襲有關(guān),對(duì)判斷食管癌的預(yù)后有一定的價(jià)值。4.β-catenin、MUC1的共同陽(yáng)性表達(dá)率在食管粘膜瘤樣病變及食管癌組織中明顯高于正常食管粘膜,二者在食管癌的發(fā)生發(fā)展中可能起協(xié)同作用。針對(duì)β-catenin、MUC1的基因或免疫治療或許是食管癌治療的新靶點(diǎn),值得進(jìn)一步研究。
[Abstract]:Objective: through the detection of beta catenin (-catenin beta, beta -cat), mucin 1 (Mucin1, MUC1) in normal esophageal mucosa, the expression of esophageal intraepithelial neoplasia and esophageal squamous cell carcinoma in different clinical stages, to explore the development of beta -catenin, relationship between MUC1 and esophageal cancer, and early diagnosis of esophageal cancer the prognosis, to provide reference; and provide the theoretical basis for the exploration of new targets for treatment of esophageal carcinoma. Methods: immunohistochemical staining of normal esophageal mucosa, esophageal intraepithelial neoplasia and esophageal squamous cell carcinoma in different clinical stages of beta -catenin, the expression level of MUC1. Statistical analysis was performed by SPSS13.0 statistical software. 1.: beta -catenin in normal esophageal mucosa, esophageal intraepithelial neoplasia, the positive expression rate of esophageal cancer is gradually increased, the difference was statistically significant (P0.05); low grade intraepithelial neoplasia group significant positive expression rate Higher than normal esophageal mucosa group (P0.05); low-grade intraepithelial neoplasia and high-grade intraepithelial neoplasia groups, esophageal mucosal neoplasia and esophageal cancer. The difference between groups was not statistically significant (P0.05). In esophageal carcinoma group, tumor group than 3cm positive expression rate was significantly higher than that of tumor 3cm group. Involving the muscular layer and adventitia group positive expression rate was significantly higher than that of the limitations of the mucosa and submucosa group, the group with lymph node metastasis positive expression rate was significantly higher than that in the group without lymph node metastasis, tumor stage III+IV group positive expression rate was significantly higher than that of tumor in I+II group, there were statistically significant differences (P0.05).2.MUC1 in normal esophageal mucosa and esophageal mucosa intraepithelial neoplasia, the positive expression rate of esophageal cancer is gradually increased, the difference was statistically significant (P0.05); high grade intraepithelial neoplasia group positive expression rate was significantly higher than that in normal esophageal mucosa group (P0.05); low grade intraepithelial neoplasia group and normal. Tube mucosa group, low-grade intraepithelial neoplasia and high-grade intraepithelial neoplasia groups, esophageal mucosal neoplasia and esophageal cancer. The difference between groups was not statistically significant (P0.05). In esophageal carcinoma group, tumor group than 3cm positive expression rate was significantly higher than that of tumor group 3cm, involving the muscular layer and the outer layer group positive expression rate was significantly higher than that of the limitations of the mucosa and submucosa group, the group with lymph node metastasis positive expression rate was significantly higher than that in the group without lymph node metastasis, tumor stage iii+iv group positive expression rate was significantly higher than that of tumor in i+ii group, there were statistically significant differences (P0.05).3. in normal esophageal mucosa, mucosa of esophageal intraepithelial neoplasia group -catenin beta esophageal cancer group, MUC1, positive expression rate gradually increased, the difference was statistically significant (P0.05), low grade intraepithelial neoplasia group positive expression rate was significantly higher than that in normal esophageal mucosa group (P0.05), low grade intraepithelial neoplasia The group with high grade intraepithelial neoplasia group, esophageal mucosal neoplasia and esophageal cancer. The difference between groups was not statistically significant (P0.05). Beta -catenin, MUC1 in normal esophageal mucosa, esophageal intraepithelial neoplasia and esophageal cancer was positively related to group (P0.01). In the esophageal cancer group, P -catenin MUC1, positive expression rate in the involved muscle layer and adventitia group positive expression rate was significantly higher than that of the limitations of the mucosa and submucosa group, the group with lymph node metastasis positive expression rate was significantly higher than that in the group without lymph node metastasis, tumor stage iii+iv group positive expression rate was significantly higher than that of tumor i+ II group, the differences were statistically significant (P0.05). Conclusion: the abnormal expression of -catenin beta 1., MUC1 and esophageal epithelial neoplasia and esophageal cancer is closely related, may be an important molecular mechanism of esophageal cancer.2. in esophageal mucosa intraepithelial neoplasia, beta -catenin, abnormal expression of MUC1 was significantly higher In normal esophageal mucosa, two as molecular markers, may have clinical application value of.3. beta -catenin potential for early diagnosis of esophageal mucosa precancerous lesions, the positive expression of MUC1 with tumor size, tumor stage, tumor invasion, lymph node metastasis. It is suggested that beta -catenin, metastasis, MUC1 and tumor invasion. Have a certain value of.4. beta -catenin in prognosis of esophageal cancer, a common positive rate of expression of MUC1 in tissue of esophageal mucosa neoplasia and esophageal cancer was significantly higher than that of normal esophageal mucosa, two in esophageal carcinoma development may play a synergistic effect. The beta -catenin gene or MUC1 immune therapy may be a new target for the treatment of esophageal cancer, it is worthy of further study.

【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R735.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 Wanqing Chen;Rongshou Zheng;Hongmei Zeng;Siwei Zhang;Jie He;;Annual report on status of cancer in China, 2011[J];Chinese Journal of Cancer Research;2015年01期

2 施文榮;劉艷;;甲基芐基亞硝胺誘導(dǎo)大鼠食管癌癌前病變食管組織中β-catenin表達(dá)及其Ser675位點(diǎn)磷酸化水平的變化[J];癌變.畸變.突變;2014年05期

3 鄧華云;楊杰;趙克溫;黃雷;廖曉東;;MUC1在食管癌成瘤中的作用[J];外科理論與實(shí)踐;2014年05期

4 李秀娟;李明霞;劉軍超;金春亭;范婕;李玉珍;;食管鱗癌組織β-catenin表達(dá)與淋巴管生成的關(guān)系[J];中國(guó)老年學(xué)雜志;2014年18期

5 余娜;梅武軒;;Wnt信號(hào)通路與消化道腫瘤研究進(jìn)展[J];中國(guó)醫(yī)療前沿;2013年17期

6 盛守寅;劉利松;殷瑞忠;閔長(zhǎng)國(guó);鄧健;;MUC1及MMP-7在食管癌組織中的表達(dá)及其臨床意義研究[J];中國(guó)醫(yī)療前沿;2013年10期

7 張繼紅;任立群;王彩芹;姜晶;宓淑芳;王紅;歷春;李西寧;溫春陽(yáng);;MUC1、MUC2在宮頸癌中的表達(dá)及其臨床意義[J];中國(guó)實(shí)驗(yàn)診斷學(xué);2012年07期

8 甄強(qiáng);高麗娜;劉家寶;;MUC-1與β-Catenin在賁門(mén)癌組織中的表達(dá)及意義[J];中國(guó)醫(yī)藥指南;2011年17期

9 陳靖;張京霞;趙曉軍;劉淑霞;張欣;;β-catenin、GSK3β在食管癌中的表達(dá)及其與食管癌浸潤(rùn)和轉(zhuǎn)移的關(guān)系[J];中國(guó)醫(yī)藥導(dǎo)刊;2011年01期

10 張偉;張明金;江道振;鄭向民;單成祥;劉晟;仇明;;大腸癌黏蛋白1和β-連環(huán)素聯(lián)合表達(dá)的臨床意義[J];中華實(shí)驗(yàn)外科雜志;2011年01期

相關(guān)碩士學(xué)位論文 前2條

1 孫智;黏蛋白1在食管鱗癌中的表達(dá)及其與臨床病理及預(yù)后的關(guān)系[D];山東大學(xué);2015年

2 張戈妹;E-cadherin、MUC1與食管癌預(yù)后關(guān)系的研究[D];河北醫(yī)科大學(xué);2006年

，

本文編號(hào)：1464802