本文關(guān)鍵詞：緊密連接相關(guān)蛋白在肺癌腦轉(zhuǎn)移瘤中的表達(dá)及臨床意義　出處：《南昌大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 肺癌腦轉(zhuǎn)移瘤 血腦屏障 免疫組織化學(xué) Claudin-5 Occludin Zo-1

【摘要】：目的:探討血-腦屏障Claudin-5、Occludin及Zo-1蛋白在肺癌腦轉(zhuǎn)移瘤組織和正常腦組織中的表達(dá)差異,并分析其臨床意義。方法:應(yīng)用Envision免疫組化法,檢測Claudin-5、Occludin及Zo-1在63例肺癌腦轉(zhuǎn)移瘤組織和28例正常腦組織中的表達(dá)情況,研究三種緊密連接蛋白在轉(zhuǎn)移瘤組織和正常組織中的不同表達(dá),并應(yīng)用SPSS軟件,分析轉(zhuǎn)移瘤中三種緊密連接蛋白表達(dá)水平與患者各種臨床病理因素(性別、年齡、轉(zhuǎn)移灶大小及個數(shù)、病理分型)、生存時間的關(guān)系。結(jié)果:1.Claudin-5蛋白主要表達(dá)于細(xì)胞膜中,其在正常腦組織、腦轉(zhuǎn)移瘤組織中的高表達(dá)率各為50%和25.4%,差異有統(tǒng)計學(xué)意義(χ2=5.310,P=0.021)。Claudin-5在肺癌腦轉(zhuǎn)移瘤病灶中的表達(dá)與患者的年齡及轉(zhuǎn)移灶個數(shù)有關(guān),差異有統(tǒng)計學(xué)意義,而與患者性別、腫塊直徑、原發(fā)灶病理學(xué)分型無統(tǒng)計學(xué)相關(guān)性。2.Occludin蛋白主要表達(dá)在細(xì)胞膜中,在正常腦組織、轉(zhuǎn)移瘤組織中的高表達(dá)率各為57.1%、34.9%,差異有統(tǒng)計學(xué)意義(χ2=3.936,P=0.047)。腦轉(zhuǎn)移瘤組織中Occludin的表達(dá)與患者的年齡及轉(zhuǎn)移灶個數(shù)有關(guān),與患者性別、腫塊直徑、原發(fā)灶病理學(xué)分型無關(guān),差別無統(tǒng)計學(xué)意義(P0.05)。3.胞質(zhì)附屬蛋白Zo-1在正常腦組織、轉(zhuǎn)移瘤組織中的高表達(dá)率各為75%、33.3%,兩者高表達(dá)率的差異有統(tǒng)計學(xué)意義(χ2=13.542,P0.001)。肺癌腦轉(zhuǎn)移瘤組織中Zo-1的表達(dá)與患者的年齡及轉(zhuǎn)移灶個數(shù)有關(guān)(P0.05),差別有統(tǒng)計學(xué)意義,而與患者性別、腫塊直徑、原發(fā)灶病理學(xué)分型無明顯相關(guān)性。4.Claudin-5、Occludin及Zo-1蛋白在高表達(dá)組的mOS較低表達(dá)組的mOS長,差別有統(tǒng)計學(xué)意義(P0.05)。5.采用Spearman相關(guān)分析提示,Claudin-5、Occludin及Zo-1在肺癌腦轉(zhuǎn)移瘤組織中的表達(dá),兩兩之間呈正相關(guān)(P0.05)。結(jié)論:與正常腦組織相比,血-腦屏障上的Claudin-5、Occludin及Zo-1蛋白在腦轉(zhuǎn)移瘤組織中呈低表達(dá),表達(dá)水平的高低與患者的生存時間有關(guān),提示緊密連接相關(guān)蛋白Claudin-5、Occludin、Zo-1表達(dá)下調(diào)在肺癌腦轉(zhuǎn)移瘤的發(fā)生發(fā)展中可能起著一定的作用。
[Abstract]:Objective: to investigate the expression of Claudin-5 Occludin and Zo-1 protein in brain metastases of lung cancer and normal brain tissues. Methods: Envision immunohistochemical method was used to detect Claudin-5. The expression of Occludin and Zo-1 in 63 cases of lung cancer brain metastases and 28 cases of normal brain tissues were studied. SPSS software was used to analyze the expression level of three kinds of tight junction protein in metastatic tumor and the clinicopathological factors (sex, age, size and number of metastatic foci, pathological classification). Results 1. Claudin-5 protein was mainly expressed in cell membrane. The high expression rates of Claudin-5 protein in normal brain tissues and brain metastases were 50% and 25.4% respectively. The expression of Claudin-5 in brain metastases of lung cancer was related to the age of patients and the number of metastatic foci. The difference was statistically significant, but there was no statistical correlation with sex, tumor diameter and pathological classification of primary lesion. 2.Occludin protein was mainly expressed in the cell membrane and normal brain tissue. The high expression rates in metastatic tumor tissues were 57.1 and 34.9respectively, the difference being statistically significant (蠂 2 / 3.936). The expression of Occludin in brain metastases was related to the age of patients and the number of metastatic foci, but not to sex, tumor diameter and pathological type of primary tumor. The high expression rate of cytoplasmic accessory protein Zo-1 in normal brain tissue and metastatic tumor tissue was 75% and 33 3% respectively. The difference of high expression rate between the two groups was statistically significant (蠂 2 = 13.542). The expression of Zo-1 in brain metastases of lung cancer was related to the age of patients and the number of metastatic foci. The difference was statistically significant, but it was related to the sex of patients and the diameter of tumor. There was no significant correlation between pathological classification of primary lesions. 4. Claudin-5 Occludin and Zo-1 protein were longer in mOS with high expression than those with low expression. The difference was statistically significant (P 0.05). Spearman correlation analysis suggested that Claudin-5. The expression of Occludin and Zo-1 in brain metastases of lung cancer was positively correlated with that of normal brain tissue (P 0.05). The expression of Claudin-5 cludin and Zo-1 protein in the blood-brain barrier was low in the brain metastases, and the expression level was related to the survival time of the patients. It is suggested that the down-regulation of the tight junction associated protein Claudin-5 Occludingnan Zo-1 may play a role in the development of brain metastases of lung cancer.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R734.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉成文,李平;肺癌腦轉(zhuǎn)移瘤的立體定向放射外科治療[J];中國肺癌雜志;2000年05期

2 王樹森;表現(xiàn)為卒中樣的肺癌腦轉(zhuǎn)移瘤11例臨床分析[J];北京醫(yī)學(xué);2001年02期

3 張宏偉;李光;王志宇;;立體定向放射治療肺癌腦轉(zhuǎn)移瘤200例療效分析[J];現(xiàn)代預(yù)防醫(yī)學(xué);2006年06期

4 左頻;羅林;倪煒;范耀東;司馬秀田;白剛;;肺癌腦轉(zhuǎn)移瘤51例綜合治療的探討[J];云南醫(yī)藥;2008年01期

5 劉圖強(qiáng);莫如康;鐘志聯(lián);;肺癌腦轉(zhuǎn)移瘤放射治療臨床分析[J];中國實用醫(yī)藥;2009年30期

6 李墨;張國榮;;肺癌腦轉(zhuǎn)移瘤的治療進(jìn)展[J];疾病監(jiān)測與控制;2011年05期

7 徐西鳳;;伽瑪?shù)吨委?8例肺癌腦轉(zhuǎn)移瘤療效分析[J];中國醫(yī)療前沿;2012年16期

8 李陳;;不同類型肺癌腦轉(zhuǎn)移瘤的多層螺旋CT表現(xiàn)[J];臨床合理用藥雜志;2013年15期

9 宋正茂;;以首發(fā)神經(jīng)系統(tǒng)癥狀為特征的肺癌腦轉(zhuǎn)移瘤臨床治療體會[J];中國醫(yī)學(xué)創(chuàng)新;2013年12期

10 段傳志,徐如祥,李鐵林,陳長才,李新運;肺癌腦轉(zhuǎn)移瘤的治療[J];臨床醫(yī)學(xué);1995年03期

相關(guān)會議論文 前4條

1 姚世斌;李安民;張志文;傅相平;梁樹立;張宏達(dá);;肺癌腦轉(zhuǎn)移瘤的臨床治療體會(附38例報告)[A];中國醫(yī)師協(xié)會神經(jīng)外科醫(yī)師分會首屆全國代表大會論文匯編[C];2005年

2 劉原照;鄧湘;修霞;王德昌;李高峰;高鴻;褚德發(fā);;X刀在肺癌腦轉(zhuǎn)移瘤治療中的作用[A];2007第六屆全國放射腫瘤學(xué)學(xué)術(shù)年會論文集[C];2007年

3 錢偉;黃潤生;吳彩珍;毛樹權(quán);房景玉;;頭、體伽瑪?shù)堵?lián)合治療肺癌腦轉(zhuǎn)移瘤療效分析[A];2005年浙江省神經(jīng)外科學(xué)術(shù)會議論文匯編[C];2005年

4 孫文閣;石清磊;李煥煥;范國光;;半劑量三維容積T2Flair序列增強(qiáng)掃描在肺癌腦轉(zhuǎn)移瘤診斷中的應(yīng)用研究[A];2010中華醫(yī)學(xué)會影像技術(shù)分會第十八次全國學(xué)術(shù)大會論文集[C];2010年

相關(guān)碩士學(xué)位論文 前9條

1 張潔;肺癌腦轉(zhuǎn)移瘤中血管擬態(tài)現(xiàn)象與患者預(yù)后分析[D];蘇州大學(xué);2015年

2 楊翠翠;探討肺癌腦轉(zhuǎn)移瘤的MRI表現(xiàn)與原發(fā)灶病理類型的相關(guān)性[D];濟(jì)南大學(xué);2016年

3 鄢少聰;緊密連接相關(guān)蛋白在肺癌腦轉(zhuǎn)移瘤中的表達(dá)及臨床意義[D];南昌大學(xué);2016年

4 陸艷榮;肺癌腦轉(zhuǎn)移瘤立體定向放射治療療效與預(yù)后影響因素分析[D];新疆醫(yī)科大學(xué);2008年

5 蔡林波;肺癌腦轉(zhuǎn)移瘤不同放射治療方法的療效分析[D];南方醫(yī)科大學(xué);2007年

6 李文娟;肺癌腦轉(zhuǎn)移瘤的MRI表現(xiàn)與原發(fā)灶病理類型相關(guān)性的研究[D];吉林大學(xué);2013年

7 史宏志;磁共振彌散張量成像對肺癌腦轉(zhuǎn)移瘤放射治療療效評價的臨床意義[D];中國醫(yī)科大學(xué);2009年

8 侯麗娜;腫瘤標(biāo)志物聯(lián)合MRI檢查在小細(xì)胞肺癌腦轉(zhuǎn)移瘤中的應(yīng)用[D];山西醫(yī)科大學(xué);2013年

9 唐潔;DWI在肺癌腦轉(zhuǎn)移和腦膠質(zhì)瘤診斷和鑒別診斷中的應(yīng)用[D];昆明醫(yī)科大學(xué);2014年

，

本文編號：1416906