【摘要】：目的對濰坊地區(qū)噴泉水中檢測出的1株軍團(tuán)菌進(jìn)行分子生物學(xué)鑒定和系統(tǒng)發(fā)育分析,為濰坊市軍團(tuán)菌病的預(yù)防和控制提供有益信息。方法于2016年7~9月采集濰坊市不同水源的水樣,采用抽濾濃縮法濃縮獲得細(xì)菌濃縮液,用細(xì)菌基因組試劑盒提取濃縮菌液中的總細(xì)菌基因組DNA,使用針對軍團(tuán)菌屬16SrRNA基因片段的特異性引物PCR特異性擴(kuò)增軍團(tuán)菌屬16SrRNA基因片段并測序,與從GenBank獲得的另外31株軍團(tuán)菌的16SrRNA的基因序列進(jìn)行對比分析,采用MrBayesv3.2軟件對16SrRNA基因片段序列數(shù)據(jù)矩陣進(jìn)行貝葉斯分析并繪制基因進(jìn)化樹。結(jié)果 PCR擴(kuò)增濰坊市水樣軍團(tuán)菌屬特異性16SrRNA基因片段(386bp),其中一噴泉水樣陽性。測序分析該菌株(命名為WF-S)的16SrRNA基因序列與最新發(fā)現(xiàn)的沙特軍團(tuán)菌16SrRNA基因序列的遺傳距離較近,相似度達(dá)99.4%,在構(gòu)建的貝葉斯基因進(jìn)化樹中與沙特軍團(tuán)菌以其后驗(yàn)概率0.89聚類為一枝。結(jié)論 WF-S為一株沙特軍團(tuán)菌,在濰坊地區(qū)首次發(fā)現(xiàn),也是國內(nèi)首次報道的沙特軍團(tuán)菌。
[Abstract]:Objective to identify and systematically analyze a strain of Legionella detected in fountain water in Weifang area, so as to provide useful information for the prevention and control of Legionella in Weifang City. Methods the water samples from different water sources in Weifang City were collected from July to September 2016. The bacterial concentrated solution was obtained by filtration concentration method. The total bacterial genomic DNA, in the concentrated bacterial solution was extracted by bacterial genome kit. The 16SrRNA gene fragment of Legionella was amplified and sequenced by PCR, a specific primer for Legionella 16SrRNA gene fragment, and compared with the 16SrRNA gene sequence of 31 other strains of Legionella obtained from GenBank. The 16SrRNA gene fragment sequence data matrix was analyzed by MrBayesv3.2 software and the gene evolution tree was drawn. Results the specific 16SrRNA gene fragment (386bp) of Legionella in Weifang water sample was amplified by PCR, and one of the fountain water samples was positive. The genetic distance between the 16SrRNA gene sequence of this strain (named WF-S) and the newly discovered 16SrRNA gene sequence of Legionella Saudi was 99.4%. In the constructed Bayesian gene evolution tree, Legionella Saudi was clustered with its posterior probability of 0.89 as a branch. Conclusion WF-S is a strain of Legionella Saudi, which was found for the first time in Weifang area and is also the first reported in China.
【作者單位】： 濰坊醫(yī)學(xué)院醫(yī)學(xué)檢驗(yàn)學(xué)系濰坊醫(yī)學(xué)院醫(yī)學(xué)檢驗(yàn)學(xué)系納米醫(yī)學(xué)技術(shù)研究所濰坊醫(yī)學(xué)院臨床檢驗(yàn)診斷學(xué)山東省十二五高校重點(diǎn)實(shí)驗(yàn)室濰坊醫(yī)學(xué)院附屬醫(yī)院山東省臨床檢驗(yàn)重點(diǎn)專科;
【基金】：山東省自然科學(xué)基金項(xiàng)目(No.ZR2016HB70) 濰坊市科學(xué)技術(shù)發(fā)展計劃項(xiàng)目(No.2014WS046)
【分類號】：R126.4

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