【摘要】：目的探究紫山藥多糖(PYP)抗氧化活性與延長(zhǎng)壽命作用。方法將2 d齡雌性果蠅隨機(jī)分成4組,采用生存實(shí)驗(yàn)與急性氧化應(yīng)激損傷實(shí)驗(yàn)測(cè)定喂飼不同濃度(0、1.0、2.0、4.0 mg/ml)PYP對(duì)果蠅壽命的影響,并測(cè)定果蠅體內(nèi)抗氧化酶活力及抗氧化相關(guān)基因表達(dá)水平。結(jié)果添加2.0、4.0 mg/ml PYP可顯著延長(zhǎng)果蠅的平均壽命和最長(zhǎng)壽命,急性氧化應(yīng)激損傷實(shí)驗(yàn)中,各PYP組果蠅的壽命均明顯延長(zhǎng)。添加4.0 mg/ml PYP可顯著提高果蠅過氧化氫酶(CAT)、超氧化物歧化酶(SOD)活力,顯著降低丙二醛(MDA)含量,同時(shí),Mn-SOD、CAT、Cu,Zn-SOD m RNA表達(dá)水平顯著上調(diào),G蛋白偶聯(lián)受體基因(MTH)m RNA表達(dá)水平顯著下降。結(jié)論 PYP在體內(nèi)具有較強(qiáng)的抗氧化作用,并可能通過上調(diào)抗氧化基因的表達(dá)實(shí)現(xiàn)延長(zhǎng)果蠅壽命的作用。
[Abstract]:Objective to investigate the anti-oxidation activity and prolongation of life of (PYP). Methods two day old female Drosophila melanogaster were randomly divided into 4 groups. Survival test and acute oxidative stress injury test were used to determine the effects of different concentrations of PYP (1.0 ~ 2.0 mg/ml) on the lifespan of Drosophila melanogaster. The activity of antioxidant enzymes and the expression of antioxidant related genes in Drosophila melanogaster were determined. Results the average life span and longest life span of Drosophila melanogaster were significantly prolonged by adding 2.0 mg/ml PYP. In the experiment of acute oxidative stress injury, the life span of Drosophila melanogaster was significantly prolonged in all PYP groups. Addition of 4.0 mg/ml PYP significantly increased the (SOD) activity of (CAT), superoxide dismutase, decreased the content of malondialdehyde (MDA), and increased the expression of Mn-SOD,CAT,Cu,Zn-SOD m RNA in Drosophila melanogaster. The expression of G protein coupled receptor gene (MTH) m RNA decreased significantly. Conclusion PYP has strong antioxidation effect in vivo and may prolong the life span of Drosophila melanogaster by upregulating the expression of antioxidant gene.
【作者單位】： 河南農(nóng)業(yè)職業(yè)學(xué)院食品工程學(xué)院;
【基金】：鄭州市普通科技攻關(guān)項(xiàng)目(153PKJGG424) 2015年度河南省高等學(xué)校優(yōu)秀教學(xué)團(tuán)隊(duì)建設(shè)(河南農(nóng)業(yè)職業(yè)學(xué)院食品營(yíng)養(yǎng)與檢測(cè)專業(yè)) 2014年度河南省高等學(xué)�！皩I(yè)綜合改革試點(diǎn)”項(xiàng)目(河南農(nóng)業(yè)職業(yè)學(xué)院食品營(yíng)養(yǎng)與檢測(cè)專業(yè))
【分類號(hào)】：R151

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