【摘要】：L-阿拉伯糖是一種新興的功能性單糖,具有控制血糖升高、抑制脂肪生成、促進(jìn)雙歧桿菌生長等作用,如何快速高效的分離提取出高純度的L-阿拉伯糖已經(jīng)成為研究的熱點(diǎn)問題。目前工業(yè)上生產(chǎn)L-阿拉伯糖大多使用酸解半纖維素的方法,產(chǎn)物為多種單糖混合物。本實(shí)驗(yàn)針對(duì)目前樹脂或薄層色譜分離L-阿拉伯糖操作復(fù)雜且收效小的缺點(diǎn),對(duì)L-阿拉伯糖的微生物分離方法進(jìn)行研究,論文考察了不同種類微生物對(duì)玉米皮水解液中三種主要單糖,即葡萄糖、木糖和L-阿拉伯糖的利用情況,并取得了一定的成果。 文章首先對(duì)原材料和初提方法做了一個(gè)簡單的篩選,確定使用硫酸水解玉米皮制備出玉米皮酸水解液。利用高效液相色譜法確定了其中主要單糖的含量,葡萄糖、木糖和L-阿拉伯糖的含量分別占玉米皮干物質(zhì)的6.11%、18.82%和11.34%。并依此制備了模擬培養(yǎng)基。 之后通過對(duì)常見的5種酵母菌、7種霉菌、7種細(xì)菌、4種乳酸菌和兩株自壞境中分離篩選的霉菌Mgb1、Mgb2進(jìn)行模擬培養(yǎng)基的發(fā)酵,并使用高效液相色譜法對(duì)發(fā)酵期間的發(fā)酵液進(jìn)行檢測。結(jié)果表明所有菌株在1-2d內(nèi)都可將葡萄糖完全利用,細(xì)菌和乳酸菌不適用于對(duì)木糖和L-阿拉伯糖的分離。酵母菌中的球擬酵母,霉菌中的綠木霉、灰霉、Mgb1和Mgb2,對(duì)木糖和L-阿拉伯糖的利用具有較好的選擇性,在發(fā)酵3d之后木糖能夠被消耗掉絕大部分,剩余率僅為5.8%、2%、5.2%、4.5%和3.9%。而L-阿拉伯糖還有大量的保留,保留率為83.4%、70%、76.8%、88.6%和77.7%。 將模擬培養(yǎng)后篩選出的球擬酵母、綠木霉、灰霉、Mgb1、Mgb2五種菌株用于對(duì)玉米皮酸水解液的發(fā)酵并使用高效液相色譜法對(duì)發(fā)酵期間的發(fā)酵液進(jìn)行檢測。結(jié)果表明,球擬酵母發(fā)酵4d,其余4種菌株發(fā)酵3d以后木糖的剩余量均小于5%,而L-阿拉伯糖的保留率可達(dá)75%以上。分離方法簡單有效,所用的球擬酵母、綠木霉、灰霉3種菌株常見易得,為微生物法分離玉米皮水解液中的L-阿拉伯糖提供了一定的參考。
[Abstract]:L-arabinose is a new functional monosaccharide, which can control the increase of blood sugar, inhibit fat production and promote the growth of Bifidobacterium. How to extract high purity L-arabinose quickly and efficiently has become a hot issue. At present, L-arabinose is produced by acidolysis of hemicellulose. In this experiment, the microbiological separation method of L- arabinose was studied in allusion to the disadvantages of complex operation and low efficiency in the separation of L-arabinose by resin or thin-layer chromatography. In this paper, the utilization of three main monosaccharides, glucose, xylose and L-arabinose, in corn husk hydrolysate by different kinds of microorganisms was investigated, and some results were obtained. In this paper, a simple screening of raw materials and initial extraction methods was carried out, and the acid hydrolysate of corn husk was prepared by sulfuric acid hydrolysis. The main monosaccharides were determined by HPLC, and the contents of glucose, xylose and L-arabinose accounted for 6.1118.82% and 11.34% of the dry matter of corn skin, respectively. Based on this, the simulated medium was prepared. Then the fermentation medium was used to simulate the fermentation of 5 kinds of yeast, 7 kinds of mold, 7 kinds of bacteria, 4 kinds of lactic acid bacteria and 2 strains of fungi isolated from the bad environment. The fermentation broth during fermentation was detected by high performance liquid chromatography (HPLC). The results showed that all strains could make full use of glucose within 1-2 days, and bacteria and lactic acid bacteria were not suitable for the separation of xylose and L-arabinose. The utilization of xylose and L-arabinose by Mgb1 and Mgb2, was highly selective. After three days of fermentation, most of the xylose could be consumed, and the residual rate was only 5.8%. 2.5.2% and 3.9%. However, there are still a lot of reservations for L-arabinose, with a retention rate of 83.4% and 77.7%, 77.6% and 77.7%, respectively. Five strains, Trichoderma vulgaris, Trichoderma viride, Aspergillus graminearum and Mgb1,Mgb2, which were selected after simulated culture, were used to ferment the hydrolysate of corn peel acid and the fermentation broth during fermentation was detected by high performance liquid chromatography (HPLC). The results showed that the residual amount of xylose of the other four strains was less than 5 after 3 days fermentation, and the retention rate of L-arabinose could reach more than 75%. The isolation method is simple and effective, and the three strains of Trichoderma viride, Trichoderma viride and Trichoderma graminearum are common and easy to obtain, which provides a certain reference for the microbial separation of L-arabinose from corn husk hydrolysate.
【學(xué)位授予單位】：天津科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R151.2

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