大連沿海貝類產(chǎn)品副溶血性弧菌基因檢測(cè)
[Abstract]:Objective to investigate the contamination of Vibrio parahaemolyticus and the carrying of three virulence genes in shellfish products in Dalian, Liaoning Province. Methods 132 shellfish samples were collected from aquaculture farm and farmers' market around Dalian. The target gene tl gene of Vibrio parahaemolyticus was detected by real-time fluorescence PCR. The virulence genes of tdh,trh and orf8 were detected and analyzed. Results out of 132 shellfish samples, 43 were detected to carry target genes of Vibrio parahaemolyticus, the detection rate was 32.6% (43 / 132), of which 2 (4.7%) were positive for tdh gene (2 / 43). 2 trh gene positive (2 / 43), 1 orf8 gene positive (1 / 43), 2. 3% (1 / 43) tdh,orf8 gene positive, and 2. 3% (1 / 43) tdh, positive at the same time. Trh gene positive rate was 37.2% (16 / 43); The detection rate of target gene tl of Vibrio parahaemolyticus in oyster was 28.8% (23 / 80). Conclusion the situation of vibrio parahaemolyticus in shellfish of Dalian is serious.
【作者單位】: 大連市西崗區(qū)疾病預(yù)防控制中心檢驗(yàn)科;
【基金】:國(guó)家科技支撐計(jì)劃(2012BAK01B02)
【分類號(hào)】:R155.5
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