【摘要】：目的從污水處理系統(tǒng)中篩選強(qiáng)裂解能力的鼠傷寒沙門菌噬菌體,研究其生物學(xué)特性,為污水處理中病原菌的去除提供更好選擇。方法采用雙層平板法分離污水中的鼠傷寒沙門菌噬菌體,純化后測(cè)定其生物學(xué)特性。結(jié)果篩出的鼠傷寒沙門菌噬菌體Pst87170,其頭部為正二十面體立體對(duì)稱結(jié)構(gòu),直徑約87 nm,尾部較長(zhǎng)約為170 nm,直徑約9 nm,屬于ds DNA長(zhǎng)尾噬菌體科;最佳感染復(fù)數(shù)為0.1,潛伏期約100 min,爆發(fā)期約130 min,裂解量約103 PFU/cell。噬菌體Pst87170具有較強(qiáng)的裂解能力及良好的熱穩(wěn)定性和酸堿耐受性。結(jié)論噬菌體Pst87170具有研制成為噬菌體混合制劑而應(yīng)用于污水處理系統(tǒng)中去除相應(yīng)病原菌的潛力。
[Abstract]:Objective to screen the bacteriophage of Salmonella typhimurium from sewage treatment system and study its biological characteristics so as to provide a better choice for the removal of pathogenic bacteria in sewage treatment. Methods the bacteriophage of Salmonella typhimurium in sewage was isolated by double-layer plate method and its biological characteristics were determined after purification. Results the bacteriophage Pst87170, of Salmonella typhimurium showed an icosahedral stereomorphic structure on its head, and the length of 87 nm, tail was about 170 nm, about 9 nm, belonged to the family ds DNA. The best complex number of infection is 0.1, the latent period is about 100 min, outbreak period, about 130 min, cleavage quantity is about 103 PFU/cell.. Phage Pst87170 has strong pyrolysis ability, good thermal stability and acid-base tolerance. Conclusion Phage Pst87170 has the potential to be used as a bacteriophage mixture to remove pathogenic bacteria in wastewater treatment system.
【作者單位】： 太原理工大學(xué)環(huán)境科學(xué)與工程學(xué)院;
【基金】：山西省國(guó)際科技合作項(xiàng)目(2014081007-1)
【分類號(hào)】：R123;X703
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本文編號(hào)：2313486