【摘要】：目的研究蘋果多糖對小鼠結(jié)腸炎癌變的預(yù)防作用,并探討其作用機(jī)制。方法從蘋果渣中提取蘋果多糖,使用致炎劑葡聚糖硫酸鈉(DSS)和致癌劑氧化偶氮甲烷(AOM),建立小鼠結(jié)腸炎相關(guān)結(jié)直腸癌動物模型。通過免疫組織化學(xué)、蛋白質(zhì)印跡法、酶聯(lián)免疫分析等方法觀察蘋果多糖預(yù)防用藥對各組小鼠血清中促炎細(xì)胞因子腫瘤壞死因子(TNF)-α以及組織TLR4/My D88/NF-κB p65通路的影響。結(jié)果蘋果多糖預(yù)防用藥20周后,與模型組(95%致癌率)相比,蘋果多糖(1.25%、2.5%和5%)將結(jié)直腸腫瘤的發(fā)生率降至26%、10%、5%。蛋白質(zhì)印跡法結(jié)果顯示,蘋果多糖各個劑量組均降低結(jié)直腸組織中TLR4、My D88和NF-κB p65蛋白表達(dá)。酶聯(lián)免疫分析結(jié)果顯示,蘋果多糖各劑量組小鼠血清中腫瘤壞死因子-α水平明顯降低(P0.05)。結(jié)論蘋果多糖可有效預(yù)防結(jié)腸炎癌變,其作用機(jī)制可能與其抑制TLR4/My D88/NF-κB通路有關(guān)。
[Abstract]:Objective to study the preventive effect of apple polysaccharide on carcinogenesis of colitis in mice and its mechanism. Methods Apple polysaccharides were extracted from apple pomace, and the animal model of colitis associated colorectal cancer was established by using the inflammatory agent (DSS) and the carcinogen oxyazomethane (AOM),. Through immunohistochemistry, Western blotting, The effects of Apple Polysaccharide on the inflammatory cytokine (TNF)-偽 and TLR4/My D88 / NF- 魏 B p65 pathway in serum of mice were observed by enzyme-linked immunosorbent assay (Elisa). Results after 20 weeks, compared with the model group (95% carcinogenic rate), apple polysaccharide (1.25% and 5%) reduced the incidence of colorectal tumor to 2610% and 5%. The results of Western blot showed that the expression of TLR4,My D88 and NF- 魏 B p65 in colorectal tissues was decreased in all dosage groups of apple polysaccharides. The results of enzyme-linked immunosorbent assay (Elisa) showed that the levels of tumor necrosis factor- 偽 (TNF- 偽) in the serum of mice with different doses of apple polysaccharides were significantly decreased (P0.05). Conclusion Apple polysaccharides can effectively prevent colitis carcinogenesis, and its mechanism may be related to the inhibition of TLR4/My D88 / NF- 魏 B pathway.
【作者單位】： 西安醫(yī)學(xué)院病原生物學(xué)與免疫學(xué)教研室;第四軍醫(yī)大學(xué)藥理學(xué)教研室;陜西省友誼醫(yī)院藥劑科;西安醫(yī)學(xué)院藥理學(xué)教研室;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(81402063) 陜西省教育廳課題(15JK1636) 西安醫(yī)學(xué)院校級課題(2015D0009)
【分類號】：R151.3

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