【摘要】：目的監(jiān)測(cè)煙臺(tái)口岸淡色庫(kù)蚊攜帶黃病毒情況。方法在煙臺(tái)口岸采集淡色庫(kù)蚊,用RT-PCR方法檢測(cè)庫(kù)蚊黃病毒,采用MEGA 5.0軟件進(jìn)行遺傳距離計(jì)算和系統(tǒng)發(fā)育分析,確定該病毒的基因型。結(jié)果從煙臺(tái)口岸90組淡色庫(kù)蚊中的1組,擴(kuò)增到庫(kù)蚊黃病毒核酸,序列分析證實(shí)為庫(kù)蚊黃病毒。系統(tǒng)發(fā)育分析顯示與分離自山東東明的病毒株(JQ518484.1)遺傳關(guān)系最近,相似度達(dá)98%。該病毒分型為基因I型。結(jié)論煙臺(tái)口岸淡色庫(kù)蚊中檢測(cè)到庫(kù)蚊黃病毒,應(yīng)加強(qiáng)蚊蟲(chóng)攜帶病原體的監(jiān)測(cè)。
[Abstract]:Objective to monitor the yellow virus carried by Culex pipiens pallens at Yantai Port. Methods Culex pipiens pallens was collected at Yantai port. The yellow virus of Culex pipiens pallens was detected by RT-PCR method. Genetic distance calculation and phylogenetic analysis were carried out with MEGA 5.0 software to determine the genotype of the virus. Results the nucleic acid of Culex pipiens pallens was amplified from one of 90 groups of Culex pipiens pallens at Yantai Port. The nucleic acid of Culex pipiens was confirmed as Culex pipiens yellow virus by sequence analysis. Phylogenetic analysis showed that the genetic relationship between the virus strain (JQ518484.1) isolated from Dongming, Shandong Province, was the closest, and the similarity was 98%. The virus was classified as genotype I. Conclusion the yellow virus of Culex pipiens pallens was detected in Yantai Port.
【作者單位】： 煙臺(tái)出入境檢驗(yàn)檢疫局;山東出入境檢驗(yàn)檢疫局;
【基金】：國(guó)家質(zhì)檢總局科技計(jì)劃項(xiàng)目(2015IK200、2016IK313)
【分類號(hào)】：R185.3

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