【摘要】：目的：研究晝夜節(jié)律和就餐行為對(duì)于吸煙者及被動(dòng)暴露人群尿液可的寧濃度的影響,研究生活習(xí)性對(duì)于體內(nèi)尼古丁代謝速率的影響。 方法：選取32對(duì)夫婦,每組分別在晨間6-7點(diǎn)及下午5-6點(diǎn)集中于固定的面積為25m2的普通民居,男性吸煙者在1小時(shí)內(nèi)共吸煙4支,女性為被動(dòng)吸煙者,共同暴露1小時(shí),提取試驗(yàn)后1小時(shí)、6小時(shí)尿樣,加餐組在實(shí)驗(yàn)第4小時(shí)加入正常餐飲一次,最后尿樣以ELISA法測(cè)定各個(gè)時(shí)間點(diǎn)的Cotinine濃度,分析Cotinine整體的衰減趨勢(shì),日間與夜間的5小時(shí)范圍內(nèi)的Cotinine衰減幅度差異,未加餐與夾雜進(jìn)食行為的5小時(shí)范圍內(nèi)的Cotinine衰減幅度差異。 結(jié)果：32位男性7點(diǎn)樣本分別為757.4±207.8ng/ml、729.1±173.6ng/ml,13點(diǎn)樣本為562.8±162.0ng/ml、458.3±170.8ng/ml(加餐)、19點(diǎn)樣本為684.3±131.5ng/ml、647.8±205.2ng/ml,24點(diǎn)樣本為589.6±202.6ng/ml、484.2±143.8ng/ml(加餐)。所有組內(nèi)的2時(shí)間點(diǎn)Cotinine濃度進(jìn)行獨(dú)立T檢驗(yàn),P值均0.05,比較均值后發(fā)現(xiàn),隨時(shí)間都呈現(xiàn)衰減。晨間5小時(shí)衰減值均值為647.885ng/ml,標(biāo)準(zhǔn)差為205.26ng/ml,夜間5小時(shí)衰減值均值為484.254rig/ml,標(biāo)準(zhǔn)差為143.858ng/ml兩組數(shù)據(jù)進(jìn)行獨(dú)立T檢驗(yàn),方差不等,P0.05,有統(tǒng)計(jì)學(xué)差異。晨間未加餐5小時(shí)衰減值均值為194.6322ng/ml,標(biāo)準(zhǔn)差為54.406ng/ml,晨間加餐5小時(shí)衰減值均值為270.8161ng/ml,標(biāo)準(zhǔn)差為47.26ng/ml,兩組數(shù)據(jù)進(jìn)行獨(dú)立T檢驗(yàn),方差不等,P0.05,有統(tǒng)計(jì)學(xué)差異。夜間未加餐組5小時(shí)衰減值均值為94.719ng/ml,標(biāo)準(zhǔn)差為95.16342ng/ml,夜間加餐組5小時(shí)衰減值均值為163.631ng/ml,標(biāo)準(zhǔn)差為65.386ng/ml,兩組進(jìn)行獨(dú)立T檢驗(yàn),方差不等,P0.05,有統(tǒng)計(jì)學(xué)差異,夜間加餐組5小時(shí)衰減值夜間未加餐組5小時(shí)衰減值。 32位女性被動(dòng)吸煙者的濃度分別為7點(diǎn)：45.21±26.1ng/ml、45.8±14.7ng/ml、13點(diǎn):32.9±19.6ng/ml、31.7±21.4ng/m1(加餐)、19點(diǎn)：47.4±25.2ng/ml、38.5±21.0ng/ml、24點(diǎn)40.1±21.8ng/ml、24.2±5.3ng/ml(加餐)。未加餐晨間衰減值均值為38.559ng/ml,標(biāo)準(zhǔn)差為21.043ng/ml,未加餐夜間衰減值均值為24.276ng/ml,標(biāo)準(zhǔn)差為15.329ng/ml,兩組進(jìn)行獨(dú)立T檢驗(yàn),方差不相等,P0.05,有統(tǒng)計(jì)學(xué)差異,晨間未加餐衰減值未加餐夜間衰減值。晨間未加餐衰減值均值為12.2857ng/ml,標(biāo)準(zhǔn)差為7.333ng/ml,晨間加餐衰減值均值為14.0922ng/ml,標(biāo)準(zhǔn)差為9.745Ong/ml,兩組進(jìn)行獨(dú)立T檢驗(yàn),方差相等,P0.05,無(wú)統(tǒng)計(jì)學(xué)差異,晨間未加餐衰減值與晨間加餐衰減值無(wú)統(tǒng)計(jì)學(xué)差異。 結(jié)論：主動(dòng)吸煙者晨間吸煙可導(dǎo)致日間尼古丁代謝呈現(xiàn)較快水平,可影響日間體內(nèi)循環(huán)可的寧處于相對(duì)高水平,易導(dǎo)致對(duì)后續(xù)尼古丁的渴求行為增加。被動(dòng)吸煙者亦受晝夜節(jié)律影響,但不明顯,整體絕對(duì)值變化不大,可能系本身體內(nèi)循環(huán)可的寧水平較低有關(guān),進(jìn)食行為對(duì)于尼古丁代謝有促進(jìn)作用,短時(shí)間內(nèi)可加快Cotinine的代謝。
[Abstract]:Aim: to study the effects of circadian rhythm and eating behavior on the concentration of Karenin in urine of smokers and passive exposed people, and to study the effect of life habits on the metabolic rate of nicotine in vivo. Methods: 32 couples in each group were divided into two groups in the morning at 6: 7 and 5 to 6: 00 in the morning respectively. Male smokers smoked 4 cigarettes in one hour, and women were passive smokers who were exposed for 1 hour. The urine samples were extracted 1 hour and 6 hours after the experiment. The added meal group added normal food once in the 4th hour of the experiment. Finally, the concentration of Cotinine at each time point was measured by ELISA method, and the overall attenuation trend of Cotinine was analyzed. The range of Cotinine attenuation was different between daytime and night within 5 hours, and that between non-meal and inclusion eating within 5 hours. Results: in 32 males, 729.1 鹵173.6ng / ml of 729.1 鹵173.6ng / ml / min were 562.8 鹵162.0ng / ml / ml 458.3 鹵170.8ng/ml, and 684.3 鹵131.5ng / ml / ml 647.8 鹵205.2ngml / ml 24 points were 589.6 鹵202.6ng / ml / min and 589.6 鹵202.6ng / ml / min = 589.6 鹵202.6ng / ml / ml = 484.2 鹵143.8ng/ml, respectively. The Cotinine concentration in all groups was tested by independent T test at 2 time points, P value was 0.05. After comparing the mean value, it was found that all of them showed attenuation with time. The average attenuation value of 5 hours in the morning was 647.885ng / ml, the standard deviation was 205.26ng / ml, and the mean value of 5-hour attenuation value at night was 484.254rigp / ml. The standard deviation of the two groups of data was tested by independent T test, the variance was not equal to that of the two groups (P0.05, P 0.05). The average attenuation value of 5 hours in the morning was 194.6322 ng / ml, the standard deviation was 54.406 ng / ml, the average attenuation value of 5 hours was 270.8161 ng / ml, and the standard deviation was 47.26 ng / ml. The two groups of data were tested by independent T test, the variance was not equal to that of P0.05, there was statistical difference between the two groups. The mean value of 5 hours attenuation was 94.719 ng / ml and the standard deviation was 95.16342ng / ml in the nocturnal group, while the mean value of the 5-hour attenuation value was 163.631 ng / ml and the standard deviation was 65.386 ng / ml in the night plus meal group. The two groups were tested by independent T test, the variance was different (P0.05), there was statistical difference between the two groups. Attenuation value of 5 hours in nocturnal plus meal group. The concentration of 32 female passive smokers were 45.21 鹵26.1ngml / ml 45.8 鹵14.7 ng / ml 13 points: 32.9 鹵19.6nggml / ml / ml 31.7 鹵21.4ng/m1 respectively, 19:00: 47.4 鹵25.2ngml / ml 38.5 鹵21.0ngml / ml 24: 40.1 鹵21.8ngml-1 鹵24.2 鹵5.3ng/ml. The average attenuation value of non-meal morning was 38.559 ng / ml, the standard deviation was 21.043 ng / ml, the mean value of nocturnal attenuation was 24.276 ng / ml and the standard deviation was 15.329 ng / ml. The independent T test showed that the variance was not equal and the variance was not equal (P0.05), there was statistical difference between the two groups. The average attenuation value of non-meal in the morning was 12.2857ng / ml, the standard deviation was 7.333ng / ml, the average attenuation value of the morning meal was 14.0922ng / ml, and the standard deviation was 9.745Ong / ml. The two groups were tested by independent T test, the variance was equal, and there was no statistical difference between the two groups (P0.05). There was no statistical difference between the attenuation value of morning meal and that of morning meal. Conclusion: morning smoking in active smokers can lead to a faster level of day nicotine metabolism, which can affect the higher level of circulatory activity in the daytime, and may lead to an increase of nicotine craving behavior. Passive smokers were also influenced by circadian rhythm, but not obviously. The total absolute value was not changed significantly, which might be related to the lower level of Kokonin in the body. Eating behavior could promote nicotine metabolism and accelerate the metabolism of Cotinine in a short period of time.
【學(xué)位授予單位】：復(fù)旦大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R163

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