本文選題：塑化劑 + 鄰苯二甲酸二(-乙基己基)酯�。� 參考：《現(xiàn)代預(yù)防醫(yī)學》2015年06期

【摘要】：目的探討DEHP對中國倉鼠卵巢細胞(CHO細胞)的細胞毒性和凋亡基因癌基因表達水平的影響。方法不同劑量DEHP對CHO細胞進行染毒24 h和48 h,應(yīng)用CCK8試劑盒測定DEHP對CHO細胞的半數(shù)抑制濃度IC50,熒光定量PCR檢測凋亡基因(Bcl-2、caspase-8、caspase-9)和癌基因(c-fos、k-ras、p53)表達水平改變。結(jié)果DEHP染毒CHO細胞24 h的IC50為612μM,染毒48 h的IC50為336μM。DEHP較高劑量(150μM和300μM)染毒條件下,凋亡基因Bcl-2表達水平比對照組顯著降低,300μM DEHP染毒時caspase-8和caspase-9表達水平比對照組顯著增加;150μM和300μM DEHP染毒條件下c-fos表達水平增加;300μM DEHP染毒條件下k-ras基因表達水平升高;p53表達水平無明顯變化。結(jié)論 DEHP可通過激活caspase通路引起CHO細胞凋亡,也引起癌基因表達水平升高。
[Abstract]:Objective to investigate the effects of DEHP on cytotoxicity and expression of apoptotic gene oncogene in Chinese hamster ovarian cell (Cho). Methods CHO cells were exposed to different doses of DEHP for 24 h and 48 h. The IC50 concentration of DEHP on CHO cells was measured by CCK8 kit. The expression of apoptotic gene (Bcl-2caspase-8 caspase-9) and oncogene c-fosk-rasp53 were detected by fluorescence quantitative PCR. Results the IC50 of CHO cells exposed to DEHP for 24 h was 612 渭 M, and the IC50 of 48 h was 336 渭 M.DEHP. The expression of apoptosis gene Bcl-2 was significantly lower than that of control group. The expression level of caspase-8 and caspase-9 was significantly higher than that of control group under 300 渭 M DEHP exposure. The expression level of c-fos was significantly increased under the condition of 150 渭 M and 300 渭 M DEHP exposure. The expression level of k-ras gene was increased under the condition of 300 渭 M DEHP exposure. The expression level of k-ras gene was increased. There was no significant change in expression level. Conclusion DEHP can induce apoptosis of CHO cells and increase the expression of oncogene by activating caspase pathway.
【作者單位】： 廣東省深圳市疾病預(yù)防控制中心;深圳大學生命科學學院;
【分類號】：R114

【參考文獻】

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本文編號：1882104