本文選題：塑化劑 + 鄰苯二甲酸二(-乙基己基)酯��； 參考：《現(xiàn)代預(yù)防醫(yī)學(xué)》2015年06期

【摘要】：目的探討DEHP對(duì)中國(guó)倉(cāng)鼠卵巢細(xì)胞(CHO細(xì)胞)的細(xì)胞毒性和凋亡基因癌基因表達(dá)水平的影響。方法不同劑量DEHP對(duì)CHO細(xì)胞進(jìn)行染毒24 h和48 h,應(yīng)用CCK8試劑盒測(cè)定DEHP對(duì)CHO細(xì)胞的半數(shù)抑制濃度IC50,熒光定量PCR檢測(cè)凋亡基因(Bcl-2、caspase-8、caspase-9)和癌基因(c-fos、k-ras、p53)表達(dá)水平改變。結(jié)果DEHP染毒CHO細(xì)胞24 h的IC50為612μM,染毒48 h的IC50為336μM。DEHP較高劑量(150μM和300μM)染毒條件下,凋亡基因Bcl-2表達(dá)水平比對(duì)照組顯著降低,300μM DEHP染毒時(shí)caspase-8和caspase-9表達(dá)水平比對(duì)照組顯著增加;150μM和300μM DEHP染毒條件下c-fos表達(dá)水平增加;300μM DEHP染毒條件下k-ras基因表達(dá)水平升高;p53表達(dá)水平無(wú)明顯變化。結(jié)論 DEHP可通過(guò)激活caspase通路引起CHO細(xì)胞凋亡,也引起癌基因表達(dá)水平升高。
[Abstract]:Objective to investigate the effects of DEHP on cytotoxicity and expression of apoptotic gene oncogene in Chinese hamster ovarian cell (Cho). Methods CHO cells were exposed to different doses of DEHP for 24 h and 48 h. The IC50 concentration of DEHP on CHO cells was measured by CCK8 kit. The expression of apoptotic gene (Bcl-2caspase-8 caspase-9) and oncogene c-fosk-rasp53 were detected by fluorescence quantitative PCR. Results the IC50 of CHO cells exposed to DEHP for 24 h was 612 渭 M, and the IC50 of 48 h was 336 渭 M.DEHP. The expression of apoptosis gene Bcl-2 was significantly lower than that of control group. The expression level of caspase-8 and caspase-9 was significantly higher than that of control group under 300 渭 M DEHP exposure. The expression level of c-fos was significantly increased under the condition of 150 渭 M and 300 渭 M DEHP exposure. The expression level of k-ras gene was increased under the condition of 300 渭 M DEHP exposure. The expression level of k-ras gene was increased. There was no significant change in expression level. Conclusion DEHP can induce apoptosis of CHO cells and increase the expression of oncogene by activating caspase pathway.
【作者單位】： 廣東省深圳市疾病預(yù)防控制中心;深圳大學(xué)生命科學(xué)學(xué)院;
【分類(lèi)號(hào)】：R114

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 劉東堯;吳盛德;華q，

本文編號(hào)：1882104