本文選題：運(yùn)動 + 鐵狀態(tài)��； 參考：《廣州醫(yī)學(xué)院》2012年碩士論文

【摘要】：研究目的 通過給予不同鐵含量飲食以及長期大強(qiáng)度游泳運(yùn)動，觀察雄性大鼠血液鐵狀態(tài)、肝臟非血紅素鐵(NHI）含量、海馬NHI含量、海馬脂質(zhì)過氧化作用和羥自由基代謝的變化，探討飲食鐵含量和長期運(yùn)動對雄性大鼠外周及海馬鐵狀態(tài)的改變和意義。 研究方法 斷乳雄性SD大鼠90只，分為飲食低鐵含量組（12mg/kg），飲食標(biāo)準(zhǔn)鐵含量組（45mg/kg）和飲食高鐵含量組（1000mg/kg）。每組再分為運(yùn)動組和靜息組，即為六組，分別為低鐵含量飲食+運(yùn)動（EL）、低鐵含量飲食+靜息（SL）、標(biāo)準(zhǔn)鐵含量飲食+運(yùn)動（ES）、標(biāo)準(zhǔn)鐵含量飲食+靜息（SS）、高鐵含量飲食+運(yùn)動（EH）和高鐵含量飲食+靜息（SH）。各組用相應(yīng)的鐵含量飼料喂養(yǎng)1個月后，運(yùn)動組開始游泳，每周5天，每天1次，，持續(xù)3個月，靜息組除不進(jìn)行運(yùn)動外，其余處理同對應(yīng)運(yùn)動組。在最后一次運(yùn)動后，大鼠空腹24h，在戊巴比妥鈉麻醉下取靜脈血，測定紅細(xì)胞相關(guān)指標(biāo)，包括血紅蛋白（Hb）、紅細(xì)胞壓積（Hct）、平均紅細(xì)胞體積（MCV）、平均紅細(xì)胞血紅蛋白量（MCH）、紅細(xì)胞分布寬度（RDW）和血清鐵狀態(tài)指標(biāo)，包括血清鐵（SI）、血漿總鐵結(jié)合力（TIBC）并計算轉(zhuǎn)鐵蛋白飽和度（TS）。取肝臟、海馬組織液氮速凍，－80°C冰箱保存，測定肝臟、海馬NHI，海馬丙二醛含量（MDA）、抑制羥自由基（·OH）能力、超氧化物歧化酶活性（SOD）。 研究結(jié)果 1.飲食鐵含量和長期運(yùn)動對雄性大鼠外周鐵狀態(tài)的影響 飲食鐵含量對Hb、Hct、MCV、MCH、RDW、SI、TIBC、TS和肝臟NHI均有影響（P0.05），運(yùn)動對RDW、TIBC均有影響（P0.05）。 與SS組比較，SL組Hb、Hct、MCV、MCH、SI、TS和肝臟NHI均降低（P0.05），TIBC及RDW均顯著升高（P0.01）；SH組MCV降低（P0.05），肝臟NHI顯著升高（P0.01），Hb、Hct、MCH、RDW、SI、TIBC和TS均無差異（P0.05）。 與ES組比較，EL組Hb、Hct、MCV、MCH、SI、TS和肝臟NHI均降低（P0.05），TIBC及RDW均顯著升高（P0.01）；EH組肝臟NHI顯著升高（P0.01），Hb、Hct、MCV、MCH、RDW、SI、TIBC和TS均無差異（P0.05）。 與SL組比較，EL組TS降低（P0.05），TIBC顯著升高（P0.01），Hb、Hct、MCV、MCH、RDW、SI和肝臟NHI均無差異（P0.05）。 與SS組比較，ES組RDW升高（P0.05），Hb、Hct、MCV、MCH、SI、TIBC、TS和肝臟NHI均無差異（P0.05）。 與SH組比較，EH組肝臟NHI顯著降低（P0.01），RDW升高（P0.05），Hb、Hct、MCV、MCH、SI、TIBC和TS均無差異（P0.05）。 2.飲食鐵含量和長期運(yùn)動對雄性大鼠海馬鐵狀態(tài)、海馬脂質(zhì)過氧化作用、羥自由基代謝和SOD的影響 飲食鐵含量對海馬抑制羥自由基能力及SOD均有影響（P0.05），運(yùn)動對海馬MDA及抑制羥自由基能力均有影響（P0.05）。 與SS組比較，SL組海馬MDA及抑制羥自由基能力均降低（P0.05），海馬NHI及SOD均無差異（P0.05）；SH組海馬抑制羥自由基能力升高（P0.05），海馬NHI、MDA及SOD均無差異（P0.05）。 與ES組比較，EL組海馬NHI及抑制羥自由基能力均降低（P0.05），海馬MDA及SOD均無差異（P0.05）；EH組海馬抑制羥自由基能力及SOD均顯著升高（P0.01），海馬NHI及MDA均無差異（P0.05） 與SL組比較，EL組海馬NHI及抑制羥自由基能力均降低（P0.05），海馬MDA顯著升高（P0.01），海馬SOD無差異（P0.05） 與SS組比較，ES組海馬NHI、MDA、抑制羥自由基能力及SOD均無差異（P0.05）。 與SH組比較，EH組海馬NHI、MDA、抑制羥自由基能力及SOD均無差異（P0.05）。 研究結(jié)論 1.長期低鐵含量飲食會導(dǎo)致大鼠營養(yǎng)性鐵缺乏，肝臟貯存鐵含量減少；大鼠高鐵含量飲食時，Hb、血清鐵等血液學(xué)指標(biāo)均無變化，但肝臟內(nèi)貯存鐵增加，出現(xiàn)鐵過載。 2.在低鐵含量飲食時，長期運(yùn)動會加劇大鼠血清鐵狀態(tài)的降低，而肝臟貯存鐵含量無明顯變化，提示肝臟具有維持最低水平鐵貯存的機(jī)制；在鐵含量飲食足夠時，長期運(yùn)動會導(dǎo)致RDW升高，肝臟NHI沒有明顯變化，但有降低的趨勢；在高鐵含量飲食時，長期運(yùn)動會降低肝臟NHI含量，減輕肝臟鐵負(fù)荷。 3.飲食鐵含量與運(yùn)動單獨(dú)作用于雄性大鼠時，海馬NHI含量均沒有變化。在低鐵含量飲食時，長期運(yùn)動會加劇海馬NHI的降低；在飲食鐵含量充足及高鐵含量飲食時，運(yùn)動后海馬NHI無顯著變化。 4.長期低鐵含量飲食會增加海馬羥自由基水平，造成對海馬組織的自由基損傷；高鐵含量飲食時，海馬羥自由基水平明顯降低、SOD活力明顯升高，說明適度增大鐵含量的攝入有利于減少海馬組織的自由基損傷。在低鐵含量飲食時，長期運(yùn)動會升高海馬的羥自由基水平以及脂質(zhì)過氧化，加劇對海馬組織的過氧化損傷；在鐵含量飲食足夠或高鐵含量飲食時，長期運(yùn)動后海馬NHI、脂質(zhì)過氧化、羥自由基水平以及SOD均沒有變化。
[Abstract]:research objective
The iron status of the male rats, the content of the liver non heme iron (NHI), the NHI content of the hippocampus, the lipid peroxidation and the hydroxyl free radical metabolism in the hippocampus were observed by giving different iron content diet and long term large intensity swimming exercise. The changes and meaning of iron content and long-term movement of the diet on the peripheral and hippocampal iron status of the male rats were discussed. Righteousness.
research method
90 male SD rats were divided into diet low iron content group (12mg/kg), dietary standard iron content group (45mg/kg) and diet high iron content group (1000mg/kg). Each group was divided into exercise group and resting group, that is, six groups were low iron content diet + exercise (EL), low iron content diet + resting (SL), standard iron content diet + exercise (ES), and standard iron content. Diet + resting (SS), high iron content diet + exercise (EH) and high iron content diet + resting (SH). Each group was fed with the corresponding iron content feed for 1 months, the exercise group began to swim, 5 days a week, 1 times a day, lasting 3 months, rest in the rest of the same exercise group. After the last exercise, the rat fasting 24h, at amyl Venous blood was taken under barbiturate sodium anesthesia to determine red blood cell related indicators, including hemoglobin (Hb), red blood cell pressure (Hct), mean red cell volume (MCV), average red blood cell hemoglobin (MCH), red cell distribution width (RDW) and serum iron status, including serum iron (SI), plasma total iron junction (TIBC) and transferrin saturation (T) (T). S). Liver, hippocampal liquid nitrogen quick freezing, - 80 degree C refrigerator was preserved, liver, hippocampal NHI, hippocampal malondialdehyde content (MDA), hydroxyl free radical (. OH) ability and superoxide dismutase activity (SOD) were suppressed.
Research results
1. effects of dietary iron content and long-term exercise on peripheral iron status in male rats
Dietary iron content has effects on Hb, Hct, MCV, MCH, RDW, SI, TIBC, TS and liver NHI (P0.05). Exercise has an effect on RDW and NHI.
Compared with the SS group, SL group Hb, Hct, MCV, MCH, SI, TS and liver NHI all decreased (P0.05), TIBC and RDW were significantly higher.
Compared with the ES group, EL group Hb, Hct, MCV, MCH, SI, TS and liver NHI all decreased (P0.05), TIBC and RDW all increased significantly.
Compared with group SL, TS in group EL decreased (P0.05), TIBC increased significantly (P0.01), Hb, Hct, MCV, MCH, RDW, liver and liver had no difference.
Compared with group SS, RDW in group ES increased (P0.05), Hb, Hct, MCV, MCH, SI, TIBC, TS and liver were no difference.
Compared with group SH, the liver NHI of group EH was significantly decreased (P0.01), RDW increased (P0.05), Hb, Hct, MCV, MCH, SI, and MCV had no difference.
2. effects of dietary iron content and long-term exercise on hippocampal iron status, hippocampal lipid peroxidation, hydroxyl free Kidese and SOD in male rats
Iron content in diet affected hippocampal inhibition of hydroxyl free radical and SOD (P0.05). Exercise had an effect on hippocampal MDA and inhibition of hydroxyl radical (P0.05).
Compared with group SS, hippocampal MDA and hydroxyl free radical inhibition in SL group were decreased (P0.05), NHI and SOD in hippocampus were not different (P0.05), and hippocampal inhibition of hydroxyl radical ability in SH group (P0.05), hippocampus NHI, MDA and SOD were all no difference.
Compared with the ES group, the hippocampal NHI and the inhibition of hydroxyl radical in EL group were decreased (P0.05), MDA and SOD in hippocampus were not different (P0.05). The hippocampal hippocampal inhibition of hydroxyl radical and SOD increased significantly in EH group (P0.01), and there was no difference in hippocampal NHI and MDA.
Compared with group SL, the hippocampal NHI and hydroxyl free radical were decreased in group EL (P0.05), MDA in hippocampus increased significantly (P0.01), and there was no difference in hippocampus SOD (P0.05).
Compared with group SS, there was no difference in hippocampal NHI, MDA, hydroxyl free radical and SOD in group ES (P0.05).
Compared with group SH, there was no difference in hippocampal NHI, MDA, hydroxyl free radical and SOD in group EH (P0.05).
research conclusion
1. long term low iron content diet could lead to nutritional iron deficiency in rats and reduced iron content in liver. No changes in hematological indexes such as Hb and serum iron were found in rats with high iron content diet, but iron accumulation in liver was increased and iron overload appeared in the liver.
2. in the diet of low iron content, long-term exercise would aggravate the reduction of iron status in the serum of rats, but there was no obvious change in the liver storage iron content, suggesting that the liver has the mechanism to maintain the lowest level of iron storage. When the iron content is sufficient, the long-term exercise will lead to the increase of RDW, and the liver NHI has no obvious change, but there is a decreasing trend; in Gao Tiehan Long term exercise reduces liver NHI content and reduces liver iron burden.
3. the content of iron in diet and exercise alone in the male rats had no change in the hippocampal NHI content. In the low iron diet, long term exercise aggravated the decrease of NHI in the hippocampus, and there was no significant change in the sports Houhai horse NHI in the diet iron content and the high iron content diet.
4. long term low iron content diet increases hippocampal hydroxyl radical level and causes free radical damage to hippocampal tissue. When high iron content diet, the level of hippocampal hydroxyl radical is significantly reduced and SOD activity is significantly increased. It is suggested that the moderate increase in iron intake is beneficial to reduce the free radical damage of hippocampus tissue. It increased the hydroxyl radical level of the hippocampus and lipid peroxidation, aggravated the oxidative damage to the hippocampus, and had no changes in the long-term exercise of Houhai horse NHI, lipid peroxidation, hydroxyl radical level and SOD when the iron content diet was sufficient or high iron content diet.

【學(xué)位授予單位】：廣州醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R114

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