本文選題：鄰苯二甲酸二乙基己酯 + 學(xué)習(xí)��； 參考：《華中師范大學(xué)》2013年碩士論文

【摘要】：鄰苯二甲酸二乙基己酯(Di-(2-ethy lhexyl)phthalate, DEHP)作為一種人工合成有機(jī)化合物,以其良好的化學(xué)性質(zhì)和低廉的成本被廣泛用于塑料工業(yè)中,常被用作增塑劑添加到聚氯乙烯、聚乙稀、聚苯乙烯、聚丙烯塑料等塑料制品中,從而增加塑料制品的柔韌性和延展性、增強(qiáng)可塑性、提高塑料制品的強(qiáng)度。隨著使用時間的推移,它很容易從塑料中釋放,轉(zhuǎn)移至外環(huán)境,擴(kuò)散至空氣、土壤和水體中,造成對環(huán)境、生物、食品的污染。DEHP可以通過經(jīng)口、呼吸吸入、皮膚接觸、醫(yī)療暴露等途徑進(jìn)入人體。對于一般人群,從食物中吸收成為DEHP進(jìn)入人體的主要方式。DEHP具有多種生物毒性,包括遺傳毒性、神經(jīng)毒性、生殖毒性、免疫毒性和氧化損傷作用等。目前的研究項目中,對DEHP的生殖毒性的研究課題相對較多,而對神經(jīng)毒性研究比較少。 在本項研究的第一階段實驗中,選取雄性昆明小鼠作為研究對象,對其使用不同濃度的DEHP(0,5,50,500mg/kg)進(jìn)行連續(xù)10天的灌胃染毒暴露,通過Morris水迷宮對其學(xué)習(xí)與記憶能力進(jìn)行測試,并對腦組織中活性氧簇(Reactive oxygen species, ROS)和丙二醛(Malondialdehyde, MDA)含量進(jìn)行檢測,以判斷DEHP所造成的腦組織氧化損傷程度。隨后的第二階段實驗中,選用DEHP (50mg/kg)灌胃染毒同時添加維生素E (50mg/kg)作為保護(hù)劑,采用同樣的方法對小鼠的學(xué)習(xí)與記憶能力進(jìn)行檢測,同時測定小鼠腦組織中ROS與MDA的含量,來判斷維生素E對DEHP所產(chǎn)生的腦組織氧化損傷以及其引起的學(xué)習(xí)記憶能力減退的影響。第三階段實驗室關(guān)于小鼠情緒的研究,使用DEHP (500mg/kg)灌胃染毒暴露與氫化可的松陽性對照組相對比,分別通過開曠場實驗、懸尾實驗以及強(qiáng)迫游泳實驗,構(gòu)建抑郁模型,對小鼠的情緒以及抑郁程度進(jìn)行測定。 研究結(jié)果表明隨著DEHP暴露水平的增加,小鼠腦組織中的活性氧(ROS)和丙二醛(MDA)含量隨之增加,并且學(xué)習(xí)和記憶能力隨之降低；添加維生素E作為保護(hù)劑的小鼠腦組織氧化損傷損傷程度,以及由此造成的的學(xué)習(xí)和記憶能力減退有緩解；同時DEHP可能會導(dǎo)致小鼠的抑郁情緒。而導(dǎo)致其學(xué)習(xí)記憶能力減退級產(chǎn)生抑郁情緒的機(jī)制可能是由于DEHP所誘導(dǎo)的小鼠腦組織氧化應(yīng)激造成的。
[Abstract]:Diethylhexyl phthalate (DEHP), as a synthetic organic compound, is widely used in the plastics industry because of its good chemical properties and low cost, and is often used as a plasticizer for polyvinyl chloride (PVC), polystyrene (PS), polyvinyl chloride (PVC), polystyrene (PS), polyvinyl chloride (PVC), polyvinyl chloride (PVC) and polystyrene (PS). Polypropylene plastics and other plastic products, so as to increase the flexibility and ductility of plastic products, enhance plasticity, improve the strength of plastic products. And over time, it's very easy to release it from plastics, to move it out into the environment, to spread to the air, to the soil, to the water, to cause pollution to the environment, to living things, to food. DEHP can be inhaled through the mouth, breathed, and exposed to the skin. Medical exposure and other ways into the human body. For the general population, the main way to absorb DEHP from food into human body. DEHP has a variety of biological toxicity, including genotoxicity, neurotoxicity, reproductive toxicity, immune toxicity and oxidative damage and so on. Among the current research projects, there are more research topics on reproductive toxicity of DEHP, but less on neurotoxicity. In the first phase of this study, male Kunming mice were selected as the study subjects, and their learning and memory abilities were tested by Morris water maze. The contents of reactive oxygen specieses (Ross) and malondialdehyde malondialdehyde (MDAs) in brain tissue were determined to determine the degree of oxidative damage caused by DEHP. Then in the second phase of the experiment, the mice were given 50 mg / kg DEHP and 50 mg / kg vitamin E respectively as protective agent. The learning and memory abilities of mice were measured by the same method, and the contents of ROS and MDA in the brain tissue of mice were also measured. To determine the effects of vitamin E on brain oxidative damage and learning and memory impairment caused by DEHP. The emotion of mice was studied in the third stage of laboratory. The depression model was established by open field experiment, suspension test and forced swimming experiment, compared with hydrocortisone positive control group by intragastric administration of DEHP 500 mg / kg. The mood and depression of the mice were measured. The results showed that the contents of reactive oxygen species (Ros) and malondialdehyde (MDA) in brain tissue increased with the increase of DEHP exposure level, and the ability of learning and memory decreased. The degree of oxidative damage to brain tissue and the resulting impairment of learning and memory in mice treated with vitamin E as a protective agent were alleviated, and DEHP may lead to depression in mice. The mechanism of depression may be caused by oxidative stress in brain tissue induced by DEHP.
【學(xué)位授予單位】：華中師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R114

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