本文選題：納米材料 + 秀麗隱桿線蟲�。� 參考：《毒理學雜志》2015年06期

【摘要】：目的本研究對線蟲暴露于金納米團簇和納米碳點及其偶聯(lián)抗體產(chǎn)物(共4種醫(yī)用納米探針)后的感知行為和表型指標進行初步研究,以期探索應用線蟲評價納米材料安全性的新模式。方法將模式生物線蟲用于金納米團簇和納米碳點的毒性研究,分別觀察納米材料對線蟲的感知行為(趨避指數(shù)、趨向指數(shù))、線蟲表型指征(體型、灰度值、卷曲度、活動能力4個方面共16個指標)是否有改變,以判斷其毒性作用大小。結(jié)果金納米團簇(≤3.00 mg/ml)、偶聯(lián)抗體金納米團簇(≤3.00 mg/ml)、碳點(≤1.51 mg/ml)及偶聯(lián)抗體碳點(≤3.50 mg/ml)趨避實驗為陰性;在趨向試驗中,3.00、1.40和0.30 mg/ml金納米團簇、0.33 mg/ml碳點的趨向指數(shù)為陽性。16個非致死性指標總陽性率分別為偶聯(lián)抗體碳點(81%)偶聯(lián)抗體金納米團簇(69%)金納米團簇(56%)碳點(50%)。結(jié)論本研究初步探討了4種納米材料對線蟲感知行為的影響,且將線蟲的表型指征首次應用于納米材料毒性研究中,為探索納米材料的毒性提供了新穎的、有一定參考價值的快速篩選的指標。
[Abstract]:Objective to study the sensing behavior and phenotypic indexes of nematodes exposed to gold nanoclusters and carbon nanoparticles and their coupling antibody products (including four kinds of medical nanoprobes). In order to explore the application of nematodes to evaluate the safety of nanomaterials a new model. Methods the model biological nematode was used to study the toxicity of gold nanoclusters and carbon nanoparticles, and the perceived behavior of nanomaterials to nematodes (avoidance index, trend index, phenotypic indication of nematodes (shape, gray value, curvature) were observed, respectively. There were 16 indexes in 4 aspects of activity ability) to judge the toxicity. Results Gold nanoclusters (鈮，

本文編號：1819038