發(fā)布時間：2018-04-22 21:21

  本文選題：端粒體 + 端粒長度　； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:本研究旨在了解煤礦工人外周血端粒長度與原發(fā)性高血壓之間的關(guān)系,為煤炭工人預(yù)防高血壓提供科學(xué)依據(jù)。方法:1.使用QIAGEN公司TIA Namp Blood mini kit(51104)試劑盒提取測試對象外周血白細(xì)胞基因組DNA;2.使用實時熒光定量PCR測定端粒長度,T/S值為相對端粒長度,即端粒長度與單拷貝基因比值;3.運用SPSS19.0軟件對受試樣本進(jìn)行數(shù)據(jù)分析,研究其外周血端粒長度與高血壓的相關(guān)性。結(jié)果:1.本研究為配對設(shè)計實驗,高血壓病例組和對照組各230例。高血壓組平均收縮壓為145.73±16.99mm HG,平均舒張壓為91.87±14.37mm HG,對照組平均收縮壓為120.07±10.23mm HG,平均舒張壓為76.24±8.71mm HG,兩組存在顯著差異。2.高血壓病例組和對照組在年齡、身高方面無顯著差異;而在總膽固醇(TC)、谷氨酰轉(zhuǎn)肽酶(GGT)、谷氨酸(GLU)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、甘油三酯(TG)、總蛋白(TP)、BMI方面,差別具有統(tǒng)計學(xué)意義,高血壓病例組各指標(biāo)均高于對照組。3.高血壓病例組外周血端粒長度變化范圍是0.2149-1.9531,對照組外周血端粒長度的變化范圍為0.4562-2.1673,兩組相比高血壓組端粒長度明顯縮短(P0.001)。外周血白細(xì)胞端粒長度與年齡呈負(fù)相關(guān)(P0.05)。女性高血壓患者的端粒平均長度為1.2613±0.4112,男性高血壓患者端粒平均長度為0.9981±0.3965。男女高血壓患者端粒平均長度上存在顯著差異(P0.05)。結(jié)論:1.高血壓病例組在總膽固醇(TC)、谷氨酰轉(zhuǎn)肽酶(GGT)、谷氨酸(GLU)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、甘油三酯(TG)、總蛋白(TP)、BMI方面的指標(biāo)上高于對照組,差別具有統(tǒng)計學(xué)意義(P0.05)。2.端粒長度與年齡呈負(fù)相關(guān),年齡越大,端粒長度越短。男性高血壓端粒平均長度低于女性高血壓端粒平均長度。3.高血壓人群端粒長度與正常人群的端粒長度有差異,高血壓人群的相對端粒長度較正常人群短(P0.05)。
[Abstract]:Objective: to investigate the relationship between peripheral blood telomere length and essential hypertension in coal miners, and to provide scientific basis for preventing hypertension in coal miners. Method 1: 1. TIA Namp Blood mini kit (51104) of QIAGEN Company was used to extract genomic DNA from peripheral blood leukocytes. The T / S value of telomere length measured by real-time fluorescence quantitative PCR was relative telomere length, that is, the ratio of telomere length to single copy gene was 3. The correlation between peripheral blood telomere length and hypertension was studied by SPSS19.0 software. The result is 1: 1. This study was a pair design experiment, the hypertension case group and the control group 230 cases each. The mean systolic blood pressure (SBP) and diastolic blood pressure (DBP) were 145.73 鹵16.99mm and 91.87 鹵14.37mm in hypertension group, 120.07 鹵10.23mm and 76.24 鹵8.71mm in control group, respectively. There was significant difference between the two groups. There was no significant difference in age and height between the hypertension group and the control group, but there was no significant difference between the two groups in terms of total cholesterol, glutamyl transpeptidase (GGTN), glutamate GLU, high density lipoprotein (HDL), low density lipoprotein (LDLN), triglyceride (TGN), and total protein (TTP) BMI. The difference was statistically significant. The indexes of hypertension group were higher than that of control group. 3. 3. The range of telomere length in patients with hypertension was 0.2149-1.9531, and that in control group was 0.4562-2.1673.The telomere length in the two groups was significantly shorter than that in the hypertensive group (P 0.001). The telomere length of peripheral blood leukocytes was negatively correlated with age (P 0.05). The mean telomere length was 1.2613 鹵0.4112 in female hypertensive patients and 0.9981 鹵0.3965 in male hypertensive patients. There was significant difference in mean telomere length between male and female patients with hypertension (P 0.05). Conclusion 1. The indexes of TC, GGT, GLU, HDLX, LDLX, TGG, TGN, TG, TG, TG and TG in hypertension group were higher than those in control group, and the difference was statistically significant (P0.05. 2) in the hypertension group, there was no significant difference between the two groups in the index of total cholesterol, glutamyl transpeptidase, high density lipoprotein (HDL), low density lipoprotein (LDLN), triglyceride (TGN) and total protein (TGP). Telomere length was negatively correlated with age, and the older the telomere length was, the shorter the telomere length was. The mean telomere length of male hypertension is lower than that of female hypertension. The telomere length of hypertensive population was significantly different from that of normal population, and the relative telomere length of hypertensive group was shorter than that of normal population (P0.05).
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R135

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 景亞青;趙勇;王峰;;人細(xì)胞端粒DNA復(fù)制與長度維持[J];生命科學(xué);2014年11期

2 黃畢;楊艷敏;;端粒長度與心血管疾病關(guān)系的研究進(jìn)展[J];中國循環(huán)雜志;2013年07期

3 郭天姣;孫婉玲;張維;馬曉彩;劉聰艷;賀景娟;徐娟;;流式-熒光原位雜交技術(shù)在骨髓增生異常綜合征患者骨髓CD34~+細(xì)胞相對端粒長度檢測中的應(yīng)用[J];中國實驗血液學(xué)雜志;2013年05期

4 高凌云;李國棟;童坦君;;DNA印跡法和實時定量PCR法在端粒長度測定中的應(yīng)用比較[J];北京大學(xué)學(xué)報(醫(yī)學(xué)版);2013年02期

5 張衛(wèi)清;信雅菊;;煤礦工人高血壓狀況調(diào)查分析[J];中國療養(yǎng)醫(yī)學(xué);2011年12期

6 黃馨;石桂英;陳顯達(dá);鞠振宇;;利用流式熒光原位雜交法測定細(xì)胞端粒長度[J];中國比較醫(yī)學(xué)雜志;2010年07期

7 種冠峰;相有章;;中國高血壓病流行病學(xué)及影響因素研究進(jìn)展[J];中國公共衛(wèi)生;2010年03期

8 黃曉波;胡蓉;樂慶榮;侯永蘭;羅開良;胡淀;盧仙娥;殷躍輝;佘強;黃晶;;重慶市城鄉(xiāng)居民高血壓患病及影響因素分析[J];中國公共衛(wèi)生;2009年01期

9 郭令軍;張七一;;實時定量PCR分析外周血白細(xì)胞的端粒長度[J];中外醫(yī)療;2008年21期

10 郭曼;歐陽芳茜;王玉剛;;端粒和端粒酶與衰老研究[J];生命科學(xué)研究;2006年S3期

相關(guān)博士學(xué)位論文 前1條

1 黃馨;端�？s短影響原發(fā)性高血壓及其并發(fā)癥的發(fā)生[D];北京協(xié)和醫(yī)學(xué)院;2010年

相關(guān)碩士學(xué)位論文 前3條

1 汪金泉;外周血細(xì)胞染色體相對端粒長度與高血壓的相關(guān)性研究[D];石河子大學(xué);2014年

2 喬楠;同煤職工高血壓患病現(xiàn)狀及其影響因素分析[D];山西醫(yī)科大學(xué);2014年

3 劉娟;染色體端粒長度與肝癌發(fā)病及預(yù)后關(guān)系的實驗研究[D];第四軍醫(yī)大學(xué);2011年

，

本文編號：1789001