本文選題：驅(qū)蟲斑鳩菊注射　切入點(diǎn)：矽肺　出處：《新疆醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:檢測(cè)霧化吸入新疆維吾爾藥驅(qū)蟲斑鳩菊注射液(Vernonia Anthelmintica Willd Injection VAWI)導(dǎo)致矽肺患者血清蛋白的差異。方法:隨機(jī)選取2014年11月至2017年3月新疆醫(yī)科大學(xué)第五附屬醫(yī)院職防科矽肺壹期男性患者40例,按照1:1的比例將患者分為VAWI治療組和基礎(chǔ)治療組,各20例,另選取同期健康體檢者20例為正常組�；A(chǔ)治療組給予包括氧療、止咳、祛痰、平喘、抗炎等常規(guī)治療,VAWI治療組患者在基礎(chǔ)治療組的條件下加用驅(qū)蟲斑鳩菊注射液,2毫升VAWI加入到10毫升0.9%生理鹽水中進(jìn)行霧化吸入,兩組患者療程均為10-15天。抽取兩組患者治療前后與正常組的血液進(jìn)行離心,取血清,采用表面增強(qiáng)激光解吸離子化飛行質(zhì)譜(SELDI-TOF-MS)技術(shù)檢測(cè)三組之間血清蛋白的差異。結(jié)果:VAWI治療組、基礎(chǔ)治療組、正常對(duì)照組之間平均年齡、體重指數(shù)、文化程度、吸煙指數(shù)差異均無統(tǒng)計(jì)學(xué)意義(p0.05)。正常組與基礎(chǔ)治療組前預(yù)測(cè)蛋白名稱如下:complement c3 frag、amyloid beta a4 protein、hepcidin、fibrinogen alpha chain frag、neurosecretory protein vgf frag、alpha-1-antichymotrypsin frag、apolipoprotein c-i、transthyretin、apolipoprotein c-iii、apolipoprotein a-ii、cystatin-c。正常組與基礎(chǔ)治療組后預(yù)測(cè)蛋白名稱如下:amyloid beta a4 protein、hepcidin、fibrinogen alpha chain frag、neurosecretory、alpha-1-antichymotrypsin frag、apolipoprotein c-i、transthyretin、osteopontin frag、apolipoprotein c-iii、apolipoprotein a-ii、cystatin-c�；A(chǔ)治療組前與基礎(chǔ)治療組后預(yù)測(cè)蛋白名稱如下:amyloid beta a4 protein、plasma protease c1inhibitor frag、alpha-1-antichymotrypsin frag、neurosecretory protein vgf frag、fibrinogen alpha chain frag、osteopontin frag。VAWI治療組前與VAWI治療組后預(yù)測(cè)蛋白名稱如下:amyloid beta a4 protein、fibrinogen alpha chain frag、neutrophil defensin 1,neutrophil defensin 3、neurosecretory protein vgf frag、plasma protease c1 inhibitor frag、alpha-1-antichymotrypsin frag、apolipoprotein c-i、transthyretin、osteopontin frag、hemoglobin subunit beta、apolipoprotein a-ii、cystatin-c。血清蛋白的表達(dá)差異均有統(tǒng)計(jì)學(xué)意義(p0.05,閾值1)。結(jié)論:矽肺患者基礎(chǔ)治療前、后均與正常健康人群存在血清差異蛋白的表達(dá),矽肺患者進(jìn)行基礎(chǔ)治療可引起血清差異蛋白的表達(dá),驅(qū)蟲斑鳩菊注射液可導(dǎo)致矽肺患者血清差異蛋白的表達(dá)。
[Abstract]:Objective: to detect the difference of serum protein in silicosis patients induced by aerosol inhalation of Vernonia Anthelmintica Willd Injection VAWI. Methods: from November 2014 to March 2017, 5th affiliated Hospital of Xinjiang Medical University was randomly selected. A total of 40 male patients with silicosis in the Department of Vocational Protection, According to the ratio of 1: 1, the patients were divided into two groups: VAWI group (n = 20) and basic treatment group (n = 20). In the basic treatment group, 2 ml VAWI was added to 10 ml of normal saline in 10 ml of normal saline for atomization inhalation, and 2 ml of VAWI was added to 10 ml of normal saline. The course of treatment in both groups was 10-15 days. The blood samples from the two groups before and after treatment were centrifuged and serum samples were taken. Surface enhanced laser desorption ionization mass spectrometry (SELDI-TOF-MS) was used to detect the difference of serum protein among the three groups. There was no significant difference in smoking index between normal group and basic treatment group. The prepredicted protein names of normal group and basic treatment group were as follows: complement c3 fragamyloid beta a4 protein hepcidal fibrinogen alpha chain fragment secretory protein vgf fragment alpha-1-antichymotrypsin fragapolipoprotein c-itransretinopolipoprotein iiiapolipoprotein a-iapolipoprotein a-iapolipoprotein a-iapolipoprotein a iiapolipoprotein a iiapolipoprotein a iiapolipoprotein a iiapolipoprotein a iiapolipoprotein a iiapolipoprotein a cystatin-c.After the normal group and the basic treatment group, the prediction protein name was as follows: amyloid beta a4 hepcidin fibrinogen. Alpha chain fragmental neurosecretory alpha-1-antichymotrypsin apolipoprotein c-i transthyretinopontin / apolipoprotein c-iiiiiapolipoprotein a-iiapolipoprotein a-iiapolipoprotein a-iiolipoprotein cystatin-c. The names of the predictive proteins before and after treatment are as follows: amyloid beta a4 protein / protease C1fraginhibitor protease C1frag-secretory protein vgf fragmental protein vgf fragmental protein vgf fragmentogen alpha chain osteopontin frag.VAWI and VAWI treatment groups. The names of the predictive proteins before and after VAWI treatment are as follows: amyloid beta a4 protein alpha chain alpha defensin defensin defensin. 1neutrophil defensin 3 neurosecretory protein vgf fragment plasma protease C1 inhibitor fragment alpha-1-antichymotrypsin fragment apolipoprotein c-iopontin fragment protein subunit betaapolipoprotein a-iiapolipoprotein a-iidia cystatin-c.The difference of serum protein expression was significant (p0.05). Conclusion: before basic therapy for silicosis patients, there are significant differences in the expression of serum protein (p 0.05). After the treatment of silicosis patients, the expression of serum differential proteins could be induced by basic therapy, and the expression of serum differential proteins of silicosis patients could be induced by the injection of repellent turtledove chrysanthemum.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R135.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 付瑾;王貝貝;趙爽;董潔;冀嬌嬌;劉永剛;;民族藥驅(qū)蟲斑鳩菊中綠原酸的含量測(cè)定[J];中國(guó)處方藥;2015年10期

2 謝林峰;向俊蓓;孫亞男;徐曾濤;;蛋白質(zhì)芯片技術(shù)的臨床應(yīng)用新進(jìn)展[J];中國(guó)醫(yī)學(xué)創(chuàng)新;2015年23期

3 劉桂桃;高靜;哈木拉提·吾甫爾;努爾買買提;魏燕華;聶利平;金露;王小華;蔚文龍;;驅(qū)蟲斑鳩菊對(duì)大鼠實(shí)驗(yàn)性矽肺纖維化干預(yù)作用[J];中國(guó)職業(yè)醫(yī)學(xué);2015年01期

4 盧懿;胡建安;;含游離二氧化硅粉塵職業(yè)接觸限值的研究進(jìn)展[J];中華勞動(dòng)衛(wèi)生職業(yè)病雜志;2015年01期

5 楊晗;王飛;王全林;楊昆;;補(bǔ)陽還五湯對(duì)博萊霉素所致肺纖維化大鼠肺指數(shù)及肺組織病理的影響[J];中國(guó)實(shí)驗(yàn)方劑學(xué)雜志;2014年22期

6 彭海兵;曹福源;王建行;陳松;趙霞;劉燕;;槲皮素對(duì)硅肺纖維化大鼠肺組織TGF-β1、磷酸化P38MAPK表達(dá)及血清TNF-α水平的影響[J];鄭州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2014年05期

7 王文紅;熊御云;焦志軍;;液相芯片技術(shù)在臨床檢驗(yàn)中的應(yīng)用進(jìn)展[J];中華檢驗(yàn)醫(yī)學(xué)雜志;2014年08期

8 楊洋;任錦;劉晶;馬忠森;;DcR3在預(yù)防博萊霉素大鼠模型發(fā)生肺纖維化中的作用[J];中國(guó)老年學(xué)雜志;2014年09期

9 楊驍;袁援生;鐘華;;吡非尼酮抗纖維化作用在眼科的應(yīng)用[J];國(guó)際眼科雜志;2013年08期

10 藍(lán)蔚;劉德伍;彭燕;紀(jì)雪亮;易先鋒;;吡非尼酮對(duì)人增生性瘢痕成纖維細(xì)胞膠原蛋白分泌的影響[J];實(shí)用臨床醫(yī)學(xué);2013年04期

相關(guān)博士學(xué)位論文 前1條

1 李艷軍;彩色棉纖維比較蛋白質(zhì)組學(xué)研究及花色素還原酶GhANR基因的鑒定[D];石河子大學(xué);2013年

相關(guān)碩士學(xué)位論文 前4條

1 武珊珊;天津市某區(qū)矽塵危害動(dòng)態(tài)監(jiān)測(cè)與干預(yù)效果評(píng)估[D];天津醫(yī)科大學(xué);2014年

2 華正兵;運(yùn)動(dòng)為主的短期康復(fù)治療對(duì)大同煤礦煤工塵肺患者的療效[D];河北聯(lián)合大學(xué);2014年

3 陳慧;接矽塵作業(yè)人員體內(nèi)硅元素水平變化及與TGF-β1、TNF-α含量關(guān)系的研究[D];河北聯(lián)合大學(xué);2014年

4 白宇杰;雜交水稻鹽脅迫響應(yīng)差異蛋白質(zhì)組分析[D];遼寧師范大學(xué);2010年

，

本文編號(hào)：1637725