本文選題：果糖　切入點(diǎn)：血液指標(biāo)　出處：《湖南農(nóng)業(yè)大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

【摘要】：在過去的20年間,果糖作為甜味劑在食品工業(yè)中的使用量從20%增加至30%。隨著產(chǎn)量與用量迅速增長,人們在逐漸認(rèn)識(shí)到果糖獨(dú)特優(yōu)點(diǎn)的同時(shí),也開始關(guān)注果糖的營養(yǎng)特性與食用安全性。近年來果糖相關(guān)的營養(yǎng)學(xué)問題、食用安全性問題以及人體內(nèi)代謝途徑等方面的研究成為學(xué)界研究的熱點(diǎn)。本文用含10%、20%、30%果糖的飼料喂養(yǎng)28日齡的大鼠,以含10%葡萄糖的飼料和基礎(chǔ)飼料飼喂大鼠作參比,探討不同濃度的果糖飲食對大鼠機(jī)體健康的影響,得到以下結(jié)論： 1、每4d觀察記錄大鼠采食量、體重的變化,發(fā)現(xiàn)不同濃度的果糖和低濃度的葡萄糖飲食,未導(dǎo)致大鼠體重上升和肥胖,且果糖濃度與大鼠體重呈負(fù)相關(guān)關(guān)系,高濃度果糖飲食導(dǎo)致大鼠體重偏輕。 2、每14d檢測大鼠血清低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、膽固醇(CHO)、甘油三酯(TG)、瘦素(LP)、胰島素(INS)的含量,發(fā)現(xiàn)在大鼠未成年階段,果糖和葡萄糖對大鼠血清LDL,HDL, CHO,TG含量的影響較大。不同濃度的果糖,對大鼠的血液指標(biāo)的影響有所不同。當(dāng)?shù)蜐舛裙沁M(jìn)入機(jī)體后,使血清LDL、HDL、CHO及TG含量都升高,HDL和LDL的升高幅度沒有明顯差異。而高濃度果糖飲食刺激LDL和HDL含量上升同時(shí),HDL/LDL下降,且比低濃度果糖更大幅地使CHO含量上升,但對TG含量沒有明顯的影響。高低濃度的果糖飲食都能引發(fā)LP抵抗和INS升高,且低濃度果糖更明顯。 3、喂養(yǎng)至第56d時(shí),采集大鼠的肝臟樣本,稱重并檢測其中堿性磷酸酶(ALP)、硬脂酰輔酶A去飽和酶(SCD)、乙酰輔酶A羥化酶(ACC)、丙酮酸激酶(PK)和脂肪酸合成酶(FAS)的含量,發(fā)現(xiàn)高低濃度的果糖飲食都促進(jìn)肝臟脂質(zhì)的合成,且高濃度的果糖攝入導(dǎo)致肝臟病變。 試驗(yàn)發(fā)現(xiàn)不同濃度的果糖飲食能導(dǎo)致大鼠不同生理指標(biāo)的變化,影響生長發(fā)育。
[Abstract]:Over the past 20 years, the use of fructose as a sweetener in the food industry has increased from 20% to 30. Attention has also been paid to the nutritional characteristics and safety of fructose. In recent years, there have been nutritional problems related to fructose, The study on food safety and metabolic pathway in human body has become a hot topic in academic circles. In this paper, the rats of 28 days of age were fed with a diet containing 10 ~ 20% fructose and fed with a diet containing 10% glucose and a basic diet as a reference. To study the effects of fructose diet with different concentrations on the body health of rats, the following conclusions were obtained:. 1. The changes of intake and body weight of rats were observed every 4 days. It was found that different concentrations of fructose and low concentration of glucose diet did not cause weight gain and obesity in rats, and there was a negative correlation between fructose concentration and body weight of rats. High fructose diet resulted in low body weight in rats. 2. The contents of LDLX, HDLN, CHOX, TGN, LPX, INS were detected every 14 days in rats, and it was found that the contents of LDLX, HDLX, CHOX, TGN, LPX, INS) in rats were found to be in infancy. Fructose and glucose had a great effect on the contents of HDL and CHOG in serum of rats. Different concentrations of fructose had different effects on blood indexes of rats. When low concentrations of fructose entered the body, There was no significant difference between LDL and HDL-CHO and TG, but high fructose diet stimulated the increase of LDL and HDL content and decreased HDL / HDL content, and increased CHO content more than that of low fructose concentration. However, there was no obvious effect on TG content. Fructose diet with high or low concentration could induce LP resistance and INS increase, and low fructose concentration was more obvious. 3. At the 56th day of feeding, the liver samples of rats were collected, and the contents of alkaline phosphatase (ALPN), stearyl coenzyme A desaturase (SCD), acetyl coenzyme A hydroxylase (ACCK), pyruvate kinase (PKK) and fatty acid synthase (FASs) were measured. It was found that fructose diet promoted liver lipid synthesis, and high fructose intake led to liver lesion. It was found that different concentrations of fructose diet could lead to the changes of different physiological indexes and affect the growth and development of rats.
【學(xué)位授予單位】：湖南農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R151.2

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