發(fā)布時(shí)間：2018-02-28 00:29

  本文關(guān)鍵詞： Fe 氧化應(yīng)激 肺表面蛋白C　出處：《毒理學(xué)雜志》2015年01期 　論文類型：期刊論文

【摘要】：目的大氣細(xì)顆粒所含的過渡金屬元素與呼吸道疾病的發(fā)生密切相關(guān),但其誘導(dǎo)的氧化應(yīng)激對呼吸道細(xì)胞的影響仍不十分清楚。本文主要研究過渡金屬元素Fe誘導(dǎo)的肺泡上皮細(xì)胞氧化應(yīng)激對肺表面蛋白C(SP-C)表達(dá)的影響。方法肺泡II型上皮MLE-12細(xì)胞經(jīng)Fe暴露處理24 h后,使用噻唑藍(lán)(MTT)、2,7-二氯熒光素(DCF)和流式細(xì)胞測定細(xì)胞活力、細(xì)胞內(nèi)活性氧(ROS)生成和細(xì)胞凋亡;采用實(shí)時(shí)定量PCR和Western blot分析細(xì)胞的SP-C mRNA和蛋白表達(dá)水平變化。結(jié)果 Fe明顯抑制MLE-12細(xì)胞活力(P0.01),升高細(xì)胞內(nèi)的ROS生成水平(P0.01),并上調(diào)細(xì)胞SP-C mRNA和蛋白的表達(dá)水平。但其表達(dá)水平被過氧化氫酶、乙酰-L-半胱氨酸和磺酸去鐵敏預(yù)處理后明顯降低。結(jié)論 Fe誘導(dǎo)生成的ROS參與SP-C的表達(dá)。
[Abstract]:Objective the transition metal elements contained in fine particles of the atmosphere are closely related to the occurrence of respiratory diseases. However, the effect of oxidative stress on respiratory tract cells was still unclear. The effect of oxidative stress on the expression of lung surface protein (CnSP-C) in alveolar epithelial cells induced by transition metal element Fe was studied in this paper. The epithelial MLE-12 cells were exposed to Fe for 24 h. The cell viability, intracellular reactive oxygen species (Ros) production and apoptosis were measured by MTT and flow cytometry. Real time quantitative PCR and Western blot were used to analyze the expression of SP-C mRNA and protein in MLE-12 cells. Results Fe significantly inhibited the activity of MLE-12 cells, increased the level of ROS production and up-regulated the expression of SP-C mRNA and protein. Its expression level was expressed by catalase, After pretreatment with acetyl-L-cysteine and deferrite sulfonic acid, the expression of SP-C was significantly decreased. Conclusion Fe induced ROS is involved in the expression of SP-C.
【作者單位】： 中國科學(xué)院大學(xué)生命科學(xué)學(xué)院環(huán)境與健康研究室;中國農(nóng)業(yè)大學(xué)生物學(xué)院;
【基金】：國家自然科學(xué)基金委面上項(xiàng)目(21377127,11275264,U1432245,J1103520)
【分類號(hào)】：R114

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 曲凡;丁文軍;伊碩;張芳;;PM_(2.5)對肺泡Ⅱ型上皮細(xì)胞MLE-12的毒性研究[J];毒理學(xué)雜志;2010年01期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊宇軒;閆思琪;衣穎杰;張U，

本文編號(hào)：1544977