本文關(guān)鍵詞： 飲食熱能限制 腦老化 鐵調(diào)節(jié)蛋白- cAMP反應(yīng)元件結(jié)合蛋白　出處：《營養(yǎng)學(xué)報(bào)》2015年02期 　論文類型：期刊論文

【摘要】：目的以腦內(nèi)鐵調(diào)節(jié)蛋白-2(iron regulated protein-2,IRP-2)和c AMP反應(yīng)元件結(jié)合蛋白(c AMP response element binding protein,CREB)基因表達(dá)為觀測(cè)點(diǎn),探討飲食熱能限制(caloric restriction,CR)對(duì)小鼠學(xué)習(xí)記憶能力的影響。方法雄性小鼠40只隨機(jī)均分為正常對(duì)照組(normal control group,NC group)、CR組、腦老化組(brain aging group,BA group)、CR+BA組,每組10只,Morris水迷宮試驗(yàn)測(cè)定學(xué)習(xí)空間記憶能力,以逆轉(zhuǎn)錄聚合酶鏈反應(yīng)法(reverse transcription-polymerase chain reaction,RT-PCR)檢測(cè)小鼠大腦IRP-2及CREB基因的表達(dá)水平。結(jié)果與NC組相比,BA組逃避潛伏期(escape latency,EL)顯著增高(P0.05);CR+BA組的EL顯著低于BA組(P0.05),而與NC組無顯著差異(P0.05);CR組與NC組的EL相比無明顯差異(P0.05)。BA組IRP-2、CREB表達(dá)水平顯著低于NC組(P0.05);CR組IRP-2、CREB表達(dá)水平與NC組相比無明顯差異(P0.05);CR+BA組IRP-2、CREB表達(dá)水平顯著高于BA組(P0.05),與CR組及NC組相比無明顯差異(P0.05)。結(jié)論 CR可以上調(diào)BA組小鼠腦內(nèi)IRP-2及CREB基因表達(dá),提示CR能夠通過影響上述兩種基因的表達(dá)而增強(qiáng)BA組小鼠的學(xué)習(xí)記憶能力。NC小鼠給予CR后,其腦內(nèi)IRP-2及CREB基因的表達(dá)無明顯變化。
[Abstract]:Objective to observe the expression of iron-regulated protein -2iron regulated protein-2IRP-2 and c AMP response element binding protein in the brain. To investigate the effect of caloric restriction on the learning and memory ability of mice, 40 male mice were randomly divided into normal control group group group control group, brain aging group group BA group group and CR BA group. The ability of learning spatial memory was measured by Morris water maze test in 10 rats in each group. The expression of IRP-2 and CREB genes in the brain of mice was detected by reverse transcription-polymerase chain reaction- transcription-polymerase RT-PCR.Results compared with NC group, escape latencies of escape latencies in BALB group were significantly higher than those in control group, and the El of CR BA group was significantly lower than that in BA group, but had no significant difference with NC group. There was no significant difference in the expression of IRP-2CREB between the CR group and the NC group. The expression of IRP-2CREB in the P0.05- BA group was significantly lower than that in the NC group. There was no significant difference in the expression of IRP-2CREB between the CR group and the NC group. The expression level of IRP-2CREB in the CR group was significantly higher than that in the control group, and in comparison with the CR group and the NC group, the expression level of IRP-2CREB in the CR group was significantly higher than that in the control group. The expression of IRP-2CREB in the CR group was significantly higher than that in the NC group. Conclusion CR can up-regulate the expression of IRP-2 and CREB genes in the brain of BA group mice. These results suggest that CR can enhance the learning and memory ability of BA group mice by affecting the expression of these two genes. After CR, the expression of IRP-2 and CREB genes in the brain of the mice treated with CR has no obvious change.
【作者單位】： 山西農(nóng)業(yè)大學(xué)食品科學(xué)與工程學(xué)院;首都醫(yī)科大學(xué)實(shí)驗(yàn)動(dòng)物部;首都醫(yī)科大學(xué)公共衛(wèi)生與家庭醫(yī)學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(No.81373020) 北京市自然科學(xué)基金(No.7112014) 山西農(nóng)業(yè)大學(xué)科技創(chuàng)新基金(No.20132-02)
【分類號(hào)】：R151

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