發(fā)布時間：2018-02-21 04:32

  本文關(guān)鍵詞： 木漿源甾醇 倉鼠 膽固醇 腸道菌群　出處：《食品科學(xué)》2017年09期 　論文類型：期刊論文

【摘要】：以高脂高膽固醇膳食飼喂倉鼠為動物模型,研究木漿源甾醇對倉鼠膽固醇調(diào)控及腸道菌群的影響。45只實(shí)驗(yàn)動物隨機(jī)分成正常組、高脂組和3個不同劑量木漿源甾醇組(1‰、5‰、10‰)。測定血清中總膽固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)及高密度脂蛋白膽固醇(high density lipoprotein cholesterol,HDL-C)水平,應(yīng)用氣相色譜檢測糞便固醇排泄量,應(yīng)用實(shí)時熒光定量聚合酶鏈?zhǔn)椒磻?yīng)分析小腸中膽固醇調(diào)控相關(guān)基因的表達(dá)水平,應(yīng)用變性梯度凝膠電泳分析腸道菌群變化。結(jié)果顯示,與高脂組相比,給予木漿源甾醇倉鼠的TC、TG和non HDL-C水平均顯著降低(P0.05);5‰和10‰組倉鼠肝臟質(zhì)量極顯著降低(P0.01);木漿源甾醇5‰和10‰組乙酰輔酶A乙�；D(zhuǎn)移酶-2和微粒體甘油三酯轉(zhuǎn)運(yùn)蛋白mRNA表達(dá)水平均顯著降低(P0.05),10‰組羥甲基戊二酰輔酶A還原酶、三磷酸腺苷結(jié)合盒轉(zhuǎn)運(yùn)體G5和G8 mRNA表達(dá)水平顯著提高(P0.05);10‰組倉鼠中性固醇和酸性固醇排泄量均極顯著增加(P0.01);1‰和5‰劑量木漿源甾醇能顯著降低由高脂高膽固醇膳食引起的腸道菌群異常增殖(P0.05)。給予木漿源甾醇可顯著降低Oscillibacter sp.、Desulfovibrio sp.等菌的數(shù)量(P0.05),顯著增加Akkermansia sp.、Anaerostipes sp.及Roseburia sp.等菌的數(shù)量(P0.05)。結(jié)果表明,攝入木漿源甾醇可以增加倉鼠腸道膽固醇的排泄、抑制膽固醇的合成吸收和改善腸道菌群。
[Abstract]:The effects of sterol from wood pulp on cholesterol regulation and intestinal flora of hamsters were studied by feeding hamsters with high fat and high cholesterol diet. 45 experimental animals were randomly divided into normal group. Serum total cholesterol (TC), triglyceride triglyceride (TG) and high density lipoprotein cholesterol (HDL-C) were determined in high fat group and three different doses of wood pulp source sterol groups (1 鈥，

本文編號：1521002