本文關(guān)鍵詞： 蛹蟲草 子實體 類胡蘿卜素 提取 抗氧化活性　出處：《食品工業(yè)科技》2016年19期 　論文類型：期刊論文

【摘要】：在比較蛹蟲草子實體類胡蘿卜素的溶劑提取法、超聲波輔助提取法、微波輔助提取法、酸熱提取法的基礎(chǔ)上,對蛹蟲草子實體中類胡蘿卜素的提取工藝進行了優(yōu)化,并分析了類胡蘿卜素的體外抗氧化活性。實驗結(jié)果表明:酸熱提取法是幾種常見提取方法中類胡蘿卜素提取得率最高的一種方法;酸熱法最優(yōu)提取工藝為:鹽酸濃度為1 mol/L,鹽酸用量為20 m L/g,酸浸時間為30 min,熱處理時間為5 min,蛹蟲草中類胡蘿卜素的得率達到1387μg/g�？寡趸瘜嶒灲Y(jié)果顯示:蛹蟲草子實體中類胡蘿卜素具有較好的體外抗氧化活性,在0~10 mg/m L的濃度范圍內(nèi)抗氧化能力隨著類胡蘿卜素濃度的提高而增加。4 mg/m L類胡蘿卜素的DPPH自由基清除率達到96.57%,類胡蘿卜素對鐵離子螯合能力的EC50值為3.26 mg/m L,且還顯示出了較好的還原能力。
[Abstract]:On the basis of comparing the solvent extraction, ultrasonic assisted extraction, microwave assisted extraction and acid heat extraction of carotenoid from Cordyceps militaris fruiting body, the extraction process of carotenoid from fruiting body of Cordyceps militaris was optimized. The antioxidant activity of carotenoid in vitro was analyzed. The results showed that the acid heat extraction method was the most effective method for extracting carotenoid. The optimum extraction process by acid-heat method was as follows: concentration of hydrochloric acid was 1 mol / L, dosage of hydrochloric acid was 20 mL / g, acid leaching time was 30 min, heat treatment time was 5 min, and the yield of carotenoid from Cordyceps militaris was 1387 渭 g / g. The results of antioxidant experiment showed that: Cordyceps militaris L. Carotenoids have good antioxidant activity in vitro. In the range of 0 ~ 10 mg/m / L, the antioxidant activity increased with the increase of carotenoid concentration. The DPPH free radical scavenging rate of carotenoid reached 96.57. The EC50 value of carotenoid chelating ability to iron ion was 3.26 mg/m / L. It also shows good reduction ability.
【作者單位】： 江蘇省食品資源開發(fā)與質(zhì)量安全重點建設(shè)實驗室徐州工程學(xué)院;
【基金】：江蘇省科技計劃項目(BN2015012) 江蘇省高校自然科學(xué)研究重大專項(15KJA180008)聯(lián)合資助
【分類號】：R151;TS264.4
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本文編號：1501909