本文關(guān)鍵詞： 賴氨酸 穩(wěn)定同位素 豐度 ~(13)CO_2產(chǎn)生率 氧化率 二項回歸曲線　出處：《揚州大學》2012年碩士論文　論文類型：學位論文

【摘要】：1研究背景 我國民眾以谷物膳食為主,蛋自質(zhì)攝入以植物性來源為主,曾有某省的人群營養(yǎng)調(diào)查結(jié)果顯示高達90%以上氨基酸模式不理想,其中第一限制性的氨基酸就是賴氨酸。賴氨酸是人體必需氨基酸之一、谷物的第一限制性氨基酸,明確其人體需要量對以谷類為主食的中國居民具有頗為重要的意義。缺乏賴氨酸的癥狀包括疲勞、虛弱、惡心、嘔吐、頭暈、沒有食欲、發(fā)育遲緩、貧血.等。在谷類食品中強化賴氨酸以提高蛋白質(zhì)營養(yǎng)價值的研究,國內(nèi)外早有報道。Mack早在20世紀中葉就發(fā)現(xiàn),在基礎膳食中補充賴氨酸喂養(yǎng)兒童,體重增加及骨質(zhì)密度增強等均優(yōu)于未補充者,這說明賴氨酸可促進生長和骨鈣沉著。然而任何營養(yǎng)素攝入都應該遵循適量的原則,也有許多研究報道了攝入過量賴氨酸引起的有害作用。目前我國尚未制定出適合中困居民膳食特點的賴氨酸推薦攝入量,相關(guān)研究也較少,及早制定出適合中國人群的必需氨基酸推薦攝入量的意義非常重大。本實驗采用先進安全的指示劑氨基酸氧化法(13C-苯丙氨酸作為指示劑)來研究中國健康青年男性的賴氨酸生理需要量。 2研究目的 本研究擬以中國當前的膳食模式下的健康成年男性作為研究對象,采用穩(wěn)定同位素示蹤技術(shù),以13C-苯丙氨酸作為示蹤劑,結(jié)合“2002年年中國居民營養(yǎng)與健康狀況調(diào)查”資料所得出的中國居民代表性膳食結(jié)構(gòu),探討我國健康成年男性的賴氨酸需要量,為制定我國不同性別、人群的必需氨基酸推薦攝入量提供科學依據(jù),為我國的食品氨基酸強化和臨床的氨基酸治療提供理論支持。同時,將本研究結(jié)果與西方人種的結(jié)果進行比較,探討不同人種對賴氨酸需要最的異同及其原因,為進步探索人體對氨基酸代謝的機制提供實驗數(shù)據(jù)。 3研究方法 木研究以中國當前的膳食模式的健康青年男性作為研究對象,預設5個賴氨酸攝入水平：65mg/(kg·d),55mg/(kg·d),45mg/(kg·d),35mg/(kg·d),25mg/(kg·d)。分為5個連續(xù)的實驗周期,侮周為期7天。選擇7名健康青年男性作為研究對象,每周1～6天為適應期,周日進行氨基酸代謝動力學研究。采用穩(wěn)定同位素示蹤技術(shù),以13C-苯丙氨酸作為示蹤劑進行人體氨基酸代謝實驗,檢測呼出氣、血液中穩(wěn)定同位素的豐度,計算氨笨酸的流量.和氧化率,通過觀察比較在不同膳食賴氨酸攝入水平下,5個賴氨酸劑景組的苯丙氨酸氧化情況的變化規(guī)律來了解機體賴氨酸的氧化分解情況,建立賴氨酸攝入量與苯丙氨酸氧化率變化之間的二項回歸曲線,計算回歸曲線的拐點來確定賴氨酸的生理需要量。 4研究結(jié)果 13CO2產(chǎn)生率計算的賴氨酸生理需要量為57.08mg/(kg·d) 13C-苯內(nèi)氨酸的氧化率計算的賴氨酸生理需要量53.46mg/(kg·d) 5結(jié)論 在中國傳統(tǒng)膳食模式卜,中國健康青年男性賴氨酸的生理需要量范圍在50～60mg/(kg·d)。
[Abstract]:1 research background Most of the people in our country mainly eat cereals, and the natural intake of eggs is mainly from vegetative sources. The results of nutritional survey in one province showed that the pattern of amino acid was not satisfactory as high as 90% or more. The first limiting amino acid is lysine. Lysine is one of the essential amino acids in human body and the first limiting amino acid in cereals. Identifying their human needs is of great significance to Chinese residents who rely on cereals. Symptoms of lysine deficiency include fatigue, weakness, nausea, vomiting, dizziness, loss of appetite, and stunting. Anemia, et al. Studies on fortifying lysine in cereals to improve protein nutritional value have long been reported at home and abroad. Mack was found in the middle of 20th century to supplement lysine in basic diet to feed children. Weight gain and bone density were better than those without supplementation, suggesting that lysine promoted growth and osteocalcium. However, any nutrient intake should follow appropriate principles. There are also many studies reported on the harmful effects of excessive intake of lysine. At present, China has not yet developed a suitable dietary characteristics of the poor residents of lysine recommended intake, and there are few related studies. It is very important to determine the recommended intake of essential amino acids suitable for Chinese population as soon as possible. The advanced safe indicator amino acid oxidation method (13C- phenylalanine) was used as indicator in this experiment. To study the lysine physiological requirements of healthy young men in China. 2 purpose of research In this study, healthy adult males in the current dietary pattern in China were chosen as study objects, and 13C- phenylalanine was used as tracer by using stable isotope tracer technique. Based on the data of the 2002 Survey of Nutrition and Health status of Chinese residents, the lysine requirements of healthy adult males in China were discussed in order to formulate different sexes in China. The recommended intake of essential amino acids in the population provides a scientific basis for the strengthening of food amino acids and the clinical treatment of amino acids in China. At the same time, the results of this study are compared with the results of Western ethnic groups. To explore the most similarities and differences of lysine requirement among different species and its causes, and to provide experimental data for improving the mechanism of amino acid metabolism in human body. 3 research methods In this study, healthy young men in the current dietary pattern in China were selected as subjects, with 5 lysine intake levels of 5: 65 mg / kg / kg / d = 55 mg / kg 路d). 45 mg / kg / d ~ 35 mg 路kg 路d ~ (-1) ~ (25 mg 路d ~ (-1) ~ (-1) / kg / d) were divided into five consecutive experimental cycles. Seven healthy young men were selected as the study subjects. The amino acid metabolism kinetics was studied on Sunday. Stable isotope tracer technique was used to study the amino acid metabolism kinetics of 7 healthy young men during the adaptation period of 1 to 6 days a week. Amino acid metabolism experiments were carried out with 13C- phenylalanine as tracer to detect the abundance of stable isotopes in exhaled air and blood, and to calculate the flow rate and oxidation rate of aminobenzoic acid. In order to understand the oxidative decomposition of lysine, the changes of phenylalanine oxidation in five lysine groups were observed and compared under different dietary lysine intake levels. A binomial regression curve between lysine intake and phenylalanine oxidation rate was established and the inflection point of the regression curve was calculated to determine the physiological requirement of lysine. 4. Research results The Lysine physiological requirement calculated by 13CO2 production rate is 57.08 mg / kg 路d) The Lysine physiological requirement calculated by the Oxidation rate of 13C- phenylalanine: 53.46 mg / kg 路d) Conclusion In the traditional Chinese dietary pattern, the physiological requirement of lysine for healthy young Chinese males is in the range of 50 ~ 60 mg / kg 路d ~ (-1) 路d ~ (-1).
【學位授予單位】：揚州大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R151.1

【參考文獻】

相關(guān)期刊論文 前6條

1 蔡傳江;王立賢;趙克斌;車向榮;顏華;程篤學;喬莉娟;;降低日糧賴氨酸凈能比對育肥豬生產(chǎn)性能及肉品質(zhì)的影響[J];動物營養(yǎng)學報;2010年04期

2 黃健;鄧紅;姚焰礎;;氨基酸水平對種肉兔生產(chǎn)性能的影響研究[J];飼料工業(yè);2010年19期

3 謝建兵;H.C.Lee;段紅偉;于鋒;;飼糧不同賴氨酸水平對早期斷奶仔豬生產(chǎn)性能的影響[J];飼料工業(yè);2010年20期

4 唐愛國;高效液相色譜法快速直接測定血清苯丙氨酸和酪氨酸[J];湖南醫(yī)科大學學報;2000年02期

5 田穎;樸建華;勾凌燕;田園;李衛(wèi)東;楊曉光;;進食狀態(tài)下口服~(13)C-亮氨酸呼出氣平臺期研究[J];衛(wèi)生研究;2008年02期

6 田穎;楊曉光;樸建華;;穩(wěn)定同位素技術(shù)在組織蛋白質(zhì)代謝研究中的應用[J];衛(wèi)生研究;2008年01期

相關(guān)博士學位論文 前1條

1 田穎;中國青年女性膳食蛋白質(zhì)參考攝入量研究[D];中國疾病預防控制中心;2008年

，

本文編號：1478829