本文選題：-甲氧基雌二醇 + 細(xì)胞膜色譜�。� 參考：《鄭州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年02期

【摘要】：目的:探討實(shí)驗(yàn)室合成的2-甲氧基雌二醇(2-ME)及其合成中間體的大鼠肺細(xì)胞膜色譜行為。方法:經(jīng)篩選,大鼠細(xì)胞膜色譜條件為自制大鼠肺細(xì)胞膜色譜柱(10 mm×2 mm),流動(dòng)相為10 mmol/L磷酸鹽緩沖液(p H7.4),柱溫為37℃,流速為0.2 m L/min。以沙丁胺醇為陽性對(duì)照,以容量因子k'作為指標(biāo),考察2-ME及其合成中間體在大鼠肺細(xì)胞膜色譜柱上的保留特性。結(jié)果:2-ME及合成中間體1、2號(hào)化合物在大鼠肺細(xì)胞膜色譜柱上均有吸附,且k'依次為2號(hào)化合物1號(hào)化合物2-ME;合成中間體3及4號(hào)化合物未表現(xiàn)出吸附特性。2-ME與大鼠肺細(xì)胞膜色譜柱的親和力大于純硅膠色譜柱、大鼠心肌細(xì)胞膜色譜柱和肝細(xì)胞膜色譜柱。結(jié)論:2-ME在大鼠肺細(xì)胞膜上具有特異性親和。
[Abstract]:Aim: to investigate the chromatographic behavior of 2-methoxyestradiol (2-ME2) and its intermediate in rat lung cell membrane.Methods: the chromatographic conditions of rat cell membrane were as follows: the chromatographic column of rat lung cell membrane was 10 mm 脳 2 mm, the mobile phase was 10 mmol/L phosphate buffer solution (pH 7.4), the column temperature was 37 鈩，

本文編號(hào)：1758150