【摘要】：目的觀察糖尿病小鼠角膜形態(tài)損害的特點(diǎn),為后期進(jìn)行的糖尿病角膜病變保護(hù)因子研究提供研究基礎(chǔ)。方法 STZ誘導(dǎo)的糖尿病C57BL/J小鼠,分別于糖尿病成功造模后3個(gè)月、6個(gè)月時(shí),選取糖尿病小鼠和正常對(duì)照組小鼠角膜,石蠟包埋后行HE染色觀察形態(tài)學(xué)變化。結(jié)果糖尿病成功造模后3個(gè)月、6個(gè)月,與正常對(duì)照組相比,糖尿病小鼠角膜水腫、基質(zhì)增厚,角膜潰瘍區(qū)上皮缺損,潰瘍區(qū)基質(zhì)增厚、角膜水腫明顯高于非潰瘍區(qū)。結(jié)論糖尿病小鼠造模后3個(gè)月即可發(fā)生角膜基質(zhì)病變,6個(gè)月時(shí)角膜基質(zhì)改變更加嚴(yán)重。
[Abstract]:Objective to observe the characteristics of corneal morphological damage in diabetic mice and to provide a basis for the study of protective factors of diabetic keratopathy in the later stage. Methods Diabetic C57BL/J mice induced by STZ were selected from the cornea of diabetic mice and normal control mice at 3 and 6 months after successful modeling of diabetes mellitus, and the morphological changes were observed by HE staining after paraffin embedding. Results compared with the normal control group, corneal edema, matrix thickening, epithelial defect in corneal ulcer area, matrix thickening and corneal edema in diabetic mice were significantly higher than those in non-ulcer area at 3 months and 6 months after successful establishment of diabetic model. Conclusion Corneal stroma lesions can occur in diabetic mice at 3 months after modeling, and the corneal stroma changes are more serious at 6 months.
【作者單位】： 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬同濟(jì)醫(yī)院眼屈光治療中心;
【基金】：國(guó)家自然科學(xué)基金資助(編號(hào):81271015) 湖北省自然科學(xué)基金資助(編號(hào):2009CDB206)~~
【分類號(hào)】：R587.2;R772.2

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