【摘要】：目的研究趨化因子受體7(chemokine receptor 7,CCR7)基因重組腺病毒體外轉(zhuǎn)染供體骨髓來源的未成熟樹突狀細(xì)胞(immature dendritic cells,imDC)對(duì)大鼠高危角膜移植免疫排斥反應(yīng)的作用。方法以60只SD大鼠為受體,30只Wistar大鼠為供體,堿燒傷建立高危角膜移植模型。將SD大鼠分為空白對(duì)照組、未修飾imDC組、imDC+腺病毒空載體組(imDC+Ad組)和imDC+CCR7腺病毒組(imDCs+Ad-CCR7組),每組15只;各組受體術(shù)前7 d和術(shù)后3 d經(jīng)尾靜脈注入PBS液0.1 mL、供體來源的未修飾imDC 10×106個(gè)(0.1mL)、空載體腺病毒轉(zhuǎn)染后的imDC 10×106個(gè)(0.1 mL)和攜帶CCR7腺病毒轉(zhuǎn)染后的imDC 10×106個(gè)(0.1 mL);術(shù)后受體大鼠每天裂隙燈下觀察角膜植片存活情況并對(duì)角膜植片的混濁、水腫及新生血管程度進(jìn)行評(píng)分;術(shù)后14 d每組隨機(jī)處死5只大鼠取角膜行HE切片觀察。結(jié)果角膜植片存活時(shí)間:空白對(duì)照組(10.44±1.88)d,imDC組(16.00±2.18)d,imDC+Ad組(15.11±2.03)d,imDC+Ad-CCR7組(23.67±2.83)d,4組間比較差異有統(tǒng)計(jì)學(xué)意義(F=53.005,P=0.000);與空白對(duì)照組相比,imDC組、imDC+Ad組、imDC+Ad-CCR7組角膜植片存活時(shí)間均明顯延長(zhǎng)(均為P0.01);imDC+Ad-CCR7組較imDC組與imDC+Ad組均明顯延長(zhǎng)(均為P0.01)。角膜植片HE染色切片顯示,imDC+AdCCR7組的植片輕度水腫、基質(zhì)層板層纖維排列有序,未修飾imDC組和imDC+Ad組呈不同程度的水腫,基質(zhì)層板層纖維排列輕度紊亂、有少量炎性細(xì)胞;空白對(duì)照組植片高度水腫、角膜基質(zhì)板層纖維紊亂并出現(xiàn)大量炎性細(xì)胞和新生血管。結(jié)論靜脈注入CCR7基因修飾的imDC可以明顯延長(zhǎng)大鼠高危角膜移植后角膜植片存活時(shí)間,抑制角膜移植免疫排斥反應(yīng)。
[Abstract]:Aim to study the effect of recombinant adenoviruses containing chemokine receptor 7 (CCR7) gene into immature dendritic cells derived from donor bone marrow (immature dendritic cells,imDC) on immune rejection of high risk corneal transplantation in rats. Methods High risk corneal transplantation model was established by alkali burn in 60 SD rats and 30 Wistar rats. SD rats were divided into blank control group, unmodified imDC group, imDC adenoviral empty vector group (imDC Ad group and imDC CCR7 adenoviral group (imDCs Ad-CCR7 group) with 15 rats in each group. In each group, 0.1mL was injected into the tail vein 7 days before and 3 days after operation, unmodified imDC 10 脳 10 6 (0.1mL), imDC 10 脳 10 6 (0.1mL) and imDC 10 脳 10 6 (0.1mL) were injected into the tail vein 7 days before and 3 days after operation, the survival of corneal graft was observed under slit lamp and the opacification, edema and neovascularization degree of corneal graft were evaluated every day in the recipient group, and the unmodified imDC solution was injected into the tail vein 7 days before and 3 days after operation, and the unmodified imDC 10 脳 10 6 (0.1mL), imDC 10 脳 10 6 (imDC) and imDC 10 脳 10 6 (imDC) were injected into the tail vein every day after operation. On the 14th day after operation, 5 rats in each group were randomly killed and observed by HE section. Results the survival time of corneal graft was (10.44 鹵1.88) days in blank control group, (16.00 鹵2.18) days in IMDC group, (15.11 鹵2.03) days in IMDC Ad group and (23.67 鹵2.83) days in IMDC Ad-CCR7 group (F 鈮，

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