【摘要】：目的： 建立畦巴因選擇性損傷蒙古沙鼠耳蝸螺旋神經(jīng)節(jié)細胞的動物模型,在此基礎(chǔ)上研究耳蝸螺旋神經(jīng)節(jié)細胞損傷后,神經(jīng)營養(yǎng)因子-3對耳蝸螺旋神經(jīng)節(jié)細胞的保護作用,并研究探討其神經(jīng)保護作用機理。 方法： 將聽力正常的雄性蒙古沙鼠,手術(shù)暴露圓窗膜,用80umol/L哇巴因滴注于蒙古沙鼠耳蝸圓窗膜上,建立選擇性損傷螺旋神經(jīng)節(jié)細胞的動物模型。在成功建模的基礎(chǔ)上于蒙古沙鼠圓窗膜上滴注神經(jīng)營養(yǎng)因子-3(15ug/ml),穩(wěn)定7天后處死,立即取出耳蝸,制作病理切片進行HE染色,觀察Ⅰ型螺旋神經(jīng)節(jié)細胞數(shù)目、形態(tài)的改變,并用人工計數(shù)法采用SPSS17.0軟件進行統(tǒng)計學(xué)分析；制作電鏡切片,在透射電鏡下觀察細胞的超微結(jié)構(gòu),分析神經(jīng)營養(yǎng)因子-3對Ⅰ型螺旋神經(jīng)節(jié)細胞的保護作用。 結(jié)果： 本實驗用哇巴因成功建立了選擇性損傷螺旋神經(jīng)節(jié)細胞的動物模型,發(fā)現(xiàn)哇巴因?qū)Β裥吐菪窠?jīng)節(jié)細胞有明顯的損傷作用,損傷部位主要位于細胞膜周圍髓鞘組織、細胞核及線粒體,哇巴因組細胞計數(shù)較人工外淋巴液組明顯減少(P0.05)；神經(jīng)營養(yǎng)因子-3對Ⅰ型螺旋神經(jīng)節(jié)細胞的細胞膜周圍髓鞘組織、線粒體及細胞核均有保護作用,在保護組細胞計數(shù)明顯高于對照組(P0.05)。 結(jié)論： 哇巴因損傷蒙古沙鼠Ⅰ型螺旋神經(jīng)節(jié)細胞后,經(jīng)圓窗膜滴注神經(jīng)營養(yǎng)因子-3(NT-3)對Ⅰ型螺旋神經(jīng)節(jié)細胞有一定的保護作用。對于臨床上尋找保護耳蝸聽神經(jīng)細胞的有效藥物及給藥途徑具有重要的價值。
[Abstract]:Objective: to establish an animal model of selective injury of cochlear spiral ganglion cells in Mongolian gerbils, and to study the injury of cochlear spiral ganglion cells. The protective effect of neurotrophic factor-3 on cochlear spiral ganglion cells and its neuroprotective mechanism were studied. Methods: male Mongolian gerbils with normal hearing were exposed to round window membrane surgically. 80umol/L ouabain was dripped onto the cochlea round window membrane of Mongolian gerbil to establish an animal model of selective injury of spiral ganglion cells. On the basis of successful modeling, neurotrophic factor-3 (15ug/ml) was infused into the round window membrane of Mongolian gerbil. After 7 days of stability, the cochlea were taken out immediately, and pathological sections were made for HE staining to observe the number of type 鈪，

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