【摘要】：目的 1.探討鼻咽癌患者放療后齲易感性及牙本質(zhì)敏感程度的變化,分析其與放射齲之間的關(guān)系。 2.分析鼻咽癌患者放療前后唾液蛋白總量及不同分子量蛋白含量變化,定量檢測溶菌酶和乳鐵蛋白含量,探討各蛋白與放射齲之間的關(guān)系。 方法 1.刃天青試紙法檢測齲活性 2.VAS法評估牙本質(zhì)敏感程度 3.BCA法測定唾液總蛋白含量；SDS-PAGE凝膠電泳分離唾液蛋白；Quantity One專業(yè)凝膠圖像分析軟件分析掃描圖像,測得不同分子量蛋白條帶的IOD值。 4.免疫印跡法檢測唾液中溶菌酶和乳鐵蛋白含量,化學(xué)發(fā)光法獲得目標(biāo)條帶的X光膠片影像,Quantity One專業(yè)圖像分析軟件分析掃描圖像,測得目標(biāo)條帶的IOD值。 所有數(shù)據(jù)錄入Excel建立數(shù)據(jù)庫,根據(jù)原始資料多次進(jìn)行核對以確保無誤。實(shí)驗(yàn)結(jié)果導(dǎo)入SPSS16.0統(tǒng)計(jì)軟件包進(jìn)行統(tǒng)計(jì)學(xué)分析,采用重復(fù)測量資料方差分析和秩和檢驗(yàn),檢驗(yàn)水準(zhǔn)定為雙側(cè)α=0.05。 結(jié)果 1.刃天青試紙法顯示鼻咽癌患者放療后齲活性增強(qiáng)(P0.05)。 2.鼻炎癌患者放療后牙本質(zhì)敏感度增加(P0.05)。 3.BCA法測定鼻咽癌病人非刺激性全唾液中總蛋白含量(μg/ml)結(jié)果顯示：放療后蛋白總量低于放療前(P0.05,放療前為875.04±114.93,放療后為773.71±116.46)。SDS-PAGE凝膠電泳圖片顯示分子量約14.5 kD、28kD、38 kD、56 kD、80 kD位置的5種蛋白條帶較穩(wěn)定出現(xiàn)。其中分子量為14.5 kD、28kD、38 kD、56 kD的四種蛋白豐度值和百分含量放療后均低于放療前(P0.05)。80 kD蛋白豐度值和百分含量放療后高于放療前(P0.05)。 4.免疫印跡法檢測鼻咽癌患者放療前后唾液中溶菌酶和乳鐵蛋白含量,結(jié)果顯示：溶菌酶豐度值(IOD)和百分含量放療后低于放療前(P0.05,放療前后豐度值分別為64.46±37.97、37.39±28.83;百分含量為6.80±2.20、4.58±1.59)；放療前后乳鐵蛋白豐度值和百分含量無明顯統(tǒng)計(jì)學(xué)差異(P0.05,放療前后豐度值分別是46.26±30.32、46.28±31.46；百分含量是2.50±1.62、2.82±1.62)。重復(fù)測量資料方差分析結(jié)果顯示兩種蛋白之間存在相互作用(P0.05)。 結(jié)論 1.放療后鼻咽癌患者的齲活性增強(qiáng),同時(shí)牙本質(zhì)的敏感程度增加。 2.鼻咽癌患者唾液中分子量為14.5kD、28 kD、38 kD、56 kD四種蛋白的豐度值和百分含量放療后低于放療前,80 kD的蛋白豐度值和百分含量放療后高于放療前。 3.溶菌酶豐度值和百分含量放療后低于放療前,乳鐵蛋白豐度值和百分含量在放療前后無明顯改變。
[Abstract]:Objective 1. To investigate the changes of susceptibility to caries and dentin sensitivity in patients with nasopharyngeal carcinoma (NPC) after radiotherapy, and to analyze the relationship between them and radiation caries. 2. The changes of total salivary protein and different molecular weight proteins before and after radiotherapy in patients with nasopharyngeal carcinoma were analyzed. Lysozyme and lactoferrin contents were detected quantitatively and the relationship between each protein and radiation caries was discussed. Method 1. The sensitivity of dentin was evaluated by 2.VAS method, total protein content in saliva was determined by 3.BCA method, saliva protein was separated by SDS-PAGE gel electrophoresis. The scanning images were analyzed by Quantity One gel image analysis software, and the IOD values of different molecular weight protein bands were measured. 4. The contents of lysozyme and lactoferrin in saliva were detected by Western blotting. The scanning images were analyzed by, Quantity One software and the IOD values of target bands were measured by chemiluminescence. All data entered into the Excel to establish a database, according to the original data repeated checks to ensure that the correct. The experimental results were imported into SPSS16.0 statistical software package for statistical analysis, and repeated measurement data were used for variance analysis and rank sum test. The test level was set as bilateral 偽 = 0.05. Result 1. Edge azure test paper method showed that the caries activity of patients with nasopharyngeal carcinoma increased after radiotherapy (P0.05). 2. The sensitivity of dentin increased after radiotherapy in patients with rhinitis cancer (P0.05). The total protein content (渭 g/ml) in non-irritated whole saliva of nasopharyngeal carcinoma patients was determined by 3.BCA method. The results showed that the total protein content after radiotherapy was lower than that before radiotherapy (P 0.05, 875.04 鹵114.93 before radiotherapy). After radiotherapy, 773.71 鹵116.46). SDS-PAGE gel electrophoresis images showed that five protein bands with molecular weight of about 14. 5 kD,28kD,38 kD,56 kD,80 kD appeared stably. The protein abundance and percentage content of the four proteins with molecular weight of 14. 5 kD,28kD,38 kD,56 kD were lower after radiotherapy than before radiotherapy (P0.05). 80 kD protein abundance and percentage content after radiotherapy were higher than those before radiotherapy (P0.05). 4. The levels of lysozyme and lactoferrin in saliva of patients with nasopharyngeal carcinoma before and after radiotherapy were detected by Western blotting. The results showed that the abundance of lysozyme (IOD) and percentage after radiotherapy were lower than those before radiotherapy (P 0.05). The abundance before and after radiotherapy was 64.46 鹵37.97 鹵37.39 鹵28.83; The percentage content was 6.80 鹵2.20 鹵4.58 鹵1.59). There was no significant difference in the abundance and percentage of lactoferrin before and after radiotherapy (P 0.05, 46.26 鹵30.32 鹵46.28 鹵31.46 and 2.50 鹵1.622 鹵2.82 鹵1.62, respectively). The results of ANOVA showed that there was interaction between the two proteins (P0.05). Conclusion 1. After radiotherapy, the caries activity of nasopharyngeal carcinoma patients increased and the sensitivity of dentin increased. 2. The abundance and percentage of four proteins in saliva of nasopharyngeal carcinoma patients with molecular weight of 14.5 kDX 28 kD,38 kD,56 kD were lower after radiotherapy than those before radiotherapy, and the protein abundance and percentage content of 80 kD after radiotherapy were higher than those before radiotherapy. 3. The abundance and percentage of lysozyme after radiotherapy were lower than those before radiotherapy, but lactoferrin abundance and percentage did not change significantly before and after radiotherapy.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R739.63

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