【摘要】：目的檢測谷氨酰胺誘導(dǎo)HSP70在正常視網(wǎng)膜內(nèi)的表達(dá)及隨時(shí)間的變化。 方法健康清潔型Wistar大鼠90只隨機(jī)分為三組,正常對照組10只、陽性對照組即硫酸鋅腹腔注射組10只、實(shí)驗(yàn)組即谷氨酰胺腹腔注射組70只。實(shí)驗(yàn)組分別于谷氨酰胺注射(0.75 g/Kg)后1h,4h,6h,12h,24h,48h,72h各隨機(jī)處死10只大鼠。陽性對照組于硫酸鋅注射(24.6mg/Kg)后12h處死大鼠。正常對照組,陽性對照組及實(shí)驗(yàn)組各時(shí)間點(diǎn)10只老鼠中隨機(jī)取5只用于免疫組織化學(xué)染色,剩余5只用于Western Blot分析。 結(jié)果Western Blot分析顯示,正常對照組僅有HSP70的微量表達(dá)。谷氨酰胺注射1h后HSP70的表達(dá)稍有增強(qiáng),4h后達(dá)到高峰并持續(xù)到12h,24h與48h組表達(dá)較前減少,但這兩組之間無顯著差異。72h組顯著減少,但仍高于正常對照組。免疫組織化學(xué)染色顯示誘導(dǎo)表達(dá)的HSP70在視網(wǎng)膜各層細(xì)胞均有明顯表達(dá),而正常對照組HSP70僅微量表達(dá)于視網(wǎng)膜內(nèi)節(jié)層、外界膜及外核層。免疫組化染色陽性細(xì)胞光密度值測定結(jié)果與Western Blot結(jié)果一致。 結(jié)論1.正常情況下,視網(wǎng)膜微量表達(dá)HSP70 2.腹腔注射谷氨酰胺可以誘導(dǎo)大鼠視網(wǎng)膜HSP70的明顯表達(dá)。
[Abstract]:Objective to detect the expression and changes of glutamine induced HSP70 in normal retina. Methods 90 healthy and clean Wistar rats were randomly divided into three groups: normal control group (n = 10), positive control group (n = 10) and glutamine intraperitoneal injection group (n = 70). In the experimental group, 10 rats were randomly killed at 1 h, 4 h, 6 h, 12 h, 24 h, 48 h and 72 h after injection of glutamine (0. 75 g/Kg). The rats in the positive control group were killed 12 hours after zinc sulfate injection (24.6mg/Kg). Five of the 10 mice in the control group, the positive control group and the experimental group were randomly selected for immunohistochemical staining, and the remaining 5 rats were used for Western Blot analysis. Results Western Blot analysis showed that there was only a trace expression of HSP70 in normal control group. The expression of HSP70 increased slightly after 1 h of glutamine injection, reached a peak at 4 h, and continued to decrease at 12 h and 48 h, but there was no significant difference between the two groups. The expression of HSP70 in 72 h group was significantly decreased, but still higher than that in normal control group. Immunohistochemical staining showed that the expression of HSP70 was significantly expressed in all layers of the retina, while in the normal control group, it was only expressed in the inner ganglion layer, outer membrane and outer nuclear layer of the retina. The results of optical density of positive cells by immunohistochemical staining were consistent with those of Western Blot. Conclusion 1. Under normal conditions, HSP70 2. 2 was expressed in retina. Intraperitoneal injection of glutamine could induce the expression of HSP70 in rat retina.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R77

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前2條

1 紀(jì)珍;谷氨酰胺對大鼠視網(wǎng)膜興奮性損傷的保護(hù)作用[D];青島大學(xué);2013年

2 邢業(yè)嬌;替普瑞酮對大鼠視網(wǎng)膜興奮性損傷的保護(hù)作用[D];青島大學(xué);2013年

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本文編號(hào)：2387525