本文選題：喉腫瘤 + 癌�。� 參考：《青島大學》2013年碩士論文

【摘要】：目的通過檢測喉鱗狀細胞癌(鱗癌)腫瘤細胞中人乳頭狀瘤病毒(Human papillo-mavirus,HPV)的DNA及其亞型的表達,探討HPV感染與喉鱗癌的關(guān)系及在喉鱗癌發(fā)病中的意義。 方法收集2011年12月-2013年03月間,青島大學醫(yī)學院附屬醫(yī)院耳鼻咽喉頭頸外科初治喉癌患者82例,男性80例,女性2例,37-83歲,平均(60.3±9.6)歲,均經(jīng)病理檢查診斷為喉鱗狀細胞癌,無喉乳頭狀瘤惡變者。對照組為同期喉乳頭狀瘤患者8例,均為男性,51-61歲,平均(57.8±3.2)歲,術(shù)后均經(jīng)病理檢查證實為喉乳頭狀瘤。將術(shù)中切除的新鮮病變組織以棉拭子刷取腫瘤組織細胞,并存放至細胞保存液中。采用聚合酶鏈式反應(yīng)(Polymerase Chain Reaction,PCR)擴增和DNA反向點雜交相結(jié)合的DNA芯片技術(shù),檢測兩組細胞HPV DNA及其亞型的表達。 結(jié)果喉癌82例HPV DNA檢出者5例,喉乳頭狀瘤組8例檢出者6例。喉癌組HPVDNA撿出率為6.10%(5/82)較喉乳頭狀瘤組75.00%(6/8)低,差異具有極顯著性意義(X2=26.15,P=0,P＜0.01)。喉癌組高危型HPV16DNA檢出者3例,占3.66%,低危型HPV11DNA與HPV43DNA各1例,分別占1.22%。喉乳頭狀瘤組HPV16DNA撿出者2例,占25.00%.HPV11、HPV6DNA各2例,分別占25.00%。喉癌組高危型HPV16DNA與低危型HPV43DNA檢出率分別與喉乳頭狀瘤組比較,差異均無顯著性意義(P=0.061、1.0,P＞0.05)。喉乳頭狀瘤組低危型HPV11DNA與HPV6DNA檢出率較喉癌組高,差異均有顯著性意義(P=0.02、0.007,P0.05)。 結(jié)論本研究觀察到HPV感染與喉鱗癌有關(guān),HPV DNA檢出率為6.1%;HPV DNA陽性的喉鱗癌患者中以高危型HPV16為主,占60%。 目的通過檢測喉咽鱗狀細胞癌(喉咽癌)中人乳頭狀瘤病毒(HPV)的DNA及其亞型的表達,探討HPV感染與喉咽癌的關(guān)系及在喉咽癌發(fā)病中的意義。 方法收集2011年12月-2013年03月間,青島大學醫(yī)學院附屬醫(yī)院耳鼻咽喉頭頸外科初治喉咽癌患者36例,男性35例,女性1例,42-77歲,平均(57.4±8.2)歲,均經(jīng)病理檢查診斷為喉咽鱗狀細胞癌,術(shù)中以棉拭子刷取腫瘤細胞。對照組患者35例,男性32例,女性3例,42-75歲,平均(55.1±8.1)歲,其中聲帶息肉患者26例,會厭囊腫患者9例,術(shù)中以棉拭子刷取喉咽部黏膜脫落細胞。采用聚合酶鏈式反應(yīng)(PCR)擴增和DNA反向點雜交相結(jié)合的DNA芯片技術(shù),檢測兩組細胞HPV DNA及其亞型的表達。 結(jié)果喉咽癌組36例中HPV DNA檢出者6例,HPV DNA檢出率16.7%(6/36)與對照組(0%)比較,差異有顯著性意義(x2=4.40,P=0.036,P0.05).喉咽癌組中高危型HPV DNA檢出者5例,占83.3%,其中HPV16DNA2例,HPV18、59、66DNA各1例；低危型HPV DNA檢出者僅有1例,為HPV43。喉咽癌組高危型HPV DNA檢出者與對照組比較,差異無顯著性意義(x2=3.323,P=0.068,P0.05).喉咽癌組低危型HPV DNA檢出者與對照組比較,差異亦無顯著性意義(P=1.00,P0.05). 結(jié)論本研究觀察到HPV感染與喉咽鱗癌有關(guān),HPV DNA撿出率為16.7%；HPVDNA陽性的喉咽鱗癌患者中以高危型HPV為主,占83.3%。
[Abstract]:Objective to investigate the relationship between HPV infection and laryngeal squamous cell carcinoma (LSCC) and its significance in the pathogenesis of laryngeal squamous cell carcinoma (LSCC) by detecting the expression of human papillo-mavirusus (HPVV) DNA and its subtypes in human laryngeal squamous cell carcinoma (LSCC). Methods from December 2011 to March 2013, 82 patients with primary laryngeal carcinoma in the Department of Otorhinolaryngology and head and neck surgery, affiliated Hospital of Qingdao University, were collected, including 80 males and 2 females aged 37-83 years, with an average age of 60.3 鹵9.6 years. All patients were diagnosed as laryngeal squamous cell carcinoma by pathological examination. There was no malignant change in laryngeal papilloma. There were 8 cases of laryngeal papilloma in the control group, all of them were male (51-61 years old, mean 57.8 鹵3.2) years old. All cases were proved to be laryngeal papilloma by pathological examination. The tumor cells were removed by cotton swab and stored in the cell preservation solution. DNA microarray was used to detect the expression of HPV DNA and its subtypes in two groups of cells by polymerase chain reaction (PCR) amplification and DNA reverse dot hybridization. Results HPV DNA was detected in 5 cases in 82 cases of laryngeal carcinoma and 6 cases in 8 cases of laryngeal papilloma. The DNA extraction rate of HPV in laryngeal carcinoma group was 6.10 / 82, which was significantly lower than that in laryngeal papilloma group (75.00 / 6 / 8), and the difference was significant (P < 0.01). High risk HPV 16 DNA was detected in 3 cases (3.66%) in laryngeal carcinoma group, and 1 case (1.22%) in low risk type HPV11 DNA and 1 case HPV43 DNA respectively. In the laryngeal papilloma group, 2 cases of HPV16 DNA were picked out, 2 cases of HPV16 DNA were found in 25.00 cases, 2 cases of HPV11 HPV6 DNA were found in the laryngeal papilloma group, accounting for 25.00% of the DNA, respectively. The detection rate of high risk HPV16 DNA and low risk HPV 43 DNA in laryngeal carcinoma group was not significantly different from that in laryngeal papilloma group (P > 0.05). The detection rate of HPV11 DNA and HPV6 DNA in laryngeal papilloma group was higher than that in laryngeal carcinoma group (P < 0.05). Conclusion in this study, we observed that the detection rate of HPV DNA in laryngeal squamous cell carcinoma associated with HPV infection was 6.1 and HPV16 was the most common type in laryngeal squamous cell carcinoma, accounting for 60 cases. Objective to investigate the relationship between human papillomavirus (HPV) infection and laryngeal carcinoma by detecting the expression of HPVDNA and its subtypes in laryngeal squamous cell carcinoma (LSCC) and its significance in the pathogenesis of laryngopharyngeal carcinoma. Methods from December 2011 to March 2013, 36 patients with laryngopharynx carcinoma treated in the Department of Otorhinolaryngology and neck surgery, affiliated Hospital of Qingdao University Medical College, were collected, including 35 males and 1 female, aged 42-77 years, with an average of 57.4 鹵8.2 years old. All patients were diagnosed as laryngopharyngeal squamous cell carcinoma by pathological examination. The tumor cells were removed with cotton swab during operation. 35 patients in the control group, 32 males and 3 females aged 42-75 years, with an average age of 55.1 鹵8.1 years, including 26 patients with vocal cord polyps and 9 patients with epiglottic cyst. DNA microarray was used to detect the expression of HPV DNA and its subtypes in two groups of cells by polymerase chain reaction (PCR) amplification and reverse dot hybridization. Results the detection rate of HPV DNA in 6 out of 36 patients with laryngopharyngeal carcinoma was 16. 7 / 36), which was significantly higher than that in the control group (P 0. 05). High risk HPV DNA was detected in 5 cases (83.3%) in laryngopharyngeal carcinoma group, including 2 cases of HPV16 DNA, 1 case of HPV1859.66 DNA, and 1 case of low risk type HPV DNA (HPV43). There was no significant difference in high risk HPV DNA detection between the larynx carcinoma group and the control group. There was no significant difference between the low risk HPV DNA detection group and the control group. Conclusion in this study, the detection rate of HPV DNA in larynx squamous cell carcinoma associated with HPV infection was 16.7% (83.3%) in patients with HPV DNA positive larynx pharynx squamous cell carcinoma.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R739.6

【參考文獻】

相關(guān)期刊論文 前10條

1 管國芳，許亞輝，孫立群;喉癌與人類乳頭狀瘤病毒相關(guān)性的研究[J];白求恩醫(yī)科大學學報;1994年02期

2 葉青;趙舒薇;何金;強筆;英信江;;喉癌組織中HPV16及其早期區(qū)基因E6、E7蛋白的表達及意義[J];第二軍醫(yī)大學學報;2007年01期

3 楊丹球;陳鳳坤;唐惠英;李松遠;黃秋霞;施國元;李瑩;何遠超;吳鏗;;廣西沿海地區(qū)婦女宮頸癌患者人乳頭瘤病毒(HPV)感染各亞型分布特點[J];中國婦產(chǎn)科臨床雜志;2011年01期

4 周健,陳全錄,沈瓊,谷淑燕,曾毅;食管癌標本中人乳頭狀瘤病毒(HPV)DNA序列的檢測[J];河南醫(yī)科大學學報;1987年01期

5 王鑫,韓瑞珠,李曉丹,徐秀玉;HPV16、18E6基因在喉癌組織中的表達[J];哈爾濱醫(yī)科大學學報;2002年04期

6 吳俊福;周佳青;;HPV與頭頸部腫瘤的關(guān)系[J];現(xiàn)代腫瘤醫(yī)學;2011年10期

7 盧國偉;王琳;孫彥;李薇;華輝;葛瑞峰;;山東東部57例喉癌人乳頭狀瘤病毒DNA及其亞型的檢測[J];山東大學耳鼻喉眼學報;2012年06期

8 張華,鄭艷,蘇時務(wù),陳朝倫,盧曉梅,沈?qū)氁?新疆不同民族喉乳頭狀瘤與喉癌組織中不同類型HPV的檢測[J];新疆醫(yī)科大學學報;2002年04期

9 李良忠,蔣萍麗;維吾爾族口腔鱗癌患者HPV感染及PCNA表達的臨床病理學意義[J];新疆醫(yī)科大學學報;2005年10期

10 陸牡丹;許飛;陳道楨;;人乳頭瘤病毒感染現(xiàn)狀的分析[J];中國婦幼保健;2011年17期

相關(guān)博士學位論文 前1條

1 黃輝;口咽部鱗狀細胞癌人乳頭狀瘤病毒感染及p16蛋白表達的臨床意義[D];北京協(xié)和醫(yī)學院;2012年

相關(guān)碩士學位論文 前2條

1 黃岸瑞;HPV16/18在下咽鱗癌及其頸淋巴結(jié)的表達研究[D];廣西醫(yī)科大學;2011年

2 張潔莉;HPV感染與頭頸部鱗癌的相關(guān)性以及對腫瘤預(yù)后的影響[D];北京協(xié)和醫(yī)學院;2010年

，

本文編號：2034229