本文選題：脈絡(luò)膜腫瘤 + 黑色素瘤��； 參考：《青島大學(xué)》2011年碩士論文

【摘要】：目的探討脈絡(luò)膜黑色素瘤中侵襲與轉(zhuǎn)移相關(guān)因子縫隙連接蛋白43(Cx43)、上皮型鈣黏附分子(E-cadherin)、磷脂酰肌醇-3激酶(PI3K)和結(jié)締組織生長(zhǎng)因子(CTGF)的表達(dá)及意義。 方法實(shí)驗(yàn)研究。采用免疫組織化學(xué)法檢測(cè)27例脈絡(luò)膜黑色素瘤組織及15例色素痣組織中Cx43、E-cadherin、P13K和CTGF的表達(dá),并與臨床病理資料做對(duì)照分析,采用x2檢驗(yàn)比較各因子表達(dá)陽(yáng)性率的差異。 結(jié)果Cx43、E-cadherin、P13K及CTGF蛋白在脈絡(luò)膜黑色素瘤中的表達(dá)率分別為74.07%(20/27),44.4%(12/27),74.07%(20/27)和66.67%(18/27),在色素痣中的表達(dá)率分別為33.33%(5/15),86.67%(13/15),33.33%(5/15)和20.00%(3/15),Cx43、P13K及CTGF蛋白在脈絡(luò)膜黑色素瘤中的表達(dá)較色素痣增加,差異有統(tǒng)計(jì)學(xué)意義(x2=5.060,P=0.024；x2=5.060,P=0.024；x2=6.637,P=0.010)。E-cadherin蛋白在脈絡(luò)膜黑色素瘤中的表達(dá)較色素痣降低,差異有統(tǒng)計(jì)學(xué)意義(x2=5.490,P=0.019)。Cx43蛋白在不同組織學(xué)類(lèi)型的脈絡(luò)膜黑色素瘤中陽(yáng)性表達(dá)率不同：梭形細(xì)胞型為40%(4/10),混合型為88.89%(8/9),類(lèi)上皮型為100%(8/8),差異有統(tǒng)計(jì)學(xué)意義(x2=9.874,P=0.007)。P13K蛋白在不同大小的脈絡(luò)膜黑色素瘤中陽(yáng)性表達(dá)率不同：小腫瘤為42.86%(3/7),中腫瘤為75%(9/12),大腫瘤為100%(8/8),差異有統(tǒng)計(jì)學(xué)意義(x2=6.357,P=0.042)。Cx43、E-cadherin、P13K及CTGF蛋白在未累及鞏膜的脈絡(luò)膜黑色素瘤中的表達(dá)率分別為50%(6/12),83.33%(10/12),50%(6/12)和41.66%(5/12),在累及鞏膜的脈絡(luò)膜黑色素瘤中的表達(dá)率分別為93.33%(14/15),40%(6/15),93.33%(14/15)和86.67%(13/15),Cx43、PI3K及CTGF蛋白在脈絡(luò)膜黑色素瘤中的表達(dá),累及鞏膜組較未累及組增加,差異有統(tǒng)計(jì)學(xué)意義(x2=4.457,P=0.016；x2=4.457,P=0.016；x2=4.218,P=0.019)。E-cadherin在脈絡(luò)膜黑色素瘤中的表達(dá),累及鞏膜組較未累及組降低,差異有統(tǒng)計(jì)學(xué)意義(x2=3.546,P=0.028)。 結(jié)論Cx43、P13K、CTGF蛋白的高表達(dá)及E-cadherin蛋白的低表達(dá)與脈絡(luò)膜黑色素瘤的浸潤(rùn)和轉(zhuǎn)移可能有密切的關(guān)系。
[Abstract]:Objective to investigate the expression and significance of invasion and metastasis related factor gap junction protein 43Cx43C, E-cadherinus, phosphatidylinositol 3 kinase (PI3K) and connective tissue growth factor (CTGF) in choroidal melanoma. Methods Experimental study was carried out. Immunohistochemical method was used to detect the expression of P13K and CTGF in 27 cases of choroidal melanoma and 15 cases of nevus pigmentosa. Results the expression rates of P13K and CTGF protein in choroidal melanoma were 74.07 and 74.44, respectively. The expression rates of P13K and CTGF protein in choroidal melanoma were 33. 33 / 515 / 86.67 and 33. 33% and 20 / 27, respectively. The expression rates of Cx43P13K and CTGF protein in choroidal melanoma were significantly higher than those in nevus of choroidal melanoma, and the expression of Cx433P13K and CTGF protein in choroidal melanoma was higher than that in nevus of choroidal melanoma, respectively. The expression rate of Cx43P13K and CTGF protein in choroidal melanoma was higher than that in nevus, and the expression rate of Cx43P13K and CTGF protein in choroidal melanoma was higher than that in nevus. The expression of E-cadherin protein in choroidal melanoma was significantly lower than that in nevus pigmentosa, and the expression of E-cadherin protein in choroidal melanoma was significantly lower than that in nevus pigmentosa. The difference was statistically significant in the positive rate of the protein in different histological types of choroidal melanoma: the spindle cell type was 40 / 10, the mixed type was 88.89 / 8 / 9, and the epithelioid type was 100 / 8 / 8 / 8. The difference was statistically significant (x29.874P0.007N. P13K protein) in different sizes of choroidal melanoma. The positive expression rates of Cx43E-cadherin P13K and CTGF protein in choroidal melanoma were 42.86%, 75 / 9 / 12 and 100 / 8 / 8, respectively. The difference was statistically significant. The expression rates of Cx43E-cadherin P13K and CTGF protein in non-scleral choroidal melanoma were 50 / 612 / 83.33 / 10 / 10 / 50 / 612) and 41.66% / 512P / r. The expression rates of Cx43 PI3K and CTGF protein in choroidal melanoma involving sclera were 93.3314 / 15, 93.3314 / 15, 93.3314 / 15 and 86.67%, respectively, and the expression of Cx43 PI3K and CTGF protein in choroidal melanoma. The expression of E-cadherin in choroidal melanoma was significantly higher in scleral group than that in non-involvement group, and the difference was statistically significant (P 0.016). The expression of E-cadherin in choroidal melanoma was significantly lower in scleral group than in non-involvement group, and the difference was statistically significant (P 0.02828) between the scleral group and the scleral group (P 0.016, P 0.016, P = 4.457, P = 0.021, P < 0.05), and the expression of E-cadherin in the scleral group was significantly lower than that in the sclera group, which was significantly lower than that in the sclera group. Conclusion the high expression of CTGF and the low expression of E-cadherin may be closely related to the invasion and metastasis of choroidal melanoma.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R739.7

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