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  本文選題：上皮間質(zhì)轉(zhuǎn)化 + 聲門上型喉��； 參考：《中南大學(xué)》2013年碩士論文

【摘要】：目的：檢測聲門上型喉癌組織及聲帶息肉組織標(biāo)本中的Twist、 E-cadherin和N-cadherin蛋白表達,探討以上三種蛋白在喉部良惡性組織中的差異性表達及其與聲門上型喉癌患者臨床病理特征的關(guān)系,以及三種蛋白表達相關(guān)性。結(jié)合臨床隨訪資料,分析上述三種蛋白表達與聲門上型喉癌預(yù)后之間的關(guān)系。 方法：采用免疫組化方法檢測46例聲門上型喉癌組織及30例聲帶息肉組織中的Twist、E-cadherin及N-cadherin蛋白的表達。 結(jié)果： 1.Twist、E-cadherin及N-cadherin蛋白在聲門上型喉癌的陽性表達率分別是69.57%、39.13%、50.00%,在聲帶息肉組織中分別為36.67%、86.67%、13.33%,以上三種蛋白在喉部良惡性組織中的差異有統(tǒng)計學(xué)意義(p0.05)。 2.低分化、淋巴結(jié)陽性、T3-4、中晚期的聲門上型喉癌組織中的Twist蛋白和N-cadherin蛋白的陽性表達率高于在中高分化、淋巴結(jié)陰性、T1-2、早期的聲門上型喉癌組織中的陽性表達率(p0.05)。低分化、淋巴結(jié)陽性、T3-4、中晚期的聲門上型喉癌中的E-cadherin蛋白陽性表達率低于在中高分化、淋巴結(jié)陰性、T1-2、早期的聲門上型喉癌組織的陽性表達率(p0.05)。 3.相關(guān)分析示：在聲門上型喉癌中Twist蛋白表達與E-cadherin蛋表達負相關(guān)(r=-0.535,p=0.000)；而Twist蛋白表達則與N-cadherin蛋白表達正相關(guān)(r=0.378,p=0.010)；E-cadherin蛋白表達與N-cadherin蛋白表達呈負相關(guān)(r=-0.445,p=0.002)。 4. Twist蛋白及N-Cadherin蛋白陽性表達組的3年局部復(fù)發(fā)、頸淋巴結(jié)復(fù)發(fā)高于陰性組(p0.05),E-Cadherin蛋白陽性表達組的3年局部復(fù)發(fā)、頸淋巴結(jié)復(fù)發(fā)低于陰性組(p0.05)。 5. Twist蛋白、N-cadherin表達陽性的3年總OS及DFS均顯著低于陰性組(p0.05)。E-cadherin表達陽性的3年OS及DFS均顯著高于陰性組(p0.05)。 6.Cox回歸模型多因素分析示：腫瘤浸潤深度及淋巴結(jié)轉(zhuǎn)移情況是影響聲門上型喉癌患者3年OS的獨立預(yù)后因素(HR分別為8.639、4.276,p值分別為0.037、0.023)。 結(jié)論： 1.Twist、 E-cadherin及N-cadherin蛋白與聲門上型喉癌的發(fā)生有關(guān)。 2.Twist、E-cadherin及N-cadherin與聲門上型喉癌的侵襲、轉(zhuǎn)移等有關(guān)。 3.Twist蛋白可能與聲門上型喉癌中上皮間質(zhì)轉(zhuǎn)化的發(fā)生有關(guān)。 4.Twist、E-cadherin與N-cadherin蛋白可能與聲門上型喉癌預(yù)后有關(guān)。
[Abstract]:Objective: to detect the expression of Twist, E-cadherin and N-cadherin proteins in supraglottic laryngeal carcinoma and vocal cord polyp tissues, and to explore the relationship between the expression of these three proteins in benign and malignant laryngeal tissues and the clinicopathological features of supraglottic laryngeal carcinoma. And three kinds of protein expression correlation. To analyze the relationship between the expression of these three proteins and the prognosis of supraglottic laryngeal carcinoma. Methods: the expression of Twister-E-cadherin and N-cadherin protein in 46 cases of supraglottic laryngeal carcinoma and 30 cases of vocal cord polyp were detected by immunohistochemical method. Results: 1. The positive expression rates of Twister-E-cadherin and N-cadherin protein in supraglottic laryngeal carcinoma were 69.57and 39.1350.The positive rates of Twister-E-cadherin and N-cadherin protein were 39.57and 39.130.The positive rates of Twister-E-cadherin and N-cadherin protein in vocal cord polyps were 36.670.67 and 13.33g, respectively. There was a significant difference among the above three proteins in benign and malignant larynx tissues (p 0.05). 2. The positive expression rate of Twist protein and N-cadherin protein in advanced supraglottic laryngeal carcinoma was higher than that in middle and high differentiation, lymph node negative T1-2, and early supraglottic laryngeal carcinoma (p0.05). The positive expression rate of E-cadherin protein in advanced glottic laryngeal carcinoma was lower than that in middle and high differentiation, lymph node negative tumor T1-2, and the positive expression rate of p0.05in early supraglottic laryngeal carcinoma. 3. Correlation analysis showed that the expression of Twist protein was negatively correlated with the expression of E-cadherin eggs in supraglottic laryngeal carcinoma, while the expression of Twist protein was positively correlated with the expression of N-cadherin protein. There was a negative correlation between the expression of E-cadherin protein and the expression of N-cadherin protein in supraglottic laryngeal carcinoma. 4. The local recurrence of Twist protein and N-Cadherin protein positive group was 3 years, and the recurrence of cervical lymph node was higher than that of negative group. The recurrence of cervical lymph node was lower than that of negative group. 5. The expression of Twist protein N-cadherin was significantly lower in the 3-year total OS and DFS than that in the negative group. The expression of DFS and OS in the negative group was significantly higher than that in the negative group. The multivariate analysis of 6.Cox regression model showed that the depth of tumor invasion and lymph node metastasis were independent prognostic factors for the patients with supraglottic laryngeal carcinoma for 3 years. The HR was 8.639 鹵4.276 (P = 0.037) and 0.023 ~ (th), respectively. Conclusion: 1. Twist. E-cadherin and N-cadherin proteins were associated with supraglottic laryngeal carcinoma. 2. Twistoma E-cadherin and N-cadherin were associated with the invasion and metastasis of supraglottic laryngeal carcinoma. The 3.Twist protein may be associated with epithelial interstitial transformation in supraglottic laryngeal carcinoma. 4. Twistus E-cadherin and N-cadherin protein may be related to the prognosis of supraglottic laryngeal carcinoma.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R739.65

【參考文獻】

相關(guān)期刊論文 前1條

1 夏良平,曾宗淵,魏茂文,許光普,郭朱明,張詮,陳文寬;影響喉癌生存率的單因素和多因素分析[J];中國腫瘤;2002年11期

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本文編號：1910172