本文選題：鼻炎 + 變應(yīng)性��； 參考：《蘇州大學(xué)》2013年碩士論文

【摘要】：目的：探討鼻腔滴注重組小鼠白細(xì)胞介素 22（recombination murine interleukin 22/rm IL 22）對(duì)小鼠變應(yīng)性鼻炎免疫應(yīng)答的調(diào)節(jié)作用。 方法：將24只6~8周的雌性BALB/c小鼠隨機(jī)分為3組，每組8只，分別為對(duì)照組、模型組和IL 22組。模型組和IL 22組建立小鼠變應(yīng)性鼻炎模型，同時(shí)IL 22組在每次激發(fā)前鼻腔滴注rm IL 22。首先應(yīng)用間接免疫熒光法檢測(cè)鼻黏膜上皮細(xì)胞是否表達(dá)IL 22受體（IL 22R1），最后一次激發(fā)后記錄三組小鼠10分鐘的抓撓數(shù)和噴嚏數(shù)，應(yīng)用酶聯(lián)免疫吸附法（enzyme linked immunosorbent assay, ELISA）檢測(cè)外周血清中卵白蛋白 特異性IgE（OVA sIgE）的濃度，，檢測(cè)鼻腔灌洗液中干擾素 γ（IFN γ）、白細(xì)胞介素 4（IL 4）、白細(xì)胞介素 5（IL 5）、白細(xì)胞介素 10（IL 10）和胸腺基質(zhì)淋巴細(xì)胞生成素（thymic stromal lymphopoietin, TSLP）的濃度，HE染色觀察鼻黏膜局部嗜酸性粒細(xì)胞浸潤(rùn)情況。最后利用SPSS17.0統(tǒng)計(jì)學(xué)軟件對(duì)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析，以P0.05為差異有統(tǒng)計(jì)學(xué)意義。 結(jié)果：IL 22R1在小鼠鼻黏膜上皮細(xì)胞表達(dá)，IL 22組較模型組小鼠變應(yīng)性鼻炎抓撓和噴嚏癥狀減輕，鼻腔灌洗液中IL 4、IL 5和外周血清中OVA sIgE的濃度較模型組降低，局部嗜酸性粒細(xì)胞數(shù)侵潤(rùn)減少，三組間差異有統(tǒng)計(jì)學(xué)意義，鼻腔灌洗液中IFN γ、IL 10和TSLP的濃度在IL 22組和模型組間差異無(wú)統(tǒng)計(jì)學(xué)意義。 結(jié)論：鼻腔滴注rm IL 22可以抑制變應(yīng)性鼻炎小鼠的免疫應(yīng)答，有望成為治療變應(yīng)性鼻炎的一種新方法。 目的：探討重組1、2和8型腺相關(guān)病毒（rAAV1、rAAV2和rAAV8）載體經(jīng)小鼠鼻腔滴注轉(zhuǎn)導(dǎo)增強(qiáng)綠色熒光蛋白（EGFP）基因在鼻黏膜上皮細(xì)胞的表達(dá)情況。 方法：6只6~8周的雌性BALB/c小鼠隨機(jī)分為3組，每組2只。用帶有EGFP基因的重組1、2和8型腺相關(guān)病毒進(jìn)行小鼠鼻腔滴注，第4周處死小鼠，獲得小鼠完整鼻腔，固定脫鈣后冰凍切片，然后在熒光顯微鏡下觀察EGFP在三組小鼠鼻黏膜上皮細(xì)胞的表達(dá)情況。 結(jié)果：rAAV1載體攜帶的EGFP成功轉(zhuǎn)染，第4周穩(wěn)定表達(dá)，另外兩型重組腺相關(guān)病毒攜帶的EGFP均未見在小鼠鼻黏膜表達(dá)。 結(jié)論：rAAV1載體可以轉(zhuǎn)染小鼠鼻腔黏膜上皮細(xì)胞，并穩(wěn)定表達(dá)攜帶的EGFP，可以作為鼻部疾病基因治療的載體。
[Abstract]:Aim: to investigate the regulatory effect of 22(recombination murine interleukin 22/rm IL-22 on the immune response in mice with allergic rhinitis.Methods: Twenty-four female BALB/c mice aged 6 to 8 weeks were randomly divided into 3 groups: control group, model group and IL-22 group.Model group and IL-22 group were used to establish allergic rhinitis model in mice.The expression of IL-22 receptor IL-22R1 was detected by indirect immunofluorescence assay. After the last stimulation, the number of scratches and sneezes in the three groups were recorded for 10 minutes.The concentration of ovalbumin specific IgE(OVA in peripheral serum was detected by enzyme enzyme linked immunosorbent assay (Elica) by enzyme linked immunosorbent assay (Elisa).Detection of IFN- 緯, 4(IL, 5(IL and 10(IL in nasal lavage fluid and thymic stromal lymphopoietin (TSLP) in nasal lavage fluidGranulocyte infiltration.Finally, SPSS17.0 statistical software was used for statistical analysis of the data, with P0.05 as the difference was statistically significant.Results compared with the model group, the symptoms of scratching and sneezing of mice with allergic rhinitis were alleviated, and the concentrations of IL-4 and IL-5 in nasal lavage fluid and OVA sIgE in peripheral serum were lower in the control group than in the model group.The number of eosinophils in local eosinophils decreased, and there was significant difference among the three groups. There was no significant difference in the concentrations of IFN 緯 -IL-10 and TSLP in nasal lavage fluid between IL-22 group and model group.Conclusion: nasal drip of rm ~ + IL-22 can inhibit the immune response in mice with allergic rhinitis and may be a new method for the treatment of allergic rhinitis.Aim: to investigate the expression of EGFPgene of recombinant adeno-associated virus (rAAV1) rAAV2 and rAAV8 in nasal epithelial cells via nasal drip transduction in mice.Methods six female BALB/c mice aged 6 to 8 weeks were randomly divided into 3 groups with 2 mice in each group.Mice were given nasal drip with recombinant adeno-associated virus 1t2 and type 8 containing EGFP gene. The mice were killed at the 4th week. The complete nasal cavity of mice was obtained, and the frozen sections were fixed after decalcification.Then the expression of EGFP in nasal epithelial cells of three groups was observed under fluorescence microscope.Results the EGFP carried by 1: rAAV1 vector was successfully transfected and stably expressed at the 4th week. The EGFP of the other two types of recombinant adeno-associated virus was not expressed in the nasal mucosa of mice.ConclusionTwo one rAAV1 vector can be transfected into mouse nasal mucosal epithelial cells and stably express EGFP. it can be used as a gene therapy vector for nasal diseases.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R765.21

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相關(guān)期刊論文 前1條

1 黃琨;劉厚君;涂亞庭;;尋常型銀屑病患者皮損中白介素-22和S100A7,A8,A9mRNA的表達(dá)及關(guān)系[J];中國(guó)皮膚性病學(xué)雜志;2007年03期

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