本文選題：醫(yī)學光學　切入點：藥物跨膜轉(zhuǎn)運　出處：《中國激光》2014年05期

【摘要】：利用激光掃描共聚焦顯微鏡動態(tài)研究大黃素β-環(huán)糊精包合物在鼻咽癌CNE-1細胞的跨膜轉(zhuǎn)運及分布。結(jié)果顯示,大黃素β-環(huán)糊精包合物以顆粒的形式主要分布于胞漿中,在其濃度為5~40mg·L-1時細胞對其攝取量隨濃度的增加呈非線性增加;NaN3、甘露醇可抑制CNE-1細胞對大黃素β-環(huán)糊精包合物的攝入量,環(huán)孢菌素A(CsA)在藥物濃度低(小于5mg·L-1)時可顯著增加細胞對大黃素β-環(huán)糊精包合物的攝入量,而在藥物濃度大時,反而抑制細胞的攝取量。相對大黃素而言,大黃素β-環(huán)糊精包合物在細胞內(nèi)持續(xù)時間較長。大黃素β環(huán)糊精包合物在鼻咽癌CNE-1細胞的跨膜轉(zhuǎn)運主要遵循能量依賴的內(nèi)吞模式且受P-gp蛋白的調(diào)控,這一特性可為藥物劑型研究提供參考。
[Abstract]:The transmembrane transport and distribution of emodin 尾 -cyclodextrin inclusion complex in nasopharyngeal carcinoma (NPC) CNE-1 cells were studied by laser scanning confocal microscopy. The results showed that emodin 尾 -cyclodextrin inclusion complex mainly distributed in the cytoplasm in the form of granules. When the concentration was 5~40mg L-1, the uptake of NAN3 was increased linearly with the increase of concentration. Mannitol inhibited the intake of emodin 尾 -cyclodextrin inclusion compound by CNE-1 cells. Cyclosporin A (CsA) significantly increased the intake of emodin 尾 -cyclodextrin inclusion complex when the drug concentration was lower than that of 5mg L-1, but inhibited the uptake of emodin 尾 -cyclodextrin when the drug concentration was high. The transmembrane transport of emodin 尾 -cyclodextrin inclusion complex in nasopharyngeal carcinoma (NPC) CNE-1 cells mainly follows an energy-dependent endocytosis pattern and is regulated by P-gp protein. This characteristic can be used as a reference for the study of drug formulation.
【作者單位】： 廣西醫(yī)科大學藥學院;廣西醫(yī)科大學醫(yī)學科學實驗中心;
【基金】：國家自然科學基金(81060270) 廣西醫(yī)學科學實驗中心開放基金(KFJJ2011-25)
【分類號】：R739.63

【參考文獻】

相關期刊論文 前7條

1 胡玲;張裕英;高長有;;聚合物納米粒子的結(jié)構(gòu)和性能對胞吞和細胞功能的影響[J];化學進展;2009年06期

2 葉宇煌;陳陽;李永增;馮尚源;蘇穎;鄒長h，

本文編號：1669424