本文選題：標(biāo)記滯留細(xì)胞　切入點(diǎn)：干細(xì)胞　出處：《廣州醫(yī)學(xué)院》2011年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：【目的】:利用5-溴脫氧尿嘧啶核苷(BrdU)標(biāo)記滯留細(xì)胞的方法研究小鼠喉粘膜和喉癌實(shí)體瘤內(nèi)可能存在的干細(xì)胞及其分布特點(diǎn)。 【方法】 出生3天的昆明小鼠36只,隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組,每組18只。實(shí)驗(yàn)組小鼠皮下注射BrdU 50μg/g,對(duì)照組皮下注射生理鹽水100μml,每天2次,共注射3d。于最后一次注射后2、4、8周分別處死6只小鼠,并取喉粘膜固定,石蠟包埋用于免疫組化檢測(cè),計(jì)算標(biāo)記滯留細(xì)胞的陽(yáng)性率。被BrdU標(biāo)記的喉癌Hep細(xì)胞注入6只裸鼠皮下成瘤,8周后處死并取腫瘤固定,石蠟包埋用于HE染色及免疫組化檢測(cè)。不同時(shí)間點(diǎn)標(biāo)記滯留細(xì)胞陽(yáng)性率的比較采用單因素方差分析,不同部位標(biāo)記滯留細(xì)胞陽(yáng)性率的比較采用最小顯著差數(shù)法。 【結(jié)果】 實(shí)驗(yàn)組標(biāo)記后2、4、8周,小鼠鱗狀上皮和腺體上皮均有標(biāo)記滯留細(xì)胞表達(dá),對(duì)照組標(biāo)記后無(wú)標(biāo)記滯留細(xì)胞表達(dá)。不同時(shí)間點(diǎn)標(biāo)記滯留細(xì)胞陽(yáng)性率差異具有顯著性(F=140.319,208.382 ;P=0.000,0.000)。鱗狀上皮基底層中標(biāo)記滯留細(xì)胞陽(yáng)性率顯著高于腺體細(xì)胞(t=6.376,10.944,15.376; P=0.000,0.000,0.000)。 【結(jié)論】 小鼠喉粘膜和喉癌實(shí)體瘤中存在標(biāo)記滯留細(xì)胞,并隨著觀(guān)察時(shí)間的延長(zhǎng)而逐漸減少,這些經(jīng)過(guò)長(zhǎng)時(shí)間觀(guān)察而標(biāo)記仍然存在的細(xì)胞被認(rèn)為是喉粘膜成體干細(xì)胞及喉癌腫瘤干細(xì)胞。
[Abstract]:Objective: to study the possible existence and distribution of stem cells in mouse laryngeal mucosa and laryngeal carcinoma solid tumor by using 5-bromodeoxyuridine BrdU labeling method. [methods]. Thirty-six Kunming mice were randomly divided into experimental group (n = 18) and control group (n = 18). Mice in the experimental group were subcutaneously injected with BrdU 50 渭 g / g, and the control group were subcutaneously injected with 100 渭 ml of normal saline twice a day. Six mice were killed at 2 and 4 weeks after the last injection, and the laryngeal mucosa was fixed with paraffin embedded for immunohistochemical examination. BrdU labeled laryngeal carcinoma Hep cells were injected into 6 nude mice subcutaneously to form tumor cells for 8 weeks, and the tumor was fixed. Paraffin embedding was used for HE staining and immunohistochemical detection. The positive rate of labeled retained cells at different time points was compared by univariate ANOVA and the least significant difference method was used to compare the positive rates of labeled retained cells in different sites. [results]. In the experimental group, the labeled residual cells were expressed in the squamous epithelium and glandular epithelium of the mice at 2 and 4 weeks after labeling. In the control group, the positive rate of labeled retained cells was significantly different at different time points. The positive rate of labeled retained cells in the basal layer of squamous epithelium was significantly higher than that in the basal layer of squamous epithelium, and the positive rate of labeled cells in the basal layer of squamous epithelium was significantly higher than that in the glandular cells. [conclusion]. In mice laryngeal mucous membrane and laryngeal carcinoma solid tumor, the number of labeled residual cells decreased gradually with the prolongation of observation time. These cells, which have been observed for a long time and still exist, are believed to be laryngeal mucosal adult stem cells and laryngeal cancer tumor stem cells.
【學(xué)位授予單位】：廣州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R739.65

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本文編號(hào)：1632500