本文選題：睡眠呼吸暫停　切入點(diǎn)：阻塞性　出處：《南京醫(yī)科大學(xué)》2010年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目的探討慢性間歇性缺氧(chronic intermittent hypoxia,CIH)對(duì)頦舌肌肌電、超微結(jié)構(gòu)及血清脂聯(lián)素水平的影響,并觀察補(bǔ)充脂聯(lián)素后有無(wú)干預(yù)效果。 方法健康雄性Wistar大鼠42只,隨機(jī)數(shù)字表法分為健康對(duì)照組(A組)、慢性間歇性缺氧組(B組)和脂聯(lián)素干預(yù)組(C組),每組14只。對(duì)B組、C組大鼠間歇缺氧(每日8 h,連續(xù)5周);同時(shí)C組大鼠給予注射用脂聯(lián)素10μg/次,A組與B組給予無(wú)菌生理鹽水0.5 ml/次,頸靜脈注射,每周2次,持續(xù)5周。第6周初采用插入式雙極針電極引導(dǎo)大鼠頦舌肌肌電,檢測(cè)各組動(dòng)物在低氧刺激前、低氧刺激和低氧刺激終止后各時(shí)間段的頦舌肌平均肌電電壓;以透射電鏡觀察頦舌肌肌細(xì)胞超微結(jié)構(gòu)的改變。 結(jié)果B組大鼠的血清脂聯(lián)素質(zhì)量濃度(1226.0±112.0) ng/ml顯著低于A組(2491.8±117.9)ng/ml,(q=38.2,P0.01);C組大鼠的血清脂聯(lián)素質(zhì)量濃度(1988.3±114.7)ng/ml較B組顯著增高(q=23.0,P0.01)。在低氧刺激前的基線狀態(tài)時(shí),B組頦舌肌肌電電壓水平(81.3±9.4)μV明顯低于A組(105.4±11.3)μV和C組(98.8±10.9)μV (q值分別為8.0、5.8,P值均0.01);低氧刺激5 min,A、B、C三組頦舌肌肌電電壓較基線時(shí)明顯增高(P值均0.01),其增高幅度A組最高,B組最低,C組居中,差異均有統(tǒng)計(jì)學(xué)意義(qab=17.5;qac=8.9;qbc=8.6,P值均0.01);低氧刺激終止后的15 min、30 min及45 min,A、C兩組頦舌肌電壓仍維持在較高的水平,顯著高于B組(P值均0.001)。CIH還造成頦舌肌結(jié)構(gòu)的變性如肌原纖維結(jié)構(gòu)紊亂,肌絲溶解、消失,線粒體水腫、嵴斷裂,部分線粒體空泡改變或溶解消失,而脂聯(lián)素可在一定程度上減輕這些病理改變。 結(jié)論CIH可引起頦舌肌病理改變及肌電活動(dòng)下降,該變化可能與CIH所致的低脂聯(lián)素血癥有關(guān)。
[Abstract]:Objective to investigate the effects of chronic intermittent hypoxia (intermittent) on genioglossus myoelectricity, ultrastructure and serum adiponectin levels, and to observe the effect of adiponectin supplementation. Methods 42 healthy male Wistar rats were used. Randomly divided into healthy control group A group, chronic intermittent hypoxia group B group) and adiponectin intervention group with 14 rats in each group. Rats in group B were subjected to intermittent hypoxia (8 hours per day for 5 weeks), while rats in group C were given intermittent hypoxia for 5 weeks. Adiponectin 10 渭 g / time group A and group B were given aseptic saline for 0.5 ml/. At the beginning of the sixth week, the genioglossus electromyography was guided by inserted bipolar needle electrode, and the genioglossus myoelectric activity was detected before hypoxia stimulation. The mean electromyoelectric voltage of genioglossus muscle was observed by transmission electron microscope and the ultrastructure of genioglossus muscle cells was observed by transmission electron microscope. Results the serum lipid diathesis concentration (1226.0 鹵112.0) ng/ml in group B was significantly lower than that in group A (2491.8 鹵117.9ng / ml). The serum lipid diathesis concentration in group C was significantly higher than that in group C (1988.3 鹵114.7ng / ml vs B). The electromyoelectric voltages of genioglossus in group B were significantly higher than those in group B before hypoxia. The Q values of the genioglossus in group A were significantly lower than those in group A (105.4 鹵11.3) 渭 V and in group C (98.8 鹵10.9) 渭 V (P = 0.01), respectively, and the electromyoelectric voltage of genioglossus in group C was significantly higher than that in group A (P = 0.01) after 5 min hypoxia stimulation, and the median value in group A was the highest in group A and the lowest in group B (P < 0.05). The difference was statistically significant (P < 0.01), and the voltage of genioglossus in groups 15 to 30 min and 45 min after hypoxia was still high, which was significantly higher than that in group B (P = 0.001.CIH), and it also caused the degeneration of genioglossus muscle structure such as myofibrillar structure disorder, which was significantly higher than that in group B (P = 0.001. CIH), and was significantly higher than that in group B (P = 0.001. CIH). Myofilament dissolved, disappeared, mitochondria edema, cristal rupture, partial mitochondrial vacuolation or dissolution disappeared, but adiponectin could alleviate these pathological changes to some extent. Conclusion CIH can induce the pathological changes and the decrease of myoelectric activity in genioglossus, which may be related to hypoadiponectinemia induced by CIH.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R766

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