本文選題：喉鱗狀細(xì)胞癌　切入點(diǎn)：局部復(fù)發(fā)　出處：《中國腫瘤臨床》2014年16期 　論文類型：期刊論文

【摘要】：目的:研究早期喉癌手術(shù)切緣陰性組織中p53、p21及PCNA表達(dá)的相關(guān)性及其與腫瘤復(fù)發(fā)的關(guān)系。方法:選取唐山市協(xié)和醫(yī)院2004年1月至2010年12月間92例早期喉癌患者為研究對象,采用免疫組織化學(xué)法聯(lián)合檢測腫瘤及其切緣組織中p53、p21及PCNA的表達(dá)情況。電話隨訪2年以上,對患者生存及腫瘤復(fù)發(fā)情況進(jìn)行觀察。結(jié)果:p53、p21及PCNA蛋白在喉癌組織中的表達(dá)與腫瘤的分級及分期無關(guān)。92例喉癌患者術(shù)后2年內(nèi)有16例出現(xiàn)復(fù)發(fā),復(fù)發(fā)率為17.39%。喉癌陰性切緣復(fù)發(fā)和未復(fù)發(fā)標(biāo)本中p53、p21及PCNA蛋白表達(dá)陽性的切緣復(fù)發(fā)率分別為50.00%、34.21%和33.33%,明顯高于p53、p21及PCNA蛋白表達(dá)陰性的切緣(8.33%、5.56%和9.68%)。經(jīng)統(tǒng)計學(xué)分析,喉癌復(fù)發(fā)標(biāo)本3種蛋白切緣陽性率明顯高于未復(fù)發(fā)標(biāo)本,均有顯著性差異(P0.01)。此外,喉癌陰性切緣組織中p53與p21蛋白的表達(dá)有明顯的相關(guān)性,PCNA的表達(dá)與p21的表達(dá)亦有明顯的相關(guān)性。結(jié)論:早期喉癌手術(shù)病理陰性切緣組織中的p53、p21及PCNA是患者預(yù)后的重要生物學(xué)標(biāo)志,聯(lián)合檢測喉癌切緣組織中p53、p21及PC-NA的表達(dá)有望作為預(yù)測喉癌復(fù)發(fā)的指標(biāo)。
[Abstract]:Objective: to study the relationship between the expression of p53 p21 and PCNA and the recurrence of early laryngeal carcinoma in the negative tissues of surgical margin of early laryngeal carcinoma. Methods: 92 patients with early laryngeal cancer were selected from January 2004 to December 2010 in Xuehe Hospital of Tangshan City. Immunohistochemical method was used to detect the expression of p53, p21 and PCNA in the tumor and its incisal margin. The patients were followed up by telephone for more than 2 years. Results the expression of% p53, p21 and PCNA protein in laryngeal carcinoma was not related to the grade and stage of the tumor. 16 cases of laryngeal carcinoma recurred within 2 years after operation. The recurrence rate of p53-p21 and PCNA protein positive margin in laryngeal cancer negative margin recurrence and non-recurrence specimens were 50.0034. 21% and 33. 33%, respectively, which were significantly higher than that of p53 p21 and PCNA protein negative margin 8. 33% and 9. 68%, respectively. The positive rate of the three proteins in recurrent laryngeal cancer specimens was significantly higher than that in non-recurrent specimens, and the difference was significant (P 0.01). The expression of p53 and p21 protein was significantly correlated with the expression of p21 protein in the negative margin of laryngeal carcinoma. Conclusion: the expression of p53 and p21 and PCNA in the negative margin tissue of early laryngeal carcinoma are prognostic. Important biological markers, The expression of p53 p21 and PC-NA in laryngeal carcinoma incised margin may be used as a predictor of laryngeal cancer recurrence.
【作者單位】： 唐山市協(xié)和醫(yī)院病理科;唐山市協(xié)和醫(yī)院耳鼻咽喉頭頸外科;
【分類號】：R739.65
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本文編號：1605517