本文關(guān)鍵詞： 鼻咽癌 高危人群 EBV-DNA 鼻咽癌篩查　出處：《中華腫瘤防治雜志》2014年17期 　論文類型：期刊論文

【摘要】：目的:探討鼻咽癌高危人群E病毒DNA(Epstein-Barr virus DNA,EBV-DNA)的存在情況及其在鼻咽癌早期診斷中的應(yīng)用價值。方法:2009-08-13-2010-07-27在廣東省中山市小欖鎮(zhèn)開展鼻咽癌篩查,ELISA法檢測16 712名EBV抗體,確定鼻咽癌高危人群386名,同時收集鼻咽癌低危人群273名。收集同期中山市人民醫(yī)院初診鼻咽癌患者62例。熒光定量PCR方法檢測高危人群血漿EBV-DNA,并隨訪1年,比較分析高危人群EBV-DNA定量檢測的應(yīng)用價值。結(jié)果:初篩鼻咽癌高危人群EBV-DNA陽性率為12.2%(47/386),高于鼻咽癌低危人群的3.3%(9/273),而低于初診鼻咽癌患者的91.9%(57/62),3組差異有統(tǒng)計學(xué)意義,P0.001。隨訪復(fù)查時,血清學(xué)持續(xù)高危人群EBV-DNA陽性率為9.7%(18/186),而血清學(xué)轉(zhuǎn)變?yōu)榉歉呶Ｈ巳旱腅BV-DNA陽性率為3.1%(3/97),差異有統(tǒng)計學(xué)意義,P=0.045。篩查人群中血清學(xué)診斷鼻咽癌的陽性預(yù)測值為5.6%(32/572),而在鼻咽癌高危人群中增加EBV-DNA檢測,其陽性預(yù)測值提高到44.6%(29/65)。結(jié)論:鼻咽癌高危人群血漿EBV-DNA定量檢測能對血清學(xué)EBV抗體檢測進行有效的補充,可大大提高篩查準(zhǔn)確性,對鼻咽癌高危人群的監(jiān)測具有重要作用。
[Abstract]:Objective: to investigate the presence of E virus DNA(Epstein-Barr virus DNA (EBV-DNA) in high risk population of nasopharyngeal carcinoma (NPC) and its value in early diagnosis of nasopharyngeal carcinoma. Methods: 16 712 EBV antibodies were detected by Elisa in Xiaolan Town, Zhongshan City, Guangdong Province. 386 cases of high risk population of nasopharyngeal carcinoma and 273 cases of low risk population of nasopharyngeal carcinoma were collected. In the same period, 62 cases of nasopharyngeal carcinoma were first diagnosed in Zhongshan people's Hospital. The plasma EBV-DNA was detected by fluorescence quantitative PCR and followed up for 1 year. Results: the positive rate of EBV-DNA in high risk population of nasopharyngeal carcinoma was 12.2%, which was significantly higher than that of low risk group (3.33% / 273), but lower than that of group 91.9% / 62% in nasopharyngeal carcinoma (NPC). There was a significant difference (P0.001) in the follow-up, and the positive rate of EBV-DNA in the high risk population of nasopharyngeal carcinoma (NPC) was 12.2% (P < 0.05), which was significantly higher than that in the low risk group (P < 0.01). The positive rate of EBV-DNA was 9. 7 / 18 / 1862 in the population with continuous high risk of serology, while the positive rate of EBV-DNA in the serological transition to non high risk group was 3. 1 / 97, the difference was statistically significant (P 0. 045). The positive predictive value of serological diagnosis of nasopharyngeal carcinoma in the screening population was 5. 6 / 572 and was high in nasopharyngeal carcinoma (NPC). Increased EBV-DNA detection in people at risk, Conclusion: the quantitative detection of plasma EBV-DNA in high risk population of nasopharyngeal carcinoma can effectively supplement the detection of serological EBV antibody, improve the accuracy of screening, and play an important role in the monitoring of high risk population of nasopharyngeal carcinoma.
【作者單位】： 中山大學(xué)附屬中山醫(yī)院腫瘤研究所;中山大學(xué)腫瘤防治中心·華南腫瘤學(xué)國家重點實驗室實驗研究部;
【基金】：“十一五”國家科技支撐計劃(2006BAI02A11) 中山市科技計劃(20083A183)
【分類號】：R739.63
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本文編號：1548629