【摘要】：背景:神經(jīng)干細胞具有多向分化的潛能及自我維持和更新能力,其遷徙能力強大,補腎活血中藥可減輕神經(jīng)元損傷及促進神經(jīng)細胞再生修復(fù),實現(xiàn)神經(jīng)功能重建,補腎活血方聯(lián)合神經(jīng)干細胞應(yīng)用于水楊酸鈉耳鳴大鼠模型的作用機制尚不清楚。目的:探討補腎活血方聯(lián)合神經(jīng)干細胞對耳鳴大鼠模型的影響。方法:取SD大鼠60只隨機等分為5組,正常對照組、模型組、補腎活血方組、神經(jīng)干細胞組和聯(lián)合組,后4組建立水楊酸鈉耳鳴大鼠模型,造模15 d后,補腎活血方單組每天灌胃3 mL灌胃補腎活血方水煎液(2.592 g/mL),連續(xù)7 d,神經(jīng)干細胞組尾靜脈注射1 m L神經(jīng)干細胞培養(yǎng)液(1.0×109 L-1);聯(lián)合組則先通過混懸液灌胃給藥后再進行尾靜脈注射神經(jīng)干細胞培養(yǎng)液。結(jié)果與結(jié)論:(1)補腎活血方和/或神經(jīng)干細胞治療均能有效促進大鼠飲水抑制率的恢復(fù),且飲水抑制率:聯(lián)合組補腎活血方組神經(jīng)干細胞組;(2)補腎活血方和/或神經(jīng)干細胞治療均能有效減輕大腦初級聽皮質(zhì)的病理損傷;(3)Western blot結(jié)果表補腎活血方組及聯(lián)合組大鼠大鼠5-羥色胺表達水平較模型組增加,且補腎活血方組與聯(lián)合組大鼠腦組織5-羥色胺表達水平高于神經(jīng)干細胞組;(4)ELISA法檢測結(jié)果與Western blot結(jié)果一致;(5)表明補腎活血方聯(lián)合神經(jīng)干細胞對水楊酸鈉耳鳴大鼠模型有一定療效,其作用機制可能與修復(fù)神經(jīng)元損傷及5-羥色胺含量及其蛋白表達有關(guān)。
[Abstract]:Background: neural stem cells have the potential of multidirectional differentiation and the ability of self-sustainment and renewal, and their migration ability is strong. Traditional Chinese medicine for tonifying kidney and activating blood circulation can reduce neuron injury, promote nerve cell regeneration and repair, and realize neural function reconstruction. The mechanism of Bushen Huoxue recipe combined with neural stem cells in sodium salicylate tinnitus rat model is not clear. Objective: to investigate the effect of Bushen Huoxue recipe combined with neural stem cells on tinnitus rat model. Methods: 60 SD rats were randomly divided into 5 groups: normal control group, model group, Bushen Huoxue recipe group, neural stem cell group and combined group. The rat model of sodium salicylate tinnitus was established in the latter four groups. 15 days later, the single group of Bushen Huoxue recipe was intragastrically perfused with Bushen Huoxue recipe decoction (2.592 g/mL) for 7 days. In the neural stem cell group, 1 mL neural stem cell culture medium (1.0 脳 109 L 鈮，

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