發(fā)布時間：2019-02-13 13:44

【摘要】：目的探討鐵碳納米微粒荷載順鉑對喉癌Hep-2細(xì)胞的增殖抑制作用的影響及其作用機制。 方法選擇喉癌Hep-2細(xì)胞進行復(fù)蘇及傳代，對數(shù)生長期Hep-2細(xì)胞分為對照組、微粒組、藥物組及微粒藥物組，各組分別加入處理液，對照組是生理鹽水，藥物組的處理液為順鉑生理鹽水溶液，微粒藥物組為鐵碳納米粒順鉑生理鹽水分散液，微粒組是鐵碳納米微粒生理鹽水分散液。細(xì)胞培養(yǎng)24及48h后計算各組細(xì)胞的生長抑制率，細(xì)胞培養(yǎng)3天后觀察細(xì)胞形態(tài)，細(xì)胞培養(yǎng)5天后采用RT-PCR技術(shù)檢測各組細(xì)胞Caspase3mRNA及Survivin mRNA的表達情況，比較各組細(xì)胞生長抑制率，細(xì)胞生長形態(tài)及各組細(xì)胞Caspase3mRNA及Survivin mRNA的表達水平的差異。 結(jié)果各組細(xì)胞加入處理液培養(yǎng)24小時后，對照組、微粒組、藥物組及微粒藥物組細(xì)胞生長抑制率分別為4.1%、4.7%、19.6%和25.6%，微粒藥物組及藥物組細(xì)胞生長抑制率高于對照組及微粒組，微粒藥物組生長抑制率高于藥物組，對照組同微粒組生長抑制率無顯著差異。各組細(xì)胞培養(yǎng)48小時后，對照組、微粒組、藥物組及微粒藥物組細(xì)胞生長抑制率分別為4.0%、4.5%、37.2%和51.4%，微粒藥物組及藥物組細(xì)胞生長抑制率高于對照組及微粒組，微粒藥物組生長抑制率高于藥物組，對照組同微粒組生長抑制率無顯著差異。細(xì)胞培養(yǎng)3天后，微粒藥物組及藥物組細(xì)胞生長抑制，表現(xiàn)為細(xì)胞生長不貼壁，增殖緩慢，出現(xiàn)凋亡征象，微粒藥物組細(xì)胞生長抑制較藥物組更為明顯，各組喉癌Hep-2細(xì)胞培養(yǎng)5天后RT-PCR檢測Caspase3mRNA和Survivin mRNA表達水平結(jié)果顯示，對照組、微粒組、藥物組及微粒藥物組細(xì)胞Caspase3mRNA表達水平分別為0.84±0.14、0.87±0.16、1.41±0.25和1.93±0.27，藥物組及微粒藥物組細(xì)胞Caspase3mRNA表達水平高于微粒組及對照組，，微粒藥物組細(xì)胞Caspase3mRNA表達水平高于藥物組，對照組、微粒組、藥物組及微粒藥物組細(xì)胞SurvivinmRNA表達水平水平分別為1.34±0.17、1.29±0.13、0.84±0.19和0.52±0.14，藥物組及微粒藥物組細(xì)胞Survivin mRNA表達水平低于對照組及微粒組，微粒藥物組細(xì)胞Survivin mRNA表達水平低于藥物組，微粒組同對照組Caspase3mRNA、Survivin mRNA表達水平無顯著差異。 結(jié)論鐵碳納米微粒搭載順鉑能夠增加喉癌Hep-2細(xì)胞Caspase3mRNA的表達，降低Survivin mRNA的表達，增加細(xì)胞生長抑制率，增加喉癌Hep-2細(xì)胞對順鉑的敏感性，提高藥物化療效果。
[Abstract]:Objective to investigate the effect of iron carbon nanoparticles loading cisplatin on the proliferation inhibition of laryngeal carcinoma Hep-2 cells and its mechanism. Methods Hep-2 cells of laryngeal carcinoma were selected for resuscitation and passage. Hep-2 cells in logarithmic growth phase were divided into control group, microparticle group, drug group and microparticle drug group. The treatment solution of drug group was cisplatin normal saline solution, the particle drug group was iron carbon nanoparticles cisplatin normal saline dispersion, and the particle group was iron carbon nanoparticles normal saline dispersion. After 24 and 48 hours of cell culture, the growth inhibition rate of each group was calculated, the cell morphology was observed after 3 days of cell culture, the expression of Caspase3mRNA and Survivin mRNA was detected by RT-PCR technique after 5 days of cell culture, and the growth inhibition rate of each group was compared. The difference of cell growth morphology and the expression of Caspase3mRNA and Survivin mRNA in each group. Results after the cells were cultured in the treatment solution for 24 hours, the cell growth inhibition rates of the control group, the microparticle group, the drug group and the microparticle drug group were 4.1% and 25.6%, respectively. The cell growth inhibition rate of the microparticle drug group and the drug group was higher than that of the control group and the microparticle group, and the growth inhibition rate of the particle drug group was higher than that of the drug group, but there was no significant difference between the control group and the particle group. After 48 hours of culture, the cell growth inhibition rates of control group, microparticle group, drug group and microparticle drug group were 4.0% and 51.4%, respectively. The cell growth inhibition rate of the microparticle drug group and the drug group was higher than that of the control group and the microparticle group, and the growth inhibition rate of the particle drug group was higher than that of the drug group, but there was no significant difference between the control group and the particle group. After 3 days of cell culture, cell growth inhibition was observed in the microparticle drug group and the drug group, showing that cell growth was not adhered to the wall, proliferation was slow, and apoptosis was observed. The cell growth inhibition in the microparticle drug group was more obvious than that in the drug group. The expression levels of Caspase3mRNA and Survivin mRNA in Hep-2 cells of laryngeal carcinoma were detected by RT-PCR 5 days later. The results showed that the expression of Caspase3mRNA and Survivin mRNA in the control group and the microparticle group were measured. The expression levels of Caspase3mRNA were 0.84 鹵0.140.87 鹵0.16 鹵0.41 鹵0.25 and 1.93 鹵0.27 in drug group and microparticle drug group, respectively. The expression level of Caspase3mRNA in drug group and microparticle drug group was higher than that in microparticle group and control group. The expression level of Caspase3mRNA in the microparticle drug group was higher than that in the drug group. The SurvivinmRNA expression levels in the control group, the microparticle group, the drug group and the microparticle drug group were 1.34 鹵0.17, 1.29 鹵0.13, 0.84 鹵0.19 and 0.52 鹵0.14, respectively. The expression level of Survivin mRNA in the drug group and microparticle group was lower than that in the control group and microparticle group. The expression level of Survivin mRNA in the microparticle drug group was lower than that in the drug group. There was no significant difference between the microparticle group and the control group in the expression of Caspase3mRNA,Survivin mRNA. Conclusion Cisplatin in iron carbon nanoparticles can increase the expression of Caspase3mRNA, decrease the expression of Survivin mRNA, increase the growth inhibition rate, increase the sensitivity of Hep-2 cells to cisplatin, and improve the effect of drug chemotherapy.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R739.65

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