Tau在鼻咽癌親本及紫杉醇耐藥細胞系中的表達差異
發(fā)布時間：2018-09-11 18:16
【摘要】：目的： 分析微管相關(guān)蛋白Tau在鼻咽癌親本細胞及紫杉醇耐藥細胞系中的表達差異,探討Tau在鼻咽癌紫杉醇耐藥中的作用。 方法： 以鼻咽癌親本細胞株CNE-1、CNE-2及其相對應的紫杉醇耐藥細胞株CNE-1/taxol、CNE-2/taxol為實驗對象,分別應用Western Bolt及實時熒光定量PCR技術(shù)檢測Tau在這些細胞中的蛋白和mRNA水平的表達。 結(jié)果： 以Western Bolt和實時熒光定量PCR的結(jié)果反映Tau在不同細胞系中蛋白和mRNA水平的表達。Tau蛋白的表達水平以相對光密度參考值,即Tau蛋白的光密度參考值/同一樣本GAPDH的光密度參考值表示。mRNA的相對表達量用2-△Ct方法計算得出。結(jié)果得出,Tau蛋白在鼻咽癌親本及紫杉醇耐藥細胞系中均有表達,其中耐藥細胞CNE-1/taxol、CNE-2/taxol分別是親本細胞CNE-1、CNE-2的3.11±0.54,1.39±0.21倍。Tau的mRNA在各個鼻咽癌細胞中也有表達,耐藥細胞CNE-1/taxol、CNE-2/taxol分別是親本細胞CNE-1、CNE-2的2.48±0.33、1.67±0.15倍。 結(jié)論： Tau在鼻咽癌紫杉醇耐藥細胞系中的表達高于親本細胞系,Tau可能與紫杉醇耐藥相關(guān)。Tau可以作為紫杉醇化療敏感性及預后的個預測分子,對其進行干預可以在一定程度上逆轉(zhuǎn)紫杉醇耐藥。
[Abstract]:Aim: to investigate the expression of microtubule-associated protein (Tau) in nasopharyngeal carcinoma (NPC) parent cells and paclitaxel resistant cell lines, and to explore the role of Tau in taxol resistance of nasopharyngeal carcinoma (NPC). Methods: the expression of Tau protein and mRNA in nasopharyngeal carcinoma (NPC) parent cell line CNE-1,CNE-2 and its taxol resistant cell line CNE-1/taxol,CNE-2/taxol were detected by Western Bolt and real-time fluorescence quantitative PCR. Results: the results of Western Bolt and real-time quantitative PCR showed that the expression level of Tau protein and mRNA in different cell lines was the reference value of relative optical density. The optical density reference value of Tau protein and the optical density reference value of GAPDH in the same sample were calculated by 2-Ct method. The results showed that CNE-1/taxol,CNE-2/taxol protein was expressed in nasopharyngeal carcinoma (NPC) parents and paclitaxel resistant cell lines. The expression of mRNA in resistant cells was 3.11 鹵0.54n1.39 鹵0.21 times of parental CNE-1,CNE-2, respectively. Tau was also expressed in various nasopharyngeal carcinoma cells. The CNE-1/taxol,CNE-2/taxol of drug resistant cells was 2.48 鹵0.33 1.67 鹵0.15 times of that of parental CNE-1,CNE-2, respectively. Conclusion: the expression of Tau in paclitaxel-resistant nasopharyngeal carcinoma cell line is higher than that in parent cell line. Tau may be a predictor of paclitaxel chemosensitivity and prognosis. Intervention can reverse paclitaxel resistance to some extent.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R739.63
本文編號：2237481
[Abstract]:Aim: to investigate the expression of microtubule-associated protein (Tau) in nasopharyngeal carcinoma (NPC) parent cells and paclitaxel resistant cell lines, and to explore the role of Tau in taxol resistance of nasopharyngeal carcinoma (NPC). Methods: the expression of Tau protein and mRNA in nasopharyngeal carcinoma (NPC) parent cell line CNE-1,CNE-2 and its taxol resistant cell line CNE-1/taxol,CNE-2/taxol were detected by Western Bolt and real-time fluorescence quantitative PCR. Results: the results of Western Bolt and real-time quantitative PCR showed that the expression level of Tau protein and mRNA in different cell lines was the reference value of relative optical density. The optical density reference value of Tau protein and the optical density reference value of GAPDH in the same sample were calculated by 2-Ct method. The results showed that CNE-1/taxol,CNE-2/taxol protein was expressed in nasopharyngeal carcinoma (NPC) parents and paclitaxel resistant cell lines. The expression of mRNA in resistant cells was 3.11 鹵0.54n1.39 鹵0.21 times of parental CNE-1,CNE-2, respectively. Tau was also expressed in various nasopharyngeal carcinoma cells. The CNE-1/taxol,CNE-2/taxol of drug resistant cells was 2.48 鹵0.33 1.67 鹵0.15 times of that of parental CNE-1,CNE-2, respectively. Conclusion: the expression of Tau in paclitaxel-resistant nasopharyngeal carcinoma cell line is higher than that in parent cell line. Tau may be a predictor of paclitaxel chemosensitivity and prognosis. Intervention can reverse paclitaxel resistance to some extent.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R739.63
【參考文獻】
相關(guān)期刊論文 前2條
1 張力,姜文奇,徐瑞華,管忠震;紫杉醇(Paclitaxel)治療晚期鼻咽癌的臨床研究[J];癌癥;2000年08期
2 丁有奕;黃渚;王延紅;;紫杉醇治療肺癌48例不良反應分析[J];海南醫(yī)學;2007年07期
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