IL-16、IL-32在前葡萄膜炎患者血清中的表達(dá)及意義
發(fā)布時間：2018-07-23 15:54
【摘要】：目的:1觀察本研究中葡萄膜炎患者的臨床特點、治療效果、視力預(yù)后、并發(fā)癥以及診治過程中存在的問題。2檢測IL-16和IL-32在正常人及前葡萄膜炎患者血清中的表達(dá)情況,比較組間IL-16和IL-32表達(dá)水平有無明顯差異,分別分析它們與疾病之間的相關(guān)性。3檢測IL-16和IL-32在前葡萄膜炎活動期及穩(wěn)定期患者血清中的表達(dá)情況,比較其表達(dá)水平有無差異,并檢測IL-16和IL-32在前葡萄膜炎患者治療前后血清中的表達(dá)情況,比較治療前后有無明顯差異,進(jìn)一步分析它們與疾病的關(guān)系。4探討IL-16和IL-32在前葡萄膜炎發(fā)病中的作用,尋找治療前葡萄膜炎的新方法和新藥物。方法:1收集自2015年1月至2017年1月于河北醫(yī)科大學(xué)第一醫(yī)院就診的各類葡萄膜炎患者85例,其中男45例,女40例,平均發(fā)病年齡(41.25±16.83),發(fā)病年齡范圍為7-70歲,詳細(xì)記錄他們的全身情況、眼病史以及臨床診療情況。同期收集非葡萄膜炎患者40例作為正常對照組,其中男22例,女18例,平均年齡(42.33±12.68)。所有對照者排除葡萄膜炎病史,既往身體健康,近期未服用過抗生素、糖皮質(zhì)激素、非甾體類激素和免疫抑制劑。2采集40例前葡萄膜炎實驗組患者及40例正常對照組患者外周靜脈血標(biāo)本4-6ml,離心后提取血清,然后應(yīng)用雙抗體夾心法酶聯(lián)免疫吸附試驗(ELISA),來檢測IL-16和IL-32在實驗組及正常對照組血清中的表達(dá)情況,并對實驗組及正常對照組行血沉(Erythrocyte Sedimentation Rate,ESR)、類風(fēng)濕因子(Rheumatoid factor,RF)、C反應(yīng)蛋白(C-Reactive protein,CRP)、抗鏈“O”等檢查,分別將所得檢測結(jié)果進(jìn)行統(tǒng)計學(xué)分析。結(jié)果:1本研究85例葡萄膜炎患者中,21-45歲患者最多,發(fā)病年齡范圍為7-70歲,男女比例為1.125:1。其中單眼發(fā)病患者67例,雙眼同時受累的患者有18例。按解剖部位來看前葡萄膜炎(64例)最多,其次全葡萄膜炎13例,中間葡萄膜炎及后葡萄膜炎各4例,根據(jù)病程來分急性葡萄膜炎患者(61例)最多,慢性葡萄膜炎患者5例,復(fù)發(fā)性葡萄膜炎患者19例,其中伴有強(qiáng)直性脊柱炎的患者為7例,伴有vogt-小柳-原田(vkh)綜合征的有6例(7.9%),伴有behcet病的為5例,合并fuchs綜合征4例。本研究85例研究對象中有31例出現(xiàn)白內(nèi)障、青光眼或眼壓升高、虹膜粘連等并發(fā)癥,大部分患者經(jīng)過及時的積極的藥物治療后視力都有所提高。2前葡萄膜炎組患者血清中il-16和il-32的濃度分別為196.42(140.77,263.26)和265.67(214.51,342.82);正常對照組血清il-16和il-32的濃度分別為82.68(49.03,115.38)和86.19(51.57,154.28)。前葡萄膜炎組血清il-16濃度與正常對照組比較差異有統(tǒng)計學(xué)意義(p=0.003),前葡萄膜炎組血清il-32濃度與正常對照組比較差異也有統(tǒng)計學(xué)意義(p=0.008)。3前葡萄膜炎活動期患者血清il-16水平209.55(152.89,277.40)較穩(wěn)定期水平97.19(57.92,123.17)升高,差異有統(tǒng)計學(xué)意義(p=0.000);活動期患者血清il-32水平278.98(222.12,346.80)較穩(wěn)定期患者95.48(68.26,167.64)升高,差異有統(tǒng)計學(xué)意義(p=0.024)。4前葡萄膜炎患者治療后血清il-16水平84.55(51.39,127.04)較治療前水平196.42(140.77,263.26)顯著降低,差異有統(tǒng)計學(xué)意義(p=0.004);并且前葡萄膜炎治療后血清il-32水平88.98(52.17,165.43)較治療前水平265.67(214.51,342.82)也顯著降低,差異也有統(tǒng)計學(xué)意義(p=0.000)。5用spearman相關(guān)分別分析il-16和il-32與前葡萄膜炎疾病的相關(guān)性,結(jié)果顯示il-16與疾病呈正相關(guān)(spearman相關(guān)系數(shù)r=0.671,p=0.000),il-32也與前葡萄膜炎呈正相關(guān)(spearman相關(guān)系數(shù)r=0.963,p=0.000)。il-16與il-32之間呈正相關(guān)(spearman相關(guān)系數(shù)r=0.383,p=0.009)。6前葡萄膜炎組患者esr、rf、crp、抗鏈“o”檢測結(jié)果分別是24.50(15.50,36.50)、6.30(4.85,9.80)、64.10(11.30,113.10)和4.80(4.02,5.47);正常對照組esr、rf、crp、抗鏈“o”檢測結(jié)果分別為15.60(6.90,27.70)、4.52(3.07,8.35)、47.50(8.10,95.80)和3.43(2.65,4.12)。前葡萄膜炎組患者ESR水平較正常對照組升高,差異有統(tǒng)計學(xué)意義(P=0.004)。兩組患者之間RF、CRP、抗鏈“O”水平差異無統(tǒng)計學(xué)意義(RF:P=0.645;CRP:P=0.053;抗鏈“O”:P=0.620)。7用Spearman相關(guān)分別分析IL-16、IL-32與前葡萄膜炎組患者年齡、ESR、RF、CRP和抗鏈“O”的相關(guān)性,結(jié)果未發(fā)現(xiàn)血清IL-16、IL-32與年齡、ESR、RF、CRP和抗鏈“O”的相關(guān)性(P0.05)。結(jié)論:1本研究中發(fā)現(xiàn)葡萄膜炎在中青年人群中發(fā)病多見,前葡萄膜炎為臨床常見類型,白內(nèi)障和青光眼或眼壓升高為常見并發(fā)癥,大部分患者視力預(yù)后較好,少數(shù)出現(xiàn)視力損害。2前葡萄膜炎組患者血清IL-16、IL-32水平分別與正常對照組比較明顯升高;IL-16、IL-32分別與疾病呈正相關(guān),證實了IL-16、IL-32是前葡萄膜炎中重要的細(xì)胞因子,表明IL-16、IL-32參與了前葡萄膜炎的發(fā)病。3前葡萄膜炎活動期患者血清IL-16、IL-32水平較穩(wěn)定期水平明顯升高,證實了IL-16、IL-32與前葡萄膜炎的活動有關(guān)。4前葡萄膜炎患者治療后血清IL-16、IL-32水平較治療前水平明顯降低,更進(jìn)一步證實了IL-16、IL-32參與了前葡萄膜炎的發(fā)生發(fā)展。5血清中IL-16和IL-32之間呈正相關(guān),認(rèn)為其分別或協(xié)同參與了前葡萄膜炎的發(fā)生和演進(jìn)。
[Abstract]:Objective: 1 to observe the clinical characteristics, therapeutic effect, visual prognosis, complications, and problems in the diagnosis and treatment of uveitis in this study,.2 detection of the expression of IL-16 and IL-32 in the serum of normal people and patients with anterior uveitis, and to compare the differences of IL-16 and IL-32 expression levels between groups and analyze them with the disease, respectively. The correlation.3 detected the expression of IL-16 and IL-32 in the sera of the active and stable patients with anterior uveitis, compared with the expression level, and detected the expression of IL-16 and IL-32 in the sera before and after the treatment of the patients with anterior uveitis. There was no significant difference before and after the treatment, and the relationship between them and the disease was further analyzed. .4 was used to explore the role of IL-16 and IL-32 in the pathogenesis of anterior uveitis, looking for new methods and new drugs for the treatment of pre uveitis. 1 a total of 85 cases of uveitis were collected from January 2015 to January 2017 in the first hospital of Hebei Medical University, including 45 males and 40 females, with an average age of onset (41.25 + 16.83), and the age range of onset. At the age of 7-70, the whole body condition, the history of ophthalmopathy and the clinical diagnosis and treatment were recorded in detail. 40 cases of non uveitis were collected in the same period as the normal control group, including 22 males and 18 females, with an average age of 42.33 + 12.68. All the controls excluded the history of uveitis, previous body health, no antibiotics, glucocorticoids, and non steroids. The body hormone and immunosuppressant.2 collected 40 cases of anterior uveitis and 40 cases of normal control group of peripheral venous blood samples 4-6ml, extracted serum after centrifugation, and then used the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the expression of IL-16 and IL-32 in the serum of the experimental group and the normal control group. The test group and the normal control group were Erythrocyte Sedimentation Rate (ESR), rheumatoid factor (Rheumatoid factor, RF), C reactive protein (C-Reactive protein, CRP) and anti chain O). The results were statistically analyzed. Results: 1 of the 85 cases of uveitis were the most 21-45 years old patients and age model. The peri was 7-70 years old, with a male and female ratio of 1.125:1. with 67 cases of monocular disease and 18 cases with double binocular involvement. According to the anatomical site, anterior uveitis (64 cases) was the most, followed by full uveitis in 13 cases, middle uveitis and 4 cases of posterior uveitis, according to the course of the disease (61 cases), the most, chronic uveitis There were 5 patients and 19 cases of recurrent uveitis, including 7 cases with ankylosing spondylitis, 6 cases (7.9%) with vogt- Xiao Liu Tian (VKH) syndrome, 5 cases with Behcet disease and 4 cases of Fuchs syndrome. In this study, 85 cases of the study included cataract, glaucoma or intraocular pressure, and synechia of the iris. The visual acuity of most patients after timely active drug treatment improved the concentration of IL-16 and IL-32 in the serum of.2 anterior uveitis group was 196.42 (140.77263.26) and 265.67 (214.51342.82), and the serum concentration of IL-16 and IL-32 in the normal control group was 82.68 (49.03115.38) and 86.19 (51.57154.28), respectively. The serum IL-16 concentration was significantly different from that of the normal control group (p=0.003). The serum IL-32 concentration in the anterior uveitis group was also significantly different from that of the normal control group (p=0.008) the serum IL-16 level 209.55 (152.89277.40) was higher than the stable level 97.19 (57.92123.17) in the patients with.3 anterior uveitis. Study significance (p=0.000); serum IL-32 level 278.98 (222.12346.80) of patients at active stage was higher than that of stable and regular patients (68.26167.64). The difference was statistically significant (p=0.024) in patients with.4 before uveitis, serum IL-16 level 84.55 (51.39127.04) was significantly lower than 196.42 (140.77263.26) before treatment (p=), and the difference was statistically significant (p=). 0.004); and the serum IL-32 level 88.98 (52.17165.43) after the treatment of anterior uveitis was also significantly lower than that before treatment (214.51342.82), and the difference was also statistically significant (p=0.000).5 with Spearman correlation analysis of IL-16 and IL-32 and anterior uveitis. The results showed that IL-16 was positively related to the disease (Spearman correlation). The coefficient r=0.671, p=0.000) and IL-32 were also positively correlated with anterior uveitis (Spearman correlation coefficient r=0.963, p=0.000).Il-16 and IL-32 (Spearman correlation coefficient r=0.383, p=0.009) before.6 anterior uveitis. The results were 24.50, 6.30, 64.10, and 4.80 respectively. (4.02,5.47); the results of ESR, RF, CRP and anti chain "O" in the normal control group were 15.60 (6.90,27.70), 4.52 (3.07,8.35), 47.50 (8.10,95.80) and 3.43 (2.65,4.12). The ESR level of the anterior uveitis group was higher than that in the normal control group, and the difference was statistically significant (P=0.004). There was no statistical difference between the two groups. Meaning (RF:P=0.645; CRP:P=0.053; anti chain "O": P=0.620).7 with Spearman correlation analysis of the correlation between IL-16, IL-32 and the patients with anterior uveitis, ESR, RF, CRP and anti chain O. Conclusion: the 1 studies found that uveitis is in middle green. The incidence of anterior uveitis is common in the population. The common complications of anterior uveitis are cataract and glaucoma or intraocular pressure. Most of the patients have better visual prognosis. There are few visual impairment in.2 anterior uveitis patients' serum IL-16, and the level of IL-32 is significantly higher than that in the normal control group; IL-16, IL-32 is positive with the disease, respectively. Correlation, confirmed that IL-16, IL-32 is an important cytokine in the anterior uveitis, indicating that IL-16, IL-32 participates in the serum IL-16 in the patients with anterior uveitis during the onset of anterior uveitis, and the level of IL-32 is significantly higher than that in the stable stage, which confirms that IL-16, IL-32 and the activity of anterior uveitis are related to the serum IL of the patients with.4 anterior uveitis after treatment. -16, IL-32 level was significantly lower than before treatment, and further confirmed IL-16, IL-32 participated in the occurrence and development of anterior uveitis,.5 serum was positively correlated with IL-16 and IL-32, which was considered to be involved in the occurrence and evolution of anterior uveitis respectively.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R773
本文編號：2139896
[Abstract]:Objective: 1 to observe the clinical characteristics, therapeutic effect, visual prognosis, complications, and problems in the diagnosis and treatment of uveitis in this study,.2 detection of the expression of IL-16 and IL-32 in the serum of normal people and patients with anterior uveitis, and to compare the differences of IL-16 and IL-32 expression levels between groups and analyze them with the disease, respectively. The correlation.3 detected the expression of IL-16 and IL-32 in the sera of the active and stable patients with anterior uveitis, compared with the expression level, and detected the expression of IL-16 and IL-32 in the sera before and after the treatment of the patients with anterior uveitis. There was no significant difference before and after the treatment, and the relationship between them and the disease was further analyzed. .4 was used to explore the role of IL-16 and IL-32 in the pathogenesis of anterior uveitis, looking for new methods and new drugs for the treatment of pre uveitis. 1 a total of 85 cases of uveitis were collected from January 2015 to January 2017 in the first hospital of Hebei Medical University, including 45 males and 40 females, with an average age of onset (41.25 + 16.83), and the age range of onset. At the age of 7-70, the whole body condition, the history of ophthalmopathy and the clinical diagnosis and treatment were recorded in detail. 40 cases of non uveitis were collected in the same period as the normal control group, including 22 males and 18 females, with an average age of 42.33 + 12.68. All the controls excluded the history of uveitis, previous body health, no antibiotics, glucocorticoids, and non steroids. The body hormone and immunosuppressant.2 collected 40 cases of anterior uveitis and 40 cases of normal control group of peripheral venous blood samples 4-6ml, extracted serum after centrifugation, and then used the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the expression of IL-16 and IL-32 in the serum of the experimental group and the normal control group. The test group and the normal control group were Erythrocyte Sedimentation Rate (ESR), rheumatoid factor (Rheumatoid factor, RF), C reactive protein (C-Reactive protein, CRP) and anti chain O). The results were statistically analyzed. Results: 1 of the 85 cases of uveitis were the most 21-45 years old patients and age model. The peri was 7-70 years old, with a male and female ratio of 1.125:1. with 67 cases of monocular disease and 18 cases with double binocular involvement. According to the anatomical site, anterior uveitis (64 cases) was the most, followed by full uveitis in 13 cases, middle uveitis and 4 cases of posterior uveitis, according to the course of the disease (61 cases), the most, chronic uveitis There were 5 patients and 19 cases of recurrent uveitis, including 7 cases with ankylosing spondylitis, 6 cases (7.9%) with vogt- Xiao Liu Tian (VKH) syndrome, 5 cases with Behcet disease and 4 cases of Fuchs syndrome. In this study, 85 cases of the study included cataract, glaucoma or intraocular pressure, and synechia of the iris. The visual acuity of most patients after timely active drug treatment improved the concentration of IL-16 and IL-32 in the serum of.2 anterior uveitis group was 196.42 (140.77263.26) and 265.67 (214.51342.82), and the serum concentration of IL-16 and IL-32 in the normal control group was 82.68 (49.03115.38) and 86.19 (51.57154.28), respectively. The serum IL-16 concentration was significantly different from that of the normal control group (p=0.003). The serum IL-32 concentration in the anterior uveitis group was also significantly different from that of the normal control group (p=0.008) the serum IL-16 level 209.55 (152.89277.40) was higher than the stable level 97.19 (57.92123.17) in the patients with.3 anterior uveitis. Study significance (p=0.000); serum IL-32 level 278.98 (222.12346.80) of patients at active stage was higher than that of stable and regular patients (68.26167.64). The difference was statistically significant (p=0.024) in patients with.4 before uveitis, serum IL-16 level 84.55 (51.39127.04) was significantly lower than 196.42 (140.77263.26) before treatment (p=), and the difference was statistically significant (p=). 0.004); and the serum IL-32 level 88.98 (52.17165.43) after the treatment of anterior uveitis was also significantly lower than that before treatment (214.51342.82), and the difference was also statistically significant (p=0.000).5 with Spearman correlation analysis of IL-16 and IL-32 and anterior uveitis. The results showed that IL-16 was positively related to the disease (Spearman correlation). The coefficient r=0.671, p=0.000) and IL-32 were also positively correlated with anterior uveitis (Spearman correlation coefficient r=0.963, p=0.000).Il-16 and IL-32 (Spearman correlation coefficient r=0.383, p=0.009) before.6 anterior uveitis. The results were 24.50, 6.30, 64.10, and 4.80 respectively. (4.02,5.47); the results of ESR, RF, CRP and anti chain "O" in the normal control group were 15.60 (6.90,27.70), 4.52 (3.07,8.35), 47.50 (8.10,95.80) and 3.43 (2.65,4.12). The ESR level of the anterior uveitis group was higher than that in the normal control group, and the difference was statistically significant (P=0.004). There was no statistical difference between the two groups. Meaning (RF:P=0.645; CRP:P=0.053; anti chain "O": P=0.620).7 with Spearman correlation analysis of the correlation between IL-16, IL-32 and the patients with anterior uveitis, ESR, RF, CRP and anti chain O. Conclusion: the 1 studies found that uveitis is in middle green. The incidence of anterior uveitis is common in the population. The common complications of anterior uveitis are cataract and glaucoma or intraocular pressure. Most of the patients have better visual prognosis. There are few visual impairment in.2 anterior uveitis patients' serum IL-16, and the level of IL-32 is significantly higher than that in the normal control group; IL-16, IL-32 is positive with the disease, respectively. Correlation, confirmed that IL-16, IL-32 is an important cytokine in the anterior uveitis, indicating that IL-16, IL-32 participates in the serum IL-16 in the patients with anterior uveitis during the onset of anterior uveitis, and the level of IL-32 is significantly higher than that in the stable stage, which confirms that IL-16, IL-32 and the activity of anterior uveitis are related to the serum IL of the patients with.4 anterior uveitis after treatment. -16, IL-32 level was significantly lower than before treatment, and further confirmed IL-16, IL-32 participated in the occurrence and development of anterior uveitis,.5 serum was positively correlated with IL-16 and IL-32, which was considered to be involved in the occurrence and evolution of anterior uveitis respectively.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R773
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