本文選題：噪聲性聾 + 暫時(shí)性閾移　； 參考：《復(fù)旦學(xué)報(bào)(醫(yī)學(xué)版)》2017年02期

【摘要】：目的探討噪聲暴露后出現(xiàn)暫時(shí)性閾移的小鼠在聽覺反應(yīng)閾值完全恢復(fù)正常后耳蝸內(nèi)毛細(xì)胞突觸的病理改變。方法將小鼠分為正常對照組和實(shí)驗(yàn)組,實(shí)驗(yàn)組小鼠進(jìn)行強(qiáng)度98 dB SPL、時(shí)長2 h的噪聲暴露,建立暫時(shí)性閾移模型。將對照組和暫時(shí)性閾移模型小鼠進(jìn)行全基底膜鋪片并行免疫熒光染色,共聚焦顯微鏡拍照成像并觀察細(xì)胞形態(tài)。利用耳蝸長度計(jì)算耳蝸所處的頻率位置,最后用圖形處理軟件對突觸結(jié)構(gòu)進(jìn)行三維重建,觀察對照組小鼠和實(shí)驗(yàn)組小鼠的耳蝸內(nèi)毛細(xì)胞突觸前、后結(jié)構(gòu)的空間分布規(guī)律及其病理改變特點(diǎn)。結(jié)果對照組小鼠耳蝸內(nèi)毛細(xì)胞的突觸復(fù)合體有明顯的分布特點(diǎn),每個(gè)內(nèi)毛細(xì)胞通過5~30個(gè)突觸與耳蝸神經(jīng)纖維形成突觸連接,靠近蝸軸側(cè)的突觸前結(jié)構(gòu)體積較大,與之相匹配的突觸后結(jié)構(gòu)體積較小�？拷�(xì)胞側(cè)的突觸前結(jié)構(gòu)體積偏小,與之相匹配的突觸后結(jié)構(gòu)體積偏大。實(shí)驗(yàn)組小鼠在高強(qiáng)度噪聲暴露后第2天聽覺測試結(jié)果顯示,聽性腦干反應(yīng)(auditory brain-stem response,ABR)和畸變產(chǎn)物耳聲發(fā)射(distortion product otoacoustic emission,DPOAE)閾值升高30~40 dB,2周后再次測試顯示閾值恢復(fù)正常,但是ABR的Ⅰ波波幅下降46.1%,且耳蝸內(nèi)毛細(xì)胞的突觸復(fù)合體結(jié)構(gòu)數(shù)量也減少了41.3%。而內(nèi)毛細(xì)胞和螺旋神經(jīng)節(jié)細(xì)胞沒有明顯丟失。結(jié)論聽覺閾值功能測試對判斷小鼠耳蝸內(nèi)毛細(xì)胞突觸的損害和丟失不敏感;而聽覺閾上功能測試對評價(jià)小鼠耳蝸內(nèi)毛細(xì)胞突觸的損害和丟失相對敏感。
[Abstract]:Objective to investigate the pathological changes of the synapses of cochlear hair cells in mice with transient threshold shift after noise exposure. Methods the mice were divided into normal control group and experimental group. The experimental group mice were exposed to noise of 98 dB SPLL for 2 hours to establish a temporary threshold shift model. The control group and temporary threshold shift model mice were used to prepare the whole basement membrane and immunofluorescence staining. The confocal microscope was used to photograph and observe the morphology of the cells. The frequency position of cochlea was calculated by cochlea length. Finally, the synaptic structure was reconstructed by graphics processing software, and the presynaptic position of cochlear hair cells was observed in mice of control group and experimental group. The spatial distribution of post-structure and its pathological changes. Results the synaptic complex of the cochlear hair cells in the control group had obvious distribution characteristics. Each of the inner hair cells formed synaptic connections with the cochlear nerve fibers through 5 ~ 30 synapses, and the presynaptic structure near the cochlear axis was larger in volume. The corresponding postsynaptic structure is smaller in size. The volume of the presynaptic structure near the column cell side is smaller than that of the corresponding postsynaptic structure. The results of auditory tests on the second day after high intensity noise exposure showed that auditory brainstem response (auditory brain-stem responseABR) and distortion product otoacoustic emission (DPOAE) thresholds increased 30 ~ 40 dB ~ (2) weeks later, and showed that the thresholds returned to normal again, and the results showed that the auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) thresholds increased 30 ~ 40 dB ~ (2) weeks after exposure. But the amplitude of wave I of ABR decreased by 46.1 and the number of synaptic complex of hair cells in cochlea decreased by 41.3%. There was no significant loss of inner hair cells and spiral ganglion cells. Conclusion the auditory threshold function test is not sensitive to the assessment of synapse damage and loss of mouse cochlear hair cells, while the auditory suprathreshold function test is relatively sensitive to the evaluation of the damage and loss of mouse cochlear hair cell synapses.
【作者單位】： 復(fù)旦大學(xué)附屬眼耳鼻喉醫(yī)院耳鼻喉科;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(81271084)~~
【分類號】：R764.43

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