本文選題：水通道蛋白　切入點：醛固酮　出處：《遼寧醫(yī)學(xué)院》2012年碩士論文

【摘要】：目的 制備豚鼠膜迷路積水動物模型,并對動物進(jìn)行中耳加壓治療，觀察中耳加壓后膜迷路積水的變化及AQP1和AQP2表達(dá)的變化，以探究AQPs在積水過程中的可能作用及中耳加壓對膜迷路積水影響的可能機制。 方法 采用腹腔注射醛固酮方法制備豚鼠膜迷路積水動物模型，將50只豚鼠隨機分為5組，每組各10只，對照組及加壓對照組生理鹽水腹腔注射0.5ml/d，連續(xù)5d；積水4周組，積水8周組，加壓組第1周腹腔注射醛固酮0.1mg/(100g/d),連續(xù)5d。加壓組及加壓對照組豚鼠在造模后第5周起置于壓力艙內(nèi)加壓治療，共3周，觀察每組動物耳蝸形態(tài)和功能的變化，并用SP法免疫組織化學(xué)及Western blot方法檢測各組豚鼠內(nèi)耳AQP1、AQP2的表達(dá)變化。 結(jié)果 1、積水8周組ABR聽閾值為[47.50±4.25]dBSPL比積水4周組[36.50±4.74]dBSPL、加壓對照組[24.95±2.35] dBSPL和對照組[23.52±2.41] dBSPL升高(P0.05)，加壓組[33.50±2.42] dBSPL較積水8周組閾值下降(P0.05)。 2、膜迷路積水程度：對照組及加壓對照組無膜迷路積水；積水8周組R值為[0.63±0.11]較積水4周組[0.47±0.08]及加壓組[0.42±0.05]升高（P0.05）。 3、免疫組化實驗結(jié)果：①AQP1表達(dá)主要在螺旋韌帶基底部、Corti’s器基底膜等處，積水8周組表達(dá)面積比值為[0.0946±0.0125]較積水4周組[0.1163±0.0148]、對照組[0.1492±0.0125]豚鼠AQP1的表達(dá)減少（P0.05），加壓組[0.1216±0.0118]與積水8周組比較AQP1的表達(dá)增加（P0.05）。②AQP2表達(dá)主要在血管紋、螺旋韌帶、Corti’s器等處，積水8周組表達(dá)面積比值為[0.2538±0.0157]較積水4周組[0.1743±0.0064]、對照組[0.1191±0.0085]表達(dá)增加（P0.05），加壓組[0.1502±0.0112]與積水8周組比較AQP2的表達(dá)減少（P0.05）。 4、Western blot實驗結(jié)果：①耳蝸外側(cè)壁組織AQP1表達(dá)量在積水8周組[0.674±0.25]較積水4周組[0.856±0.12]和對照組[0.978±0.15]減少（P0.05），，加壓組[0.753±0.32]與積水8周組比較表達(dá)量增加（P0.05）。②耳蝸外側(cè)壁組織AQP2表達(dá)量在積水8周組[3.507±0.89]較積水4周組[2.194±0.75]及對照組[0.985±0.15]增加（P0.05），加壓組[1.891±0.31]與積水8周組比較表達(dá)量減少（P0.05）。 結(jié)論 1、中耳加壓可減輕醛固酮引起的豚鼠膜迷路積水，并且改善了耳蝸聽功能。 2、由腹腔注射醛固酮引起豚鼠膜迷路積水時，在實驗周期內(nèi)積水隨時間延長而加重，AQP1表達(dá)下調(diào)，而AQP2表達(dá)增加。 3、中耳加壓治療使膜迷路積水豚鼠耳蝸AQP1表達(dá)增加，AQP2表達(dá)減少，說明中耳加壓減輕膜迷路積水可能與AQP的表達(dá)改變有關(guān)。
[Abstract]:PurposeThe animal model of membranous labyrinth hydrops in guinea pigs was established and treated with middle ear pressure. The changes of membranous labyrinthine hydrops and the expression of AQP1 and AQP2 were observed.To explore the possible role of AQPs in hydrops and the possible mechanism of the effects of middle ear pressure on membranous labyrinthine hydrops.Method50 guinea pigs were randomly divided into 5 groups by intraperitoneal injection of aldosterone. The control group and the pressurized control group were injected with 0.5 ml / d normal saline for 5 consecutive days, and the hydrops for 4 weeks were treated with hydrocephalus for 8 weeks, the control group and the pressurized control group were injected intraperitoneally with 0.5 ml / d of normal saline for 5 days.In the first week, aldosterone was injected intraperitoneally with 0.1 mg / 100 g / d aldosterone for 5 days.The guinea pigs in the compression group and the control group were placed in the pressure chamber for 3 weeks from the 5th week after the establishment of the model. The changes of cochlea morphology and function in each group were observed.The expression of AQP2 in the inner ear of guinea pigs was detected by SP immunohistochemical method and Western blot method.Result1,縐按8鍛ㄧ粍ABR鍚槇鍊間負(fù)[47.50鹵4.25]dBSPL姣旂Н姘

本文編號：1712730