本文選題：Delta樣配體　切入點：血管內皮細胞生長因子受體　出處：《中國兒童保健雜志》2016年08期

【摘要】：目的分析Delta樣配體4、血管內皮細胞生長因子受體1、血管內皮細胞生長因子受體2在視網膜新生血管中的表達,探討Notch1-Dll4信號通路在氧誘導視網膜病小鼠新生血管形成中的作用。方法選用鼠齡7d的C57BL/6J新生鼠30只,分成實驗組和對照組。實驗組15只,在氧濃度為(75±2)%的密閉容器中生長5d,回到室內正�？諝庵�;對照組15只,在室內正�？諝庵猩L。兩組各取p7(生后7d)、p12、p17新生鼠5只,摘除眼球,免疫組化法進行Dll4、VEGFR-1、VEGFR-2的測定。結果免疫組化結果顯示,對照組和實驗組Dll4與VEGFR-1、VEGFR-2在視網膜均可見陽性細胞表達,p7和p12時,兩組間VEGFR-1細胞的陽性率差異無統(tǒng)計學意義(P0.05);而在p17時,兩組間VEGFR-1細胞的陽性率有差異,實驗組低于對照組(P0.05);p7、p12和p17時,兩組間VEGFR-2細胞的陽性率差異均無統(tǒng)計學意義(P0.05);p7時,兩組間Dll4細胞的陽性率差異無統(tǒng)計學意義(P0.05);而在p12和p17時,兩組間Dll4細胞的陽性率差異有統(tǒng)計學意義(P120.05,P170.001),實驗組的陽性率低于對照組;實驗組不同時間點VEGFR-1和Dll4的陽性細胞率均有降低趨勢(P均0.001),VEGFR-2陽性細胞率有增高趨勢(P0.05)。對照組不同時間點VEGFR-1的陽性細胞率有降低趨勢(P0.05),不同時間點VEGFR-2的陽性細胞率有增高趨勢(P0.001),不同時間點Dll4的陽性細胞率沒有變化趨勢(P0.05)。結論 Notch1-Dll4信號通路可能參與了VEGF調控視網膜新生血管生成的過程,Dll4在氧誘導視網膜病小鼠新生血管的形成中表達受到抑制,對VEGF未能進行有效負反饋調控;VEGFR-1的表達受到抑制,且與Dll4表達趨勢變化一致。
[Abstract]:Objective to investigate the expression of Delta like ligand 4, vascular endothelial growth factor receptor 1 and vascular endothelial growth factor receptor 2 in retinal neovascularization. To investigate the role of Notch1-Dll4 signaling pathway in angiogenesis of retinopathy mice induced by oxygen, 30 C57BL/6J neonate rats aged 7 days were divided into two groups: experimental group (n = 15) and control group (n = 15). After 5 days of growth in a closed container with oxygen concentration of 75 鹵2%, 15 rats in the control group grew in normal indoor air. 5 newborn mice were taken from each group (7 days after birth) and their eyeballs were removed. Immunohistochemical method was used to detect VEGFR-2 in Dll4VEGFR-1 and VEGFR-1VEGFR-2. Results Immunohistochemical results showed that there was no significant difference in the expression of p7 and p12 in Dll4 and VEGFR-1VEGFR-2 in the retina between the two groups, but there was no significant difference in the positive rate of VEGFR-1 cells between the two groups at p17. The positive rate of VEGFR-1 cells in the experimental group was lower than that in the control group (P 0.05, p7p12 and p17). There was no significant difference in the positive rate of VEGFR-2 cells between the two groups. There was no significant difference in the positive rate of Dll4 cells between the two groups at the time of p12 and p17, but at p12 and p17, there was no significant difference in the positive rate of Dll4 cells between the two groups. The positive rate of Dll4 cells in the two groups was significantly higher than that in the control group (P 120.05), and the positive rate in the experimental group was lower than that in the control group. The positive cell rate of VEGFR-1 and Dll4 decreased at different time points in the experimental group. The positive cell rate of VEGFR-2 increased with P 0.001. The positive cell rate of VEGFR-1 decreased at different time points in the control group, and the positive cell rate of VEGFR-2 was decreased at different time points in the control group. The positive cell rate of VEGFR-2 in the control group decreased at different time points, and the positive cell rate of VEGFR-2 at different time points in the control group. The positive cell rate of Dll4 has no change trend at different time points. Conclusion Notch1-Dll4 signaling pathway may be involved in the regulation of retinal angiogenesis by VEGF and Dll4 in oxygen-induced retinopathy mice neovascularization. Its expression is suppressed, The expression of VEGFR-1 was inhibited by negative feedback on VEGF, which was consistent with the trend of Dll4 expression.
【作者單位】： 中山大學附屬第一醫(yī)院兒科;中山大學附屬第一醫(yī)院眼科;
【基金】：2014年廣東省公益研究與能力建設專項資金(2014A020212430)
【分類號】：R774.1;R-332

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